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Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma

Cellular crosstalk in the tumor microenvironment (TME) is still largely uncharacterized, while it plays an essential role in shaping immunosuppression or anti-tumor response. Large-scale analyses are needed to better decipher cell-cell communication in cancer. In this work, we used original and publ...

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Autores principales: Massenet-Regad, Lucile, Poirot, Justine, Jackson, Margaret, Hoffmann, Caroline, Amblard, Elise, Onodi, Fanny, Bouhidel, Fatiha, Djouadou, Malika, Ouzaid, Idir, Xylinas, Evanguelos, Medvedovic, Jasna, Soumelis, Vassili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663819/
https://www.ncbi.nlm.nih.gov/pubmed/38025776
http://dx.doi.org/10.1016/j.isci.2023.108367
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author Massenet-Regad, Lucile
Poirot, Justine
Jackson, Margaret
Hoffmann, Caroline
Amblard, Elise
Onodi, Fanny
Bouhidel, Fatiha
Djouadou, Malika
Ouzaid, Idir
Xylinas, Evanguelos
Medvedovic, Jasna
Soumelis, Vassili
author_facet Massenet-Regad, Lucile
Poirot, Justine
Jackson, Margaret
Hoffmann, Caroline
Amblard, Elise
Onodi, Fanny
Bouhidel, Fatiha
Djouadou, Malika
Ouzaid, Idir
Xylinas, Evanguelos
Medvedovic, Jasna
Soumelis, Vassili
author_sort Massenet-Regad, Lucile
collection PubMed
description Cellular crosstalk in the tumor microenvironment (TME) is still largely uncharacterized, while it plays an essential role in shaping immunosuppression or anti-tumor response. Large-scale analyses are needed to better decipher cell-cell communication in cancer. In this work, we used original and publicly available single-cell RNA sequencing (scRNAseq) data to characterize in-depth the communication networks in human clear cell renal cell carcinoma (ccRCC). We identified 50 putative communication channels specifically used by cancer cells to interact with other cells, including two novel angiogenin-mediated interactions. Expression of angiogenin and its receptors was validated at the protein level in primary ccRCC. Mechanistically, angiogenin enhanced ccRCC cell line proliferation and down-regulated secretion of IL-6, IL-8, and MCP-1 proinflammatory molecules. This study provides novel biological insights into molecular mechanisms of ccRCC, and suggests angiogenin and its receptors as potential therapeutic targets in clear cell renal cancer.
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spelling pubmed-106638192023-10-31 Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma Massenet-Regad, Lucile Poirot, Justine Jackson, Margaret Hoffmann, Caroline Amblard, Elise Onodi, Fanny Bouhidel, Fatiha Djouadou, Malika Ouzaid, Idir Xylinas, Evanguelos Medvedovic, Jasna Soumelis, Vassili iScience Article Cellular crosstalk in the tumor microenvironment (TME) is still largely uncharacterized, while it plays an essential role in shaping immunosuppression or anti-tumor response. Large-scale analyses are needed to better decipher cell-cell communication in cancer. In this work, we used original and publicly available single-cell RNA sequencing (scRNAseq) data to characterize in-depth the communication networks in human clear cell renal cell carcinoma (ccRCC). We identified 50 putative communication channels specifically used by cancer cells to interact with other cells, including two novel angiogenin-mediated interactions. Expression of angiogenin and its receptors was validated at the protein level in primary ccRCC. Mechanistically, angiogenin enhanced ccRCC cell line proliferation and down-regulated secretion of IL-6, IL-8, and MCP-1 proinflammatory molecules. This study provides novel biological insights into molecular mechanisms of ccRCC, and suggests angiogenin and its receptors as potential therapeutic targets in clear cell renal cancer. Elsevier 2023-10-31 /pmc/articles/PMC10663819/ /pubmed/38025776 http://dx.doi.org/10.1016/j.isci.2023.108367 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Massenet-Regad, Lucile
Poirot, Justine
Jackson, Margaret
Hoffmann, Caroline
Amblard, Elise
Onodi, Fanny
Bouhidel, Fatiha
Djouadou, Malika
Ouzaid, Idir
Xylinas, Evanguelos
Medvedovic, Jasna
Soumelis, Vassili
Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma
title Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma
title_full Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma
title_fullStr Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma
title_full_unstemmed Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma
title_short Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma
title_sort large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663819/
https://www.ncbi.nlm.nih.gov/pubmed/38025776
http://dx.doi.org/10.1016/j.isci.2023.108367
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