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Unraveling the salt tolerance of Phi29 DNA polymerase using compartmentalized self-replication and microfluidics platform

In Phi29-α–hemolysin (α-HL) nanopore sequencing systems, a strong electrochemical signal is dependent on a high concentration of salt. However, high salt concentrations adversely affect polymerase activity. Sequencing by synthesis (SBS) requires the use of phi29 polymerase without exonuclease activi...

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Detalles Bibliográficos
Autores principales:	Sun, Yaping, Ko, Danny Hsu, Gao, Jie, Fu, Kang, Gao, Yaping, Zhang, Qiwen, Baldi, Salem, Hong, Tao, Ivanov, Igor, He, Yun, Tian, Hui
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2023
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661337/ https://www.ncbi.nlm.nih.gov/pubmed/38029082 http://dx.doi.org/10.3389/fmicb.2023.1267196

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