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Single-cell and spatial architecture of primary liver cancer
Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transcriptomic and bulk multi-omics, we elaborated a...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661180/ https://www.ncbi.nlm.nih.gov/pubmed/37985711 http://dx.doi.org/10.1038/s42003-023-05455-0 |
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author | Zhou, Pei-Yun Zhou, Cheng Gan, Wei Tang, Zheng Sun, Bao-Ye Huang, Jin-Long Liu, Gao Liu, Wei-Ren Tian, Meng-Xin Jiang, Xi-Fei Wang, Han Tao, Chen-Yang Fang, Yuan Qu, Wei-Feng Huang, Run Zhu, Gui-Qi Huang, Cheng Fu, Xiu-Tao Ding, Zhen-Bin Gao, Qiang Zhou, Jian Shi, Ying-Hong Yi, Yong Fan, Jia Qiu, Shuang-Jian |
author_facet | Zhou, Pei-Yun Zhou, Cheng Gan, Wei Tang, Zheng Sun, Bao-Ye Huang, Jin-Long Liu, Gao Liu, Wei-Ren Tian, Meng-Xin Jiang, Xi-Fei Wang, Han Tao, Chen-Yang Fang, Yuan Qu, Wei-Feng Huang, Run Zhu, Gui-Qi Huang, Cheng Fu, Xiu-Tao Ding, Zhen-Bin Gao, Qiang Zhou, Jian Shi, Ying-Hong Yi, Yong Fan, Jia Qiu, Shuang-Jian |
author_sort | Zhou, Pei-Yun |
collection | PubMed |
description | Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transcriptomic and bulk multi-omics, we elaborated a molecular architecture of 3 PLC types, namely hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and combined hepatocellular-cholangiocarcinoma (CHC). Taking a high-resolution perspective, our observations revealed that CHC cells exhibit internally discordant phenotypes, whereas ICC and HCC exhibit distinct tumor-specific features. Specifically, ICC was found to be the primary source of cancer-associated fibroblasts, while HCC exhibited disrupted metabolism and greater individual heterogeneity of T cells. We further revealed a diversity of intermediate-state cells residing in the tumor-peritumor junctional zone, including a congregation of CPE(+) intermediate-state endothelial cells (ECs), which harbored the molecular characteristics of tumor-associated ECs and normal ECs. This architecture offers insights into molecular characteristics of PLC microenvironment, and hints that the tumor-peritumor junctional zone could serve as a targeted region for precise therapeutical strategies. |
format | Online Article Text |
id | pubmed-10661180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106611802023-11-20 Single-cell and spatial architecture of primary liver cancer Zhou, Pei-Yun Zhou, Cheng Gan, Wei Tang, Zheng Sun, Bao-Ye Huang, Jin-Long Liu, Gao Liu, Wei-Ren Tian, Meng-Xin Jiang, Xi-Fei Wang, Han Tao, Chen-Yang Fang, Yuan Qu, Wei-Feng Huang, Run Zhu, Gui-Qi Huang, Cheng Fu, Xiu-Tao Ding, Zhen-Bin Gao, Qiang Zhou, Jian Shi, Ying-Hong Yi, Yong Fan, Jia Qiu, Shuang-Jian Commun Biol Article Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transcriptomic and bulk multi-omics, we elaborated a molecular architecture of 3 PLC types, namely hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and combined hepatocellular-cholangiocarcinoma (CHC). Taking a high-resolution perspective, our observations revealed that CHC cells exhibit internally discordant phenotypes, whereas ICC and HCC exhibit distinct tumor-specific features. Specifically, ICC was found to be the primary source of cancer-associated fibroblasts, while HCC exhibited disrupted metabolism and greater individual heterogeneity of T cells. We further revealed a diversity of intermediate-state cells residing in the tumor-peritumor junctional zone, including a congregation of CPE(+) intermediate-state endothelial cells (ECs), which harbored the molecular characteristics of tumor-associated ECs and normal ECs. This architecture offers insights into molecular characteristics of PLC microenvironment, and hints that the tumor-peritumor junctional zone could serve as a targeted region for precise therapeutical strategies. Nature Publishing Group UK 2023-11-20 /pmc/articles/PMC10661180/ /pubmed/37985711 http://dx.doi.org/10.1038/s42003-023-05455-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhou, Pei-Yun Zhou, Cheng Gan, Wei Tang, Zheng Sun, Bao-Ye Huang, Jin-Long Liu, Gao Liu, Wei-Ren Tian, Meng-Xin Jiang, Xi-Fei Wang, Han Tao, Chen-Yang Fang, Yuan Qu, Wei-Feng Huang, Run Zhu, Gui-Qi Huang, Cheng Fu, Xiu-Tao Ding, Zhen-Bin Gao, Qiang Zhou, Jian Shi, Ying-Hong Yi, Yong Fan, Jia Qiu, Shuang-Jian Single-cell and spatial architecture of primary liver cancer |
title | Single-cell and spatial architecture of primary liver cancer |
title_full | Single-cell and spatial architecture of primary liver cancer |
title_fullStr | Single-cell and spatial architecture of primary liver cancer |
title_full_unstemmed | Single-cell and spatial architecture of primary liver cancer |
title_short | Single-cell and spatial architecture of primary liver cancer |
title_sort | single-cell and spatial architecture of primary liver cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661180/ https://www.ncbi.nlm.nih.gov/pubmed/37985711 http://dx.doi.org/10.1038/s42003-023-05455-0 |
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