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Protocol for isolating mice skeletal muscle myoblasts and myotubes via differential antibody validation

Isolation of skeletal muscles allows for the exploration of many complex diseases. Here, we present a protocol for isolating mice skeletal muscle myoblasts and myotubes that have been differentiated through antibody validation. We describe steps for collecting and preparing murine skeletal tissue, m...

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Detalles Bibliográficos
Autores principales: Stephens, Dominique C., Mungai, Margaret, Crabtree, Amber, Beasley, Heather K., Garza-Lopez, Edgar, Vang, Larry, Neikirk, Kit, Vue, Zer, Vue, Neng, Marshall, Andrea G., Turner, Kyrin, Shao, Jian-qiang, Sarker, Bishnu, Murray, Sandra, Gaddy, Jennifer A., Davis, Jamaine, Damo, Steven M., Hinton, Antentor O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10663959/
https://www.ncbi.nlm.nih.gov/pubmed/37938976
http://dx.doi.org/10.1016/j.xpro.2023.102591