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Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance

The sensitivity of NMR may be enhanced by more than four orders of magnitude via dissolution dynamic nuclear polarization (dDNP), potentially allowing real-time, in situ analysis of chemical reactions. However, there has been no widespread use of the technique for this application and the major limi...

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Autores principales: Yeste, Jose, Azagra, Marc, Ortega, Maria A., Portela, Alejandro, Matajsz, Gergő, Herrero-Gómez, Alba, Kim, Yaewon, Sriram, Renuka, Kurhanewicz, John, Vigneron, Daniel B., Marco-Rius, Irene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661666/
https://www.ncbi.nlm.nih.gov/pubmed/37906028
http://dx.doi.org/10.1039/d3lc00474k
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author Yeste, Jose
Azagra, Marc
Ortega, Maria A.
Portela, Alejandro
Matajsz, Gergő
Herrero-Gómez, Alba
Kim, Yaewon
Sriram, Renuka
Kurhanewicz, John
Vigneron, Daniel B.
Marco-Rius, Irene
author_facet Yeste, Jose
Azagra, Marc
Ortega, Maria A.
Portela, Alejandro
Matajsz, Gergő
Herrero-Gómez, Alba
Kim, Yaewon
Sriram, Renuka
Kurhanewicz, John
Vigneron, Daniel B.
Marco-Rius, Irene
author_sort Yeste, Jose
collection PubMed
description The sensitivity of NMR may be enhanced by more than four orders of magnitude via dissolution dynamic nuclear polarization (dDNP), potentially allowing real-time, in situ analysis of chemical reactions. However, there has been no widespread use of the technique for this application and the major limitation has been the low experimental throughput caused by the time-consuming polarization build-up process at cryogenic temperatures and fast decay of the hyper-intense signal post dissolution. To overcome this limitation, we have developed a microfluidic device compatible with dDNP-MR spectroscopic imaging methods for detection of reactants and products in chemical reactions in which up to 8 reactions can be measured simultaneously using a single dDNP sample. Multiple MR spectroscopic data sets can be generated under the same exact conditions of hyperpolarized solute polarization, concentration, pH, and temperature. A proof-of-concept for the technology is demonstrated by identifying the reactants in the decarboxylation of pyruvate via hydrogen peroxide (e.g. 2-hydroperoxy-2-hydroxypropanoate, peroxymonocarbonate and CO(2)). dDNP-MR allows tracing of fast chemical reactions that would be barely detectable at thermal equilibrium by MR. We envisage that dDNP-MR spectroscopic imaging combined with microfluidics will provide a new high-throughput method for dDNP enhanced MR analysis of multiple components in chemical reactions and for non-destructive in situ metabolic analysis of hyperpolarized substrates in biological samples for laboratory and preclinical research.
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spelling pubmed-106616662023-10-19 Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance Yeste, Jose Azagra, Marc Ortega, Maria A. Portela, Alejandro Matajsz, Gergő Herrero-Gómez, Alba Kim, Yaewon Sriram, Renuka Kurhanewicz, John Vigneron, Daniel B. Marco-Rius, Irene Lab Chip Chemistry The sensitivity of NMR may be enhanced by more than four orders of magnitude via dissolution dynamic nuclear polarization (dDNP), potentially allowing real-time, in situ analysis of chemical reactions. However, there has been no widespread use of the technique for this application and the major limitation has been the low experimental throughput caused by the time-consuming polarization build-up process at cryogenic temperatures and fast decay of the hyper-intense signal post dissolution. To overcome this limitation, we have developed a microfluidic device compatible with dDNP-MR spectroscopic imaging methods for detection of reactants and products in chemical reactions in which up to 8 reactions can be measured simultaneously using a single dDNP sample. Multiple MR spectroscopic data sets can be generated under the same exact conditions of hyperpolarized solute polarization, concentration, pH, and temperature. A proof-of-concept for the technology is demonstrated by identifying the reactants in the decarboxylation of pyruvate via hydrogen peroxide (e.g. 2-hydroperoxy-2-hydroxypropanoate, peroxymonocarbonate and CO(2)). dDNP-MR allows tracing of fast chemical reactions that would be barely detectable at thermal equilibrium by MR. We envisage that dDNP-MR spectroscopic imaging combined with microfluidics will provide a new high-throughput method for dDNP enhanced MR analysis of multiple components in chemical reactions and for non-destructive in situ metabolic analysis of hyperpolarized substrates in biological samples for laboratory and preclinical research. The Royal Society of Chemistry 2023-10-19 /pmc/articles/PMC10661666/ /pubmed/37906028 http://dx.doi.org/10.1039/d3lc00474k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Yeste, Jose
Azagra, Marc
Ortega, Maria A.
Portela, Alejandro
Matajsz, Gergő
Herrero-Gómez, Alba
Kim, Yaewon
Sriram, Renuka
Kurhanewicz, John
Vigneron, Daniel B.
Marco-Rius, Irene
Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance
title Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance
title_full Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance
title_fullStr Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance
title_full_unstemmed Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance
title_short Parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance
title_sort parallel detection of chemical reactions in a microfluidic platform using hyperpolarized nuclear magnetic resonance
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661666/
https://www.ncbi.nlm.nih.gov/pubmed/37906028
http://dx.doi.org/10.1039/d3lc00474k
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