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x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light
Plants cope with sudden increases in light intensity through various photoprotective mechanisms. Redox regulation by thioredoxin (Trx) systems also contributes to this process. Whereas the functions of f- and m-type Trxs in response to such fluctuating light conditions have been extensively investig...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663110/ https://www.ncbi.nlm.nih.gov/pubmed/37606239 http://dx.doi.org/10.1093/plphys/kiad466 |
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author | Okegawa, Yuki Sato, Nozomi Nakakura, Rino Murai, Ryota Sakamoto, Wataru Motohashi, Ken |
author_facet | Okegawa, Yuki Sato, Nozomi Nakakura, Rino Murai, Ryota Sakamoto, Wataru Motohashi, Ken |
author_sort | Okegawa, Yuki |
collection | PubMed |
description | Plants cope with sudden increases in light intensity through various photoprotective mechanisms. Redox regulation by thioredoxin (Trx) systems also contributes to this process. Whereas the functions of f- and m-type Trxs in response to such fluctuating light conditions have been extensively investigated, those of x- and y-type Trxs are largely unknown. Here, we analyzed the trx x single, trx y1 trx y2 double, and trx x trx y1 trx y2 triple mutants in Arabidopsis (Arabidopsis thaliana). A detailed analysis of photosynthesis revealed changes in photosystem I (PSI) parameters under low light in trx x and trx x trx y1 trx y2. The electron acceptor side of PSI was more reduced in these mutants than in the wild type. This mutant phenotype was more pronounced under fluctuating light conditions. During both low- and high-light phases, the PSI acceptor side was largely limited in trx x and trx x trx y1 trx y2. After fluctuating light treatment, we observed more severe PSI photoinhibition in trx x and trx x trx y1 trx y2 than in the wild type. Furthermore, when grown under fluctuating light conditions, trx x and trx x trx y1 trx y2 plants showed impaired growth and decreased level of PSI subunits. These results suggest that Trx x and Trx y prevent redox imbalance on the PSI acceptor side, which is required to protect PSI from photoinhibition, especially under fluctuating light. We also propose that Trx x and Trx y contribute to maintaining the redox balance even under constant low-light conditions to prepare for sudden increases in light intensity. |
format | Online Article Text |
id | pubmed-10663110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-106631102023-08-22 x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light Okegawa, Yuki Sato, Nozomi Nakakura, Rino Murai, Ryota Sakamoto, Wataru Motohashi, Ken Plant Physiol Research Article Plants cope with sudden increases in light intensity through various photoprotective mechanisms. Redox regulation by thioredoxin (Trx) systems also contributes to this process. Whereas the functions of f- and m-type Trxs in response to such fluctuating light conditions have been extensively investigated, those of x- and y-type Trxs are largely unknown. Here, we analyzed the trx x single, trx y1 trx y2 double, and trx x trx y1 trx y2 triple mutants in Arabidopsis (Arabidopsis thaliana). A detailed analysis of photosynthesis revealed changes in photosystem I (PSI) parameters under low light in trx x and trx x trx y1 trx y2. The electron acceptor side of PSI was more reduced in these mutants than in the wild type. This mutant phenotype was more pronounced under fluctuating light conditions. During both low- and high-light phases, the PSI acceptor side was largely limited in trx x and trx x trx y1 trx y2. After fluctuating light treatment, we observed more severe PSI photoinhibition in trx x and trx x trx y1 trx y2 than in the wild type. Furthermore, when grown under fluctuating light conditions, trx x and trx x trx y1 trx y2 plants showed impaired growth and decreased level of PSI subunits. These results suggest that Trx x and Trx y prevent redox imbalance on the PSI acceptor side, which is required to protect PSI from photoinhibition, especially under fluctuating light. We also propose that Trx x and Trx y contribute to maintaining the redox balance even under constant low-light conditions to prepare for sudden increases in light intensity. Oxford University Press 2023-08-22 /pmc/articles/PMC10663110/ /pubmed/37606239 http://dx.doi.org/10.1093/plphys/kiad466 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Okegawa, Yuki Sato, Nozomi Nakakura, Rino Murai, Ryota Sakamoto, Wataru Motohashi, Ken x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light |
title |
x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light |
title_full |
x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light |
title_fullStr |
x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light |
title_full_unstemmed |
x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light |
title_short |
x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light |
title_sort | x- and y-type thioredoxins maintain redox homeostasis on photosystem i acceptor side under fluctuating light |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663110/ https://www.ncbi.nlm.nih.gov/pubmed/37606239 http://dx.doi.org/10.1093/plphys/kiad466 |
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