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Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing
Neuroblastoma (NB) is an embryonic tumour that originates in the sympathetic nervous system and occurs most often in infants and children under 2 years of age. Moreover, it is the most common extracranial solid tumour in children. Increasing studies suggest that intercellular communication within th...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663476/ https://www.ncbi.nlm.nih.gov/pubmed/37990103 http://dx.doi.org/10.1038/s41598-023-47796-0 |
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author | Chu, Jing |
author_facet | Chu, Jing |
author_sort | Chu, Jing |
collection | PubMed |
description | Neuroblastoma (NB) is an embryonic tumour that originates in the sympathetic nervous system and occurs most often in infants and children under 2 years of age. Moreover, it is the most common extracranial solid tumour in children. Increasing studies suggest that intercellular communication within the tumour microenvironment is closely related to tumour development. This study aimed to construct a prognosis-related intercellular communication-associated genes model by single-cell sequencing and transcriptome sequencing to predict the prognosis of patients with NB for precise management. Single-cell data from patients with NB were downloaded from the gene expression omnibus database for comprehensive analysis. Furthermore, prognosis-related genes were screened in the TARGET database based on epithelial cell marker genes through a combination of Cox regression and Lasso regression analyses, using GSE62564 and GSE85047 for external validation. The patients’ risk scores were calculated, followed by immune infiltration analysis, drug sensitivity analysis, and enrichment analysis of risk scores, which were conducted for the prognostic model. I used the Lasso regression feature selection algorithm to screen characteristic genes in NB and developed a 21-gene prognostic model. The risk scores were highly correlated with multiple immune cells and common anti-tumour drugs. Furthermore, the risk score was identified as an independent prognostic factor for NB. In this study, I constructed and validated a prognostic signature based on epithelial marker genes, which may provide useful information on the development and prognosis of NB. |
format | Online Article Text |
id | pubmed-10663476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106634762023-11-21 Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing Chu, Jing Sci Rep Article Neuroblastoma (NB) is an embryonic tumour that originates in the sympathetic nervous system and occurs most often in infants and children under 2 years of age. Moreover, it is the most common extracranial solid tumour in children. Increasing studies suggest that intercellular communication within the tumour microenvironment is closely related to tumour development. This study aimed to construct a prognosis-related intercellular communication-associated genes model by single-cell sequencing and transcriptome sequencing to predict the prognosis of patients with NB for precise management. Single-cell data from patients with NB were downloaded from the gene expression omnibus database for comprehensive analysis. Furthermore, prognosis-related genes were screened in the TARGET database based on epithelial cell marker genes through a combination of Cox regression and Lasso regression analyses, using GSE62564 and GSE85047 for external validation. The patients’ risk scores were calculated, followed by immune infiltration analysis, drug sensitivity analysis, and enrichment analysis of risk scores, which were conducted for the prognostic model. I used the Lasso regression feature selection algorithm to screen characteristic genes in NB and developed a 21-gene prognostic model. The risk scores were highly correlated with multiple immune cells and common anti-tumour drugs. Furthermore, the risk score was identified as an independent prognostic factor for NB. In this study, I constructed and validated a prognostic signature based on epithelial marker genes, which may provide useful information on the development and prognosis of NB. Nature Publishing Group UK 2023-11-21 /pmc/articles/PMC10663476/ /pubmed/37990103 http://dx.doi.org/10.1038/s41598-023-47796-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Chu, Jing Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing |
title | Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing |
title_full | Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing |
title_fullStr | Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing |
title_full_unstemmed | Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing |
title_short | Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing |
title_sort | exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663476/ https://www.ncbi.nlm.nih.gov/pubmed/37990103 http://dx.doi.org/10.1038/s41598-023-47796-0 |
work_keys_str_mv | AT chujing explorationofthemolecularmechanismofintercellularcommunicationinpaediatricneuroblastomabysinglecellsequencing |