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Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor
We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclon...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660547/ https://www.ncbi.nlm.nih.gov/pubmed/37987253 http://dx.doi.org/10.3390/antib12040075 |
| _version_ | 1785148413308829696 |
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| author | Vester, Susan K. Davies, Anna M. Beavil, Rebecca L. Sandhar, Balraj S. Beavil, Andrew J. Gould, Hannah J. Sutton, Brian J. McDonnell, James M. |
| author_facet | Vester, Susan K. Davies, Anna M. Beavil, Rebecca L. Sandhar, Balraj S. Beavil, Andrew J. Gould, Hannah J. Sutton, Brian J. McDonnell, James M. |
| author_sort | Vester, Susan K. |
| collection | PubMed |
| description | We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described. |
| format | Online Article Text |
| id | pubmed-10660547 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | MDPI |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-106605472023-11-16 Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor Vester, Susan K. Davies, Anna M. Beavil, Rebecca L. Sandhar, Balraj S. Beavil, Andrew J. Gould, Hannah J. Sutton, Brian J. McDonnell, James M. Antibodies (Basel) Article We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described. MDPI 2023-11-16 /pmc/articles/PMC10660547/ /pubmed/37987253 http://dx.doi.org/10.3390/antib12040075 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Vester, Susan K. Davies, Anna M. Beavil, Rebecca L. Sandhar, Balraj S. Beavil, Andrew J. Gould, Hannah J. Sutton, Brian J. McDonnell, James M. Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor |
| title | Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor |
| title_full | Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor |
| title_fullStr | Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor |
| title_full_unstemmed | Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor |
| title_short | Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor |
| title_sort | expanding the anti-phl p 7 antibody toolkit: an anti-idiotype nanobody inhibitor |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660547/ https://www.ncbi.nlm.nih.gov/pubmed/37987253 http://dx.doi.org/10.3390/antib12040075 |
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