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Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs

Animal African trypanosomosis is an important vector-borne disease of livestock in sub-Saharan Africa. Pigs seem relatively tolerant to trypanosome infection and could act as a reservoir of trypanosomes affecting animals and humans. Our ability to reliably detect trypanosome infection in pigs depend...

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Autores principales: Ilboudo, Kadidiata, Boulangé, Alain, Hounyèmè, Robert Eustache, Gimonneau, Geoffrey, Kaboré, Jacques, Belem, Adrien Gaston Marie, Desquesnes, Marc, Lejon, Veerle, Koffi, Mathurin, Jamonneau, Vincent, Thévenon, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662723/
https://www.ncbi.nlm.nih.gov/pubmed/37943881
http://dx.doi.org/10.1371/journal.pntd.0011730
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author Ilboudo, Kadidiata
Boulangé, Alain
Hounyèmè, Robert Eustache
Gimonneau, Geoffrey
Kaboré, Jacques
Belem, Adrien Gaston Marie
Desquesnes, Marc
Lejon, Veerle
Koffi, Mathurin
Jamonneau, Vincent
Thévenon, Sophie
author_facet Ilboudo, Kadidiata
Boulangé, Alain
Hounyèmè, Robert Eustache
Gimonneau, Geoffrey
Kaboré, Jacques
Belem, Adrien Gaston Marie
Desquesnes, Marc
Lejon, Veerle
Koffi, Mathurin
Jamonneau, Vincent
Thévenon, Sophie
author_sort Ilboudo, Kadidiata
collection PubMed
description Animal African trypanosomosis is an important vector-borne disease of livestock in sub-Saharan Africa. Pigs seem relatively tolerant to trypanosome infection and could act as a reservoir of trypanosomes affecting animals and humans. Our ability to reliably detect trypanosome infection in pigs depends on the performance of diagnostic tools, which is not well known. In pigs experimentally infected with Trypanosoma brucei brucei, we evaluated the performance of parasitological Buffy Coat Technique (BCT), two molecular (TBR and 5.8S PCR) and four serological tests (CATT, HAT Sero-K-Set rapid diagnostic test–RDT, indirect ELISA, immune trypanolysis). Most diagnostic tests showed high specificity, estimated at 100% (95% CI = 74–100%) with the exception of CATT and RDT whose specificity varied between 100% (95% CI = 74–100%) to 50% (95% CI = 7–93%) during the experiment. The sensitivity of each test fluctuated over the course of the infection. The percentage of positive BCT over the infection (30%) was lower than of positive PCR (56% and 62%, depending on primers). Among the serological tests, the percentage of positive tests was 97%, 96%, 86% and 84% for RDT, ELISA, immune trypanolysis and CATT, respectively. Fair agreement was observed between both molecular tests (κ = 0.36). Among the serological tests, the agreement between the ELISA and the RDT was substantial (κ = 0.65). Our results on the T.b. brucei infection model suggest that serological techniques are efficient in detecting the chronic phase of infection, PCR is able to detect positive samples several months after parasites inoculation while BCT becomes negative. BCT examination and RDT are useful to get a quick information in the field, and BCT can be used for treatment decision. ELISA appears most suited for epidemiological studies. The selection of diagnostic tests for trypanosomosis in pigs depends on the context, the objectives and the available resources.
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spelling pubmed-106627232023-11-09 Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs Ilboudo, Kadidiata Boulangé, Alain Hounyèmè, Robert Eustache Gimonneau, Geoffrey Kaboré, Jacques Belem, Adrien Gaston Marie Desquesnes, Marc Lejon, Veerle Koffi, Mathurin Jamonneau, Vincent Thévenon, Sophie PLoS Negl Trop Dis Research Article Animal African trypanosomosis is an important vector-borne disease of livestock in sub-Saharan Africa. Pigs seem relatively tolerant to trypanosome infection and could act as a reservoir of trypanosomes affecting animals and humans. Our ability to reliably detect trypanosome infection in pigs depends on the performance of diagnostic tools, which is not well known. In pigs experimentally infected with Trypanosoma brucei brucei, we evaluated the performance of parasitological Buffy Coat Technique (BCT), two molecular (TBR and 5.8S PCR) and four serological tests (CATT, HAT Sero-K-Set rapid diagnostic test–RDT, indirect ELISA, immune trypanolysis). Most diagnostic tests showed high specificity, estimated at 100% (95% CI = 74–100%) with the exception of CATT and RDT whose specificity varied between 100% (95% CI = 74–100%) to 50% (95% CI = 7–93%) during the experiment. The sensitivity of each test fluctuated over the course of the infection. The percentage of positive BCT over the infection (30%) was lower than of positive PCR (56% and 62%, depending on primers). Among the serological tests, the percentage of positive tests was 97%, 96%, 86% and 84% for RDT, ELISA, immune trypanolysis and CATT, respectively. Fair agreement was observed between both molecular tests (κ = 0.36). Among the serological tests, the agreement between the ELISA and the RDT was substantial (κ = 0.65). Our results on the T.b. brucei infection model suggest that serological techniques are efficient in detecting the chronic phase of infection, PCR is able to detect positive samples several months after parasites inoculation while BCT becomes negative. BCT examination and RDT are useful to get a quick information in the field, and BCT can be used for treatment decision. ELISA appears most suited for epidemiological studies. The selection of diagnostic tests for trypanosomosis in pigs depends on the context, the objectives and the available resources. Public Library of Science 2023-11-09 /pmc/articles/PMC10662723/ /pubmed/37943881 http://dx.doi.org/10.1371/journal.pntd.0011730 Text en © 2023 Ilboudo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ilboudo, Kadidiata
Boulangé, Alain
Hounyèmè, Robert Eustache
Gimonneau, Geoffrey
Kaboré, Jacques
Belem, Adrien Gaston Marie
Desquesnes, Marc
Lejon, Veerle
Koffi, Mathurin
Jamonneau, Vincent
Thévenon, Sophie
Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs
title Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs
title_full Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs
title_fullStr Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs
title_full_unstemmed Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs
title_short Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs
title_sort performance of diagnostic tests for trypanosoma brucei brucei in experimentally infected pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662723/
https://www.ncbi.nlm.nih.gov/pubmed/37943881
http://dx.doi.org/10.1371/journal.pntd.0011730
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