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Brain stimulation-on-a-chip: a neuromodulation platform for brain slices

Electrical stimulation of ex vivo brain tissue slices has been a method used to understand mechanisms imparted by transcranial direct current stimulation (tDCS), but there are significant direct current electric field (dcEF) dosage and electrochemical by-product concerns in conventional experimental...

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Autores principales: Shaner, Sebastian, Lu, Han, Lenz, Maximilian, Garg, Shreyash, Vlachos, Andreas, Asplund, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661668/
https://www.ncbi.nlm.nih.gov/pubmed/37909911
http://dx.doi.org/10.1039/d3lc00492a
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author Shaner, Sebastian
Lu, Han
Lenz, Maximilian
Garg, Shreyash
Vlachos, Andreas
Asplund, Maria
author_facet Shaner, Sebastian
Lu, Han
Lenz, Maximilian
Garg, Shreyash
Vlachos, Andreas
Asplund, Maria
author_sort Shaner, Sebastian
collection PubMed
description Electrical stimulation of ex vivo brain tissue slices has been a method used to understand mechanisms imparted by transcranial direct current stimulation (tDCS), but there are significant direct current electric field (dcEF) dosage and electrochemical by-product concerns in conventional experimental setups that may impact translational findings. Therefore, we developed an on-chip platform with fluidic, electrochemical, and magnetically-induced spatial control. Fluidically, the chamber geometrically confines precise dcEF delivery to the enclosed brain slice and allows for tissue recovery in order to monitor post-stimulation effects. Electrochemically, conducting hydrogel electrodes mitigate stimulation-induced faradaic reactions typical of commonly-used metal electrodes. Magnetically, we applied ferromagnetic substrates beneath the tissue and used an external permanent magnet to enable in situ rotational control in relation to the dcEF. By combining the microfluidic chamber with live-cell calcium imaging and electrophysiological recordings, we showcased the potential to study the acute and lasting effects of dcEFs with the potential of providing multi-session stimulation. This on-chip bioelectronic platform presents a modernized yet simple solution to electrically stimulate explanted tissue by offering more environmental control to users, which unlocks new opportunities to conduct thorough brain stimulation mechanistic investigations.
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spelling pubmed-106616682023-10-30 Brain stimulation-on-a-chip: a neuromodulation platform for brain slices Shaner, Sebastian Lu, Han Lenz, Maximilian Garg, Shreyash Vlachos, Andreas Asplund, Maria Lab Chip Chemistry Electrical stimulation of ex vivo brain tissue slices has been a method used to understand mechanisms imparted by transcranial direct current stimulation (tDCS), but there are significant direct current electric field (dcEF) dosage and electrochemical by-product concerns in conventional experimental setups that may impact translational findings. Therefore, we developed an on-chip platform with fluidic, electrochemical, and magnetically-induced spatial control. Fluidically, the chamber geometrically confines precise dcEF delivery to the enclosed brain slice and allows for tissue recovery in order to monitor post-stimulation effects. Electrochemically, conducting hydrogel electrodes mitigate stimulation-induced faradaic reactions typical of commonly-used metal electrodes. Magnetically, we applied ferromagnetic substrates beneath the tissue and used an external permanent magnet to enable in situ rotational control in relation to the dcEF. By combining the microfluidic chamber with live-cell calcium imaging and electrophysiological recordings, we showcased the potential to study the acute and lasting effects of dcEFs with the potential of providing multi-session stimulation. This on-chip bioelectronic platform presents a modernized yet simple solution to electrically stimulate explanted tissue by offering more environmental control to users, which unlocks new opportunities to conduct thorough brain stimulation mechanistic investigations. The Royal Society of Chemistry 2023-10-30 /pmc/articles/PMC10661668/ /pubmed/37909911 http://dx.doi.org/10.1039/d3lc00492a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Shaner, Sebastian
Lu, Han
Lenz, Maximilian
Garg, Shreyash
Vlachos, Andreas
Asplund, Maria
Brain stimulation-on-a-chip: a neuromodulation platform for brain slices
title Brain stimulation-on-a-chip: a neuromodulation platform for brain slices
title_full Brain stimulation-on-a-chip: a neuromodulation platform for brain slices
title_fullStr Brain stimulation-on-a-chip: a neuromodulation platform for brain slices
title_full_unstemmed Brain stimulation-on-a-chip: a neuromodulation platform for brain slices
title_short Brain stimulation-on-a-chip: a neuromodulation platform for brain slices
title_sort brain stimulation-on-a-chip: a neuromodulation platform for brain slices
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661668/
https://www.ncbi.nlm.nih.gov/pubmed/37909911
http://dx.doi.org/10.1039/d3lc00492a
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