Cargando…

Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia

Ferroptosis is a recently identified form of cell death that is distinct from the conventional modes such as necrosis, apoptosis, and autophagy. Its role in bronchopulmonary dysplasia (BPD) remains inadequately understood. To address this gap, we obtained BPD-related RNA-seq data and ferroptosis-rel...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Xiaoxue, Tao, Ziyu, Chen, Leiming, Duan, Shaozhi, Zhou, Guoping, Ma, Yunxia, Xiong, Zhenqin, Zhu, Lan, Ma, Xuejiao, Mao, Yan, Hu, Yifang, Zeng, Ni, Wang, Jimei, Bao, Yunlei, Luo, Fei, Wu, Chuyan, Jiang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662800/
https://www.ncbi.nlm.nih.gov/pubmed/37478211
http://dx.doi.org/10.1097/MD.0000000000034371
_version_ 1785138276002168832
author Ma, Xiaoxue
Tao, Ziyu
Chen, Leiming
Duan, Shaozhi
Zhou, Guoping
Ma, Yunxia
Xiong, Zhenqin
Zhu, Lan
Ma, Xuejiao
Mao, Yan
Hu, Yifang
Zeng, Ni
Wang, Jimei
Bao, Yunlei
Luo, Fei
Wu, Chuyan
Jiang, Feng
author_facet Ma, Xiaoxue
Tao, Ziyu
Chen, Leiming
Duan, Shaozhi
Zhou, Guoping
Ma, Yunxia
Xiong, Zhenqin
Zhu, Lan
Ma, Xuejiao
Mao, Yan
Hu, Yifang
Zeng, Ni
Wang, Jimei
Bao, Yunlei
Luo, Fei
Wu, Chuyan
Jiang, Feng
author_sort Ma, Xiaoxue
collection PubMed
description Ferroptosis is a recently identified form of cell death that is distinct from the conventional modes such as necrosis, apoptosis, and autophagy. Its role in bronchopulmonary dysplasia (BPD) remains inadequately understood. To address this gap, we obtained BPD-related RNA-seq data and ferroptosis-related genes (FRGs) from the GEO database and FerrDb, respectively. A total of 171 BPD-related differentially expressed ferroptosis-related genes (DE-FRGs) linked to the regulation of autophagy and immune response were identified. Least absolute shrinkage and selection operator and SVM-RFE algorithms identified 23 and 14 genes, respectively, as marker genes. The intersection of these 2 sets yielded 9 genes (ALOX12B, NR1D1, LGMN, IFNA21, MEG3, AKR1C1, CA9, ABCC5, and GALNT14) with acceptable diagnostic capacity. The results of the functional enrichment analysis indicated that these identified marker genes may be involved in the pathogenesis of BPD through the regulation of immune response, cell cycle, and BPD-related pathways. Additionally, we identified 29 drugs that target 5 of the marker genes, which could have potential therapeutic implications. The ceRNA network we constructed revealed a complex regulatory network based on the marker genes, further highlighting their potential roles in BPD. Our findings offer diagnostic potential and insight into the mechanism underlying BPD. Further research is needed to assess its clinical utility.
format Online
Article
Text
id pubmed-10662800
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Lippincott Williams & Wilkins
record_format MEDLINE/PubMed
spelling pubmed-106628002023-07-21 Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia Ma, Xiaoxue Tao, Ziyu Chen, Leiming Duan, Shaozhi Zhou, Guoping Ma, Yunxia Xiong, Zhenqin Zhu, Lan Ma, Xuejiao Mao, Yan Hu, Yifang Zeng, Ni Wang, Jimei Bao, Yunlei Luo, Fei Wu, Chuyan Jiang, Feng Medicine (Baltimore) 6200 Ferroptosis is a recently identified form of cell death that is distinct from the conventional modes such as necrosis, apoptosis, and autophagy. Its role in bronchopulmonary dysplasia (BPD) remains inadequately understood. To address this gap, we obtained BPD-related RNA-seq data and ferroptosis-related genes (FRGs) from the GEO database and FerrDb, respectively. A total of 171 BPD-related differentially expressed ferroptosis-related genes (DE-FRGs) linked to the regulation of autophagy and immune response were identified. Least absolute shrinkage and selection operator and SVM-RFE algorithms identified 23 and 14 genes, respectively, as marker genes. The intersection of these 2 sets yielded 9 genes (ALOX12B, NR1D1, LGMN, IFNA21, MEG3, AKR1C1, CA9, ABCC5, and GALNT14) with acceptable diagnostic capacity. The results of the functional enrichment analysis indicated that these identified marker genes may be involved in the pathogenesis of BPD through the regulation of immune response, cell cycle, and BPD-related pathways. Additionally, we identified 29 drugs that target 5 of the marker genes, which could have potential therapeutic implications. The ceRNA network we constructed revealed a complex regulatory network based on the marker genes, further highlighting their potential roles in BPD. Our findings offer diagnostic potential and insight into the mechanism underlying BPD. Further research is needed to assess its clinical utility. Lippincott Williams & Wilkins 2023-07-21 /pmc/articles/PMC10662800/ /pubmed/37478211 http://dx.doi.org/10.1097/MD.0000000000034371 Text en Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle 6200
Ma, Xiaoxue
Tao, Ziyu
Chen, Leiming
Duan, Shaozhi
Zhou, Guoping
Ma, Yunxia
Xiong, Zhenqin
Zhu, Lan
Ma, Xuejiao
Mao, Yan
Hu, Yifang
Zeng, Ni
Wang, Jimei
Bao, Yunlei
Luo, Fei
Wu, Chuyan
Jiang, Feng
Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia
title Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia
title_full Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia
title_fullStr Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia
title_full_unstemmed Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia
title_short Genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia
title_sort genetic analysis of potential biomarkers and therapeutic targets associated with ferroptosis from bronchopulmonary dysplasia
topic 6200
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662800/
https://www.ncbi.nlm.nih.gov/pubmed/37478211
http://dx.doi.org/10.1097/MD.0000000000034371
work_keys_str_mv AT maxiaoxue geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT taoziyu geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT chenleiming geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT duanshaozhi geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT zhouguoping geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT mayunxia geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT xiongzhenqin geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT zhulan geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT maxuejiao geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT maoyan geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT huyifang geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT zengni geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT wangjimei geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT baoyunlei geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT luofei geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT wuchuyan geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia
AT jiangfeng geneticanalysisofpotentialbiomarkersandtherapeutictargetsassociatedwithferroptosisfrombronchopulmonarydysplasia