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Single-cell and spatial architecture of primary liver cancer

Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transcriptomic and bulk multi-omics, we elaborated a...

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Autores principales: Zhou, Pei-Yun, Zhou, Cheng, Gan, Wei, Tang, Zheng, Sun, Bao-Ye, Huang, Jin-Long, Liu, Gao, Liu, Wei-Ren, Tian, Meng-Xin, Jiang, Xi-Fei, Wang, Han, Tao, Chen-Yang, Fang, Yuan, Qu, Wei-Feng, Huang, Run, Zhu, Gui-Qi, Huang, Cheng, Fu, Xiu-Tao, Ding, Zhen-Bin, Gao, Qiang, Zhou, Jian, Shi, Ying-Hong, Yi, Yong, Fan, Jia, Qiu, Shuang-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661180/
https://www.ncbi.nlm.nih.gov/pubmed/37985711
http://dx.doi.org/10.1038/s42003-023-05455-0
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author Zhou, Pei-Yun
Zhou, Cheng
Gan, Wei
Tang, Zheng
Sun, Bao-Ye
Huang, Jin-Long
Liu, Gao
Liu, Wei-Ren
Tian, Meng-Xin
Jiang, Xi-Fei
Wang, Han
Tao, Chen-Yang
Fang, Yuan
Qu, Wei-Feng
Huang, Run
Zhu, Gui-Qi
Huang, Cheng
Fu, Xiu-Tao
Ding, Zhen-Bin
Gao, Qiang
Zhou, Jian
Shi, Ying-Hong
Yi, Yong
Fan, Jia
Qiu, Shuang-Jian
author_facet Zhou, Pei-Yun
Zhou, Cheng
Gan, Wei
Tang, Zheng
Sun, Bao-Ye
Huang, Jin-Long
Liu, Gao
Liu, Wei-Ren
Tian, Meng-Xin
Jiang, Xi-Fei
Wang, Han
Tao, Chen-Yang
Fang, Yuan
Qu, Wei-Feng
Huang, Run
Zhu, Gui-Qi
Huang, Cheng
Fu, Xiu-Tao
Ding, Zhen-Bin
Gao, Qiang
Zhou, Jian
Shi, Ying-Hong
Yi, Yong
Fan, Jia
Qiu, Shuang-Jian
author_sort Zhou, Pei-Yun
collection PubMed
description Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transcriptomic and bulk multi-omics, we elaborated a molecular architecture of 3 PLC types, namely hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and combined hepatocellular-cholangiocarcinoma (CHC). Taking a high-resolution perspective, our observations revealed that CHC cells exhibit internally discordant phenotypes, whereas ICC and HCC exhibit distinct tumor-specific features. Specifically, ICC was found to be the primary source of cancer-associated fibroblasts, while HCC exhibited disrupted metabolism and greater individual heterogeneity of T cells. We further revealed a diversity of intermediate-state cells residing in the tumor-peritumor junctional zone, including a congregation of CPE(+) intermediate-state endothelial cells (ECs), which harbored the molecular characteristics of tumor-associated ECs and normal ECs. This architecture offers insights into molecular characteristics of PLC microenvironment, and hints that the tumor-peritumor junctional zone could serve as a targeted region for precise therapeutical strategies.
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spelling pubmed-106611802023-11-20 Single-cell and spatial architecture of primary liver cancer Zhou, Pei-Yun Zhou, Cheng Gan, Wei Tang, Zheng Sun, Bao-Ye Huang, Jin-Long Liu, Gao Liu, Wei-Ren Tian, Meng-Xin Jiang, Xi-Fei Wang, Han Tao, Chen-Yang Fang, Yuan Qu, Wei-Feng Huang, Run Zhu, Gui-Qi Huang, Cheng Fu, Xiu-Tao Ding, Zhen-Bin Gao, Qiang Zhou, Jian Shi, Ying-Hong Yi, Yong Fan, Jia Qiu, Shuang-Jian Commun Biol Article Primary liver cancer (PLC) poses a leading threat to human health, and its treatment options are limited. Meanwhile, the investigation of homogeneity and heterogeneity among PLCs remains challenging. Here, using single-cell RNA sequencing, spatial transcriptomic and bulk multi-omics, we elaborated a molecular architecture of 3 PLC types, namely hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and combined hepatocellular-cholangiocarcinoma (CHC). Taking a high-resolution perspective, our observations revealed that CHC cells exhibit internally discordant phenotypes, whereas ICC and HCC exhibit distinct tumor-specific features. Specifically, ICC was found to be the primary source of cancer-associated fibroblasts, while HCC exhibited disrupted metabolism and greater individual heterogeneity of T cells. We further revealed a diversity of intermediate-state cells residing in the tumor-peritumor junctional zone, including a congregation of CPE(+) intermediate-state endothelial cells (ECs), which harbored the molecular characteristics of tumor-associated ECs and normal ECs. This architecture offers insights into molecular characteristics of PLC microenvironment, and hints that the tumor-peritumor junctional zone could serve as a targeted region for precise therapeutical strategies. Nature Publishing Group UK 2023-11-20 /pmc/articles/PMC10661180/ /pubmed/37985711 http://dx.doi.org/10.1038/s42003-023-05455-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhou, Pei-Yun
Zhou, Cheng
Gan, Wei
Tang, Zheng
Sun, Bao-Ye
Huang, Jin-Long
Liu, Gao
Liu, Wei-Ren
Tian, Meng-Xin
Jiang, Xi-Fei
Wang, Han
Tao, Chen-Yang
Fang, Yuan
Qu, Wei-Feng
Huang, Run
Zhu, Gui-Qi
Huang, Cheng
Fu, Xiu-Tao
Ding, Zhen-Bin
Gao, Qiang
Zhou, Jian
Shi, Ying-Hong
Yi, Yong
Fan, Jia
Qiu, Shuang-Jian
Single-cell and spatial architecture of primary liver cancer
title Single-cell and spatial architecture of primary liver cancer
title_full Single-cell and spatial architecture of primary liver cancer
title_fullStr Single-cell and spatial architecture of primary liver cancer
title_full_unstemmed Single-cell and spatial architecture of primary liver cancer
title_short Single-cell and spatial architecture of primary liver cancer
title_sort single-cell and spatial architecture of primary liver cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661180/
https://www.ncbi.nlm.nih.gov/pubmed/37985711
http://dx.doi.org/10.1038/s42003-023-05455-0
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