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Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types
Photosynthesis is increasingly becoming a recognized target for crop improvement. Phenotyping photosynthesis-related traits on field-grown material is a key bottleneck to progress here due to logistical barriers and short measurement days. Many studies attempt to overcome these challenges by phenoty...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662226/ https://www.ncbi.nlm.nih.gov/pubmed/37565685 http://dx.doi.org/10.1093/jxb/erad319 |
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author | Ferguson, John N Jithesh, Tamanna Lawson, Tracy Kromdijk, Johannes |
author_facet | Ferguson, John N Jithesh, Tamanna Lawson, Tracy Kromdijk, Johannes |
author_sort | Ferguson, John N |
collection | PubMed |
description | Photosynthesis is increasingly becoming a recognized target for crop improvement. Phenotyping photosynthesis-related traits on field-grown material is a key bottleneck to progress here due to logistical barriers and short measurement days. Many studies attempt to overcome these challenges by phenotyping excised leaf material in the laboratory. To date there are no demonstrated examples of the representative nature of photosynthesis measurements performed on excised leaves relative to attached leaves in crops. Here, we tested whether standardized leaf excision on the day prior to phenotyping affected a range of common photosynthesis-related traits across crop functional types using tomato (C(3) dicot), barley (C(3) monocot), and maize (C(4) monocot). Potentially constraining aspects of leaf physiology that could be predicted to impair photosynthesis in excised leaves, namely leaf water potential and abscisic acid accumulation, were not different between attached and excised leaves. We also observed non-significant differences in spectral reflectance and chlorophyll fluorescence traits between the treatments across the three species. However, we did observe some significant differences between traits associated with gas exchange and photosynthetic capacity across all three species. This study represents a useful reference for those who perform measurements of this nature and the differences reported should be considered in associated experimental design and statistical analyses. |
format | Online Article Text |
id | pubmed-10662226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-106622262023-08-11 Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types Ferguson, John N Jithesh, Tamanna Lawson, Tracy Kromdijk, Johannes J Exp Bot Research Papers Photosynthesis is increasingly becoming a recognized target for crop improvement. Phenotyping photosynthesis-related traits on field-grown material is a key bottleneck to progress here due to logistical barriers and short measurement days. Many studies attempt to overcome these challenges by phenotyping excised leaf material in the laboratory. To date there are no demonstrated examples of the representative nature of photosynthesis measurements performed on excised leaves relative to attached leaves in crops. Here, we tested whether standardized leaf excision on the day prior to phenotyping affected a range of common photosynthesis-related traits across crop functional types using tomato (C(3) dicot), barley (C(3) monocot), and maize (C(4) monocot). Potentially constraining aspects of leaf physiology that could be predicted to impair photosynthesis in excised leaves, namely leaf water potential and abscisic acid accumulation, were not different between attached and excised leaves. We also observed non-significant differences in spectral reflectance and chlorophyll fluorescence traits between the treatments across the three species. However, we did observe some significant differences between traits associated with gas exchange and photosynthetic capacity across all three species. This study represents a useful reference for those who perform measurements of this nature and the differences reported should be considered in associated experimental design and statistical analyses. Oxford University Press 2023-08-11 /pmc/articles/PMC10662226/ /pubmed/37565685 http://dx.doi.org/10.1093/jxb/erad319 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Ferguson, John N Jithesh, Tamanna Lawson, Tracy Kromdijk, Johannes Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types |
title | Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types |
title_full | Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types |
title_fullStr | Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types |
title_full_unstemmed | Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types |
title_short | Excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types |
title_sort | excised leaves show limited and species-specific effects on photosynthetic parameters across crop functional types |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662226/ https://www.ncbi.nlm.nih.gov/pubmed/37565685 http://dx.doi.org/10.1093/jxb/erad319 |
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