BK channels sustain neuronal Ca(2+) oscillations to support hippocampal long-term potentiation and memory formation

Mutations of large conductance Ca(2+)- and voltage-activated K(+) channels (BK) are associated with cognitive impairment. Here we report that CA1 pyramidal neuron-specific conditional BK knock-out (cKO) mice display normal locomotor and anxiety behavior. They do, however, exhibit impaired memory acquisition and retrieval in the Morris Water Maze (MWM) when compared to littermate controls (CTRL). In line with cognitive impairment in vivo, electrical and chemical long-term potentiation (LTP) in cKO brain slices were impaired in vitro. We further used a genetically encoded fluorescent K(+) biosensor and a Ca(2+)-sensitive probe to observe cultured hippocampal neurons during chemical LTP (cLTP) induction. cLTP massively reduced intracellular K(+) concentration ([K(+)](i)) while elevating L-Type Ca(2+) channel- and NMDA receptor-dependent Ca(2+) oscillation frequencies. Both, [K(+)](i) decrease and Ca(2+) oscillation frequency increase were absent after pharmacological BK inhibition or in cells lacking BK. Our data suggest that L-Type- and NMDAR-dependent BK-mediated K(+) outflow significantly contributes to hippocampal LTP, as well as learning and memory. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00018-023-05016-y.