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Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L.
Selenium, an essential micronutrient for plants and animals, can cause selenium toxicity as an oxyanion or at elevated doses. However, the toxic selenite (SeO(3)(2−)) oxyanion, can be converted into less harmful elemental nano-selenium (Se(0)), with various practical applications. This research aime...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663618/ https://www.ncbi.nlm.nih.gov/pubmed/37989844 http://dx.doi.org/10.1038/s41598-023-47616-5 |
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author | Tendenedzai, Job T. Chirwa, Evans M. N. Brink, Hendrik G. |
author_facet | Tendenedzai, Job T. Chirwa, Evans M. N. Brink, Hendrik G. |
author_sort | Tendenedzai, Job T. |
collection | PubMed |
description | Selenium, an essential micronutrient for plants and animals, can cause selenium toxicity as an oxyanion or at elevated doses. However, the toxic selenite (SeO(3)(2−)) oxyanion, can be converted into less harmful elemental nano-selenium (Se(0)), with various practical applications. This research aimed to investigate two methods for reducing SeO(3)(2−): abiotic reduction using cell-free extract from Enterococcus spp. (abiotic-SeNPs) and chemical reduction involving L-ascorbic acid (chemical-SeNPs). Analysis with XPS confirmed the presence of Se(0), while FTIR analysis identified surface functional groups on all SeNPs. The study evaluated the effects of SeO(3)(2−), abiotic-SeNPs, and chemical-SeNPs at different concentrations on the growth and germination of Pisum sativum L. seeds. SeO(3)(2−) demonstrated detrimental effects on germination at concentrations of 1 ppm (germination index (GI) = 0.3). Conversely, both abiotic- and chemical-SeNPs had positive impacts on germination, with GI > 120 at 10 ppm. Through the DPPH assay, it was discovered that SeNPs exhibited superior antioxidant capabilities at 80 ppm, achieving over 70% inhibition, compared to SeO(3)(2−) (less than 20% inhibition), therefore evidencing significant antioxidant properties. This demonstrates that SeNPs have the potential to be utilized as an agricultural fertilizer additive, benefiting seedling germination and development, while also protecting against oxidative stress. |
format | Online Article Text |
id | pubmed-10663618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106636182023-11-21 Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L. Tendenedzai, Job T. Chirwa, Evans M. N. Brink, Hendrik G. Sci Rep Article Selenium, an essential micronutrient for plants and animals, can cause selenium toxicity as an oxyanion or at elevated doses. However, the toxic selenite (SeO(3)(2−)) oxyanion, can be converted into less harmful elemental nano-selenium (Se(0)), with various practical applications. This research aimed to investigate two methods for reducing SeO(3)(2−): abiotic reduction using cell-free extract from Enterococcus spp. (abiotic-SeNPs) and chemical reduction involving L-ascorbic acid (chemical-SeNPs). Analysis with XPS confirmed the presence of Se(0), while FTIR analysis identified surface functional groups on all SeNPs. The study evaluated the effects of SeO(3)(2−), abiotic-SeNPs, and chemical-SeNPs at different concentrations on the growth and germination of Pisum sativum L. seeds. SeO(3)(2−) demonstrated detrimental effects on germination at concentrations of 1 ppm (germination index (GI) = 0.3). Conversely, both abiotic- and chemical-SeNPs had positive impacts on germination, with GI > 120 at 10 ppm. Through the DPPH assay, it was discovered that SeNPs exhibited superior antioxidant capabilities at 80 ppm, achieving over 70% inhibition, compared to SeO(3)(2−) (less than 20% inhibition), therefore evidencing significant antioxidant properties. This demonstrates that SeNPs have the potential to be utilized as an agricultural fertilizer additive, benefiting seedling germination and development, while also protecting against oxidative stress. Nature Publishing Group UK 2023-11-21 /pmc/articles/PMC10663618/ /pubmed/37989844 http://dx.doi.org/10.1038/s41598-023-47616-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Tendenedzai, Job T. Chirwa, Evans M. N. Brink, Hendrik G. Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L. |
title | Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L. |
title_full | Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L. |
title_fullStr | Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L. |
title_full_unstemmed | Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L. |
title_short | Harnessing selenium nanoparticles (SeNPs) for enhancing growth and germination, and mitigating oxidative stress in Pisum sativum L. |
title_sort | harnessing selenium nanoparticles (senps) for enhancing growth and germination, and mitigating oxidative stress in pisum sativum l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663618/ https://www.ncbi.nlm.nih.gov/pubmed/37989844 http://dx.doi.org/10.1038/s41598-023-47616-5 |
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