Protocol for performing and optimizing differential scanning fluorimetry experiments

Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing and optimizing DSF experiments. We describe steps for designing and performing the experiment, analyzing data, a...

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Detalles Bibliográficos
Autores principales: Wu, Taiasean, Hornsby, Michael, Zhu, Lawrence, Yu, Joshua C., Shokat, Kevan M., Gestwicki, Jason E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663957/
https://www.ncbi.nlm.nih.gov/pubmed/37943662
http://dx.doi.org/10.1016/j.xpro.2023.102688

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663957/
https://www.ncbi.nlm.nih.gov/pubmed/37943662
http://dx.doi.org/10.1016/j.xpro.2023.102688

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