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Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children
This study aimed to evaluate the diagnostic value of chemiluminescence immunoassay (CLIA), passive particle agglutination (PPA), and indirect immunofluorescence assay (IFA) in detecting Mycoplasma pneumoniae infection in children. Serum samples from 165 children with acute community-acquired respira...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662900/ https://www.ncbi.nlm.nih.gov/pubmed/37478238 http://dx.doi.org/10.1097/MD.0000000000034133 |
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author | Liu, Mengyang Meng, Ke Jiang, Jun Zhang, Li Sun, Shiying |
author_facet | Liu, Mengyang Meng, Ke Jiang, Jun Zhang, Li Sun, Shiying |
author_sort | Liu, Mengyang |
collection | PubMed |
description | This study aimed to evaluate the diagnostic value of chemiluminescence immunoassay (CLIA), passive particle agglutination (PPA), and indirect immunofluorescence assay (IFA) in detecting Mycoplasma pneumoniae infection in children. Serum samples from 165 children with acute community-acquired respiratory tract infections were examined using CLIA, PPA, and IFA, and consistency coefficient, specificity, and sensitivity were analyzed. Compared with the PPA (titer ≥ 1:160), the consistency coefficients of the immunoglobulin(Ig)M-CLIA, immunoglobulin(Ig)G-CLIA and IgM-IFA methods were 93.94%, 75.76%, and 83.64%, respectively. The positive likelihood ratio (PLR) and specificity of IgM-CLIA was 19.40 and 95.49%, respectively. The consistency coefficient of (IgM+IgG)-CLIA and PPA (titer ≥ 1:160) was 89.1%, and the sensitivity and negative predictive value of (IgM+IgG)-CLIA were 96.88% and 98.94%, respectively. CLIA MP-IgM has high concordance with PPA, and its specificity and sensitivity are higher than those of CLIA MP-IgG and IFA MP-IgM, suggesting its better diagnosis of early MP infection. The sensitivity and negative predictive value of CLIA MP (IgM+IgG) were higher than those of PPA or IFA, indicating that it should be considered as a priority in the diagnosis of MP infection. |
format | Online Article Text |
id | pubmed-10662900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-106629002023-07-21 Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children Liu, Mengyang Meng, Ke Jiang, Jun Zhang, Li Sun, Shiying Medicine (Baltimore) 4900 This study aimed to evaluate the diagnostic value of chemiluminescence immunoassay (CLIA), passive particle agglutination (PPA), and indirect immunofluorescence assay (IFA) in detecting Mycoplasma pneumoniae infection in children. Serum samples from 165 children with acute community-acquired respiratory tract infections were examined using CLIA, PPA, and IFA, and consistency coefficient, specificity, and sensitivity were analyzed. Compared with the PPA (titer ≥ 1:160), the consistency coefficients of the immunoglobulin(Ig)M-CLIA, immunoglobulin(Ig)G-CLIA and IgM-IFA methods were 93.94%, 75.76%, and 83.64%, respectively. The positive likelihood ratio (PLR) and specificity of IgM-CLIA was 19.40 and 95.49%, respectively. The consistency coefficient of (IgM+IgG)-CLIA and PPA (titer ≥ 1:160) was 89.1%, and the sensitivity and negative predictive value of (IgM+IgG)-CLIA were 96.88% and 98.94%, respectively. CLIA MP-IgM has high concordance with PPA, and its specificity and sensitivity are higher than those of CLIA MP-IgG and IFA MP-IgM, suggesting its better diagnosis of early MP infection. The sensitivity and negative predictive value of CLIA MP (IgM+IgG) were higher than those of PPA or IFA, indicating that it should be considered as a priority in the diagnosis of MP infection. Lippincott Williams & Wilkins 2023-07-21 /pmc/articles/PMC10662900/ /pubmed/37478238 http://dx.doi.org/10.1097/MD.0000000000034133 Text en Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | 4900 Liu, Mengyang Meng, Ke Jiang, Jun Zhang, Li Sun, Shiying Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children |
title | Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children |
title_full | Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children |
title_fullStr | Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children |
title_full_unstemmed | Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children |
title_short | Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children |
title_sort | comparison of serodiagnosis methods for community-acquired mycoplasma pneumoniae respiratory tract infections in children |
topic | 4900 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10662900/ https://www.ncbi.nlm.nih.gov/pubmed/37478238 http://dx.doi.org/10.1097/MD.0000000000034133 |
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