AePUb promoter length modulates gene expression in Aedes aegypti

Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasin...

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Autores principales: Anderson, Michelle A. E., Leftwich, Philip T., Wilson, Ray, Paladino, Leonela Z. Carabajal, Basu, Sanjay, Rooney, Sara, Adelman, Zach N., Alphey, Luke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663553/
https://www.ncbi.nlm.nih.gov/pubmed/37990055
http://dx.doi.org/10.1038/s41598-023-47777-3
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author Anderson, Michelle A. E.
Leftwich, Philip T.
Wilson, Ray
Paladino, Leonela Z. Carabajal
Basu, Sanjay
Rooney, Sara
Adelman, Zach N.
Alphey, Luke
author_facet Anderson, Michelle A. E.
Leftwich, Philip T.
Wilson, Ray
Paladino, Leonela Z. Carabajal
Basu, Sanjay
Rooney, Sara
Adelman, Zach N.
Alphey, Luke
author_sort Anderson, Michelle A. E.
collection PubMed
description Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
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spelling pubmed-106635532023-11-21 AePUb promoter length modulates gene expression in Aedes aegypti Anderson, Michelle A. E. Leftwich, Philip T. Wilson, Ray Paladino, Leonela Z. Carabajal Basu, Sanjay Rooney, Sara Adelman, Zach N. Alphey, Luke Sci Rep Article Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects. Nature Publishing Group UK 2023-11-21 /pmc/articles/PMC10663553/ /pubmed/37990055 http://dx.doi.org/10.1038/s41598-023-47777-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Anderson, Michelle A. E.
Leftwich, Philip T.
Wilson, Ray
Paladino, Leonela Z. Carabajal
Basu, Sanjay
Rooney, Sara
Adelman, Zach N.
Alphey, Luke
AePUb promoter length modulates gene expression in Aedes aegypti
title AePUb promoter length modulates gene expression in Aedes aegypti
title_full AePUb promoter length modulates gene expression in Aedes aegypti
title_fullStr AePUb promoter length modulates gene expression in Aedes aegypti
title_full_unstemmed AePUb promoter length modulates gene expression in Aedes aegypti
title_short AePUb promoter length modulates gene expression in Aedes aegypti
title_sort aepub promoter length modulates gene expression in aedes aegypti
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663553/
https://www.ncbi.nlm.nih.gov/pubmed/37990055
http://dx.doi.org/10.1038/s41598-023-47777-3
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