Sterility testing of germ-free mouse colonies

In biomedical research, germ-free and gnotobiotic mouse models enable the mechanistic investigation of microbiota-host interactions and their role on (patho)physiology. Throughout any gnotobiotic experiment, standardized and periodic microbiological testing of defined gnotobiotic housing conditions...

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Autores principales: Dremova, Olga, Mimmler, Maximilian, Paeslack, Nadja, Khuu, My Phung, Gao, Zhenling, Bosmann, Markus, Garo, Lucien P., Schön, Nathalie, Mechler, Alexa, Beneich, Yunes, Rebling, Vivian, Mann, Amrit, Pontarollo, Giulia, Kiouptsi, Klytaimnistra, Reinhardt, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662041/
https://www.ncbi.nlm.nih.gov/pubmed/38022683
http://dx.doi.org/10.3389/fimmu.2023.1275109
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author Dremova, Olga
Mimmler, Maximilian
Paeslack, Nadja
Khuu, My Phung
Gao, Zhenling
Bosmann, Markus
Garo, Lucien P.
Schön, Nathalie
Mechler, Alexa
Beneich, Yunes
Rebling, Vivian
Mann, Amrit
Pontarollo, Giulia
Kiouptsi, Klytaimnistra
Reinhardt, Christoph
author_facet Dremova, Olga
Mimmler, Maximilian
Paeslack, Nadja
Khuu, My Phung
Gao, Zhenling
Bosmann, Markus
Garo, Lucien P.
Schön, Nathalie
Mechler, Alexa
Beneich, Yunes
Rebling, Vivian
Mann, Amrit
Pontarollo, Giulia
Kiouptsi, Klytaimnistra
Reinhardt, Christoph
author_sort Dremova, Olga
collection PubMed
description In biomedical research, germ-free and gnotobiotic mouse models enable the mechanistic investigation of microbiota-host interactions and their role on (patho)physiology. Throughout any gnotobiotic experiment, standardized and periodic microbiological testing of defined gnotobiotic housing conditions is a key requirement. Here, we review basic principles of germ-free isolator technology, the suitability of various sterilization methods, and the use of sterility testing methods to monitor germ-free mouse colonies. We also discuss their effectiveness and limitations, and share the experience with protocols used in our facility. In addition, possible sources of isolator contamination are discussed and an overview of reported contaminants is provided.
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spelling pubmed-106620412023-01-01 Sterility testing of germ-free mouse colonies Dremova, Olga Mimmler, Maximilian Paeslack, Nadja Khuu, My Phung Gao, Zhenling Bosmann, Markus Garo, Lucien P. Schön, Nathalie Mechler, Alexa Beneich, Yunes Rebling, Vivian Mann, Amrit Pontarollo, Giulia Kiouptsi, Klytaimnistra Reinhardt, Christoph Front Immunol Immunology In biomedical research, germ-free and gnotobiotic mouse models enable the mechanistic investigation of microbiota-host interactions and their role on (patho)physiology. Throughout any gnotobiotic experiment, standardized and periodic microbiological testing of defined gnotobiotic housing conditions is a key requirement. Here, we review basic principles of germ-free isolator technology, the suitability of various sterilization methods, and the use of sterility testing methods to monitor germ-free mouse colonies. We also discuss their effectiveness and limitations, and share the experience with protocols used in our facility. In addition, possible sources of isolator contamination are discussed and an overview of reported contaminants is provided. Frontiers Media S.A. 2023-11-07 /pmc/articles/PMC10662041/ /pubmed/38022683 http://dx.doi.org/10.3389/fimmu.2023.1275109 Text en Copyright © 2023 Dremova, Mimmler, Paeslack, Khuu, Gao, Bosmann, Garo, Schön, Mechler, Beneich, Rebling, Mann, Pontarollo, Kiouptsi and Reinhardt https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Dremova, Olga
Mimmler, Maximilian
Paeslack, Nadja
Khuu, My Phung
Gao, Zhenling
Bosmann, Markus
Garo, Lucien P.
Schön, Nathalie
Mechler, Alexa
Beneich, Yunes
Rebling, Vivian
Mann, Amrit
Pontarollo, Giulia
Kiouptsi, Klytaimnistra
Reinhardt, Christoph
Sterility testing of germ-free mouse colonies
title Sterility testing of germ-free mouse colonies
title_full Sterility testing of germ-free mouse colonies
title_fullStr Sterility testing of germ-free mouse colonies
title_full_unstemmed Sterility testing of germ-free mouse colonies
title_short Sterility testing of germ-free mouse colonies
title_sort sterility testing of germ-free mouse colonies
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662041/
https://www.ncbi.nlm.nih.gov/pubmed/38022683
http://dx.doi.org/10.3389/fimmu.2023.1275109
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