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Flash NanoPrecipitation as an Agrochemical Nanocarrier Formulation Platform: Phloem Uptake and Translocation after Foliar Administration
[Image: see text] The increasing severity of pathogenic and environmental stressors that negatively affect plant health has led to interest in developing next-generation agrochemical delivery systems capable of precisely transporting active agents to specific sites within plants. In this work, we ad...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10664067/ https://www.ncbi.nlm.nih.gov/pubmed/38021209 http://dx.doi.org/10.1021/acsagscitech.3c00204 |
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author | Ristroph, Kurt Zhang, Yilin Nava, Valeria Wielinski, Jonas Kohay, Hagay Kiss, Andrew M. Thieme, Juergen Lowry, Gregory V. |
author_facet | Ristroph, Kurt Zhang, Yilin Nava, Valeria Wielinski, Jonas Kohay, Hagay Kiss, Andrew M. Thieme, Juergen Lowry, Gregory V. |
author_sort | Ristroph, Kurt |
collection | PubMed |
description | [Image: see text] The increasing severity of pathogenic and environmental stressors that negatively affect plant health has led to interest in developing next-generation agrochemical delivery systems capable of precisely transporting active agents to specific sites within plants. In this work, we adapt Flash NanoPrecipitation (FNP), a scalable nanocarrier (NC) formulation technology used in the pharmaceutical industry, to prepare organic core–shell NCs and study their efficacy as foliar or root delivery vehicles. NCs ranging in diameter from 55 to 200 nm, with surface zeta potentials from −40 to +40 mV, and with seven different shell material properties were prepared and studied. Shell materials included synthetic polymers poly(acrylic acid), poly(ethylene glycol), and poly(2-(dimethylamino)ethyl methacrylate), naturally occurring compounds fish gelatin and soybean lecithin, and semisynthetic hydroxypropyl methylcellulose acetate succinate (HPMCAS). NC cores contained a gadolinium tracer for tracking by mass spectrometry, a fluorescent dye for tracking by confocal microscopy, and model hydrophobic compounds (alpha tocopherol acetate and polystyrene) that could be replaced by agrochemical payloads in subsequent applications. After foliar application onto tomato plants with Silwet L-77 surfactant, internalization efficiencies of up to 85% and NC translocation efficiencies of up to 32% were observed. Significant NC trafficking to the stem and roots suggests a high degree of phloem loading for some of these formulations. Results were corroborated by confocal microscopy and synchrotron X-ray fluorescence mapping. NCs stabilized by cellulosic HPMCAS exhibited the highest degree of translocation, followed by formulations with a significant surface charge. The results from this work indicate that biocompatible materials like HPMCAS are promising agrochemical delivery vehicles in an industrially viable pharmaceutical nanoformulation process (FNP) and shed light on the optimal properties of organic NCs for efficient foliar uptake, translocation, and delivery. |
format | Online Article Text |
id | pubmed-10664067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-106640672023-11-22 Flash NanoPrecipitation as an Agrochemical Nanocarrier Formulation Platform: Phloem Uptake and Translocation after Foliar Administration Ristroph, Kurt Zhang, Yilin Nava, Valeria Wielinski, Jonas Kohay, Hagay Kiss, Andrew M. Thieme, Juergen Lowry, Gregory V. ACS Agric Sci Technol [Image: see text] The increasing severity of pathogenic and environmental stressors that negatively affect plant health has led to interest in developing next-generation agrochemical delivery systems capable of precisely transporting active agents to specific sites within plants. In this work, we adapt Flash NanoPrecipitation (FNP), a scalable nanocarrier (NC) formulation technology used in the pharmaceutical industry, to prepare organic core–shell NCs and study their efficacy as foliar or root delivery vehicles. NCs ranging in diameter from 55 to 200 nm, with surface zeta potentials from −40 to +40 mV, and with seven different shell material properties were prepared and studied. Shell materials included synthetic polymers poly(acrylic acid), poly(ethylene glycol), and poly(2-(dimethylamino)ethyl methacrylate), naturally occurring compounds fish gelatin and soybean lecithin, and semisynthetic hydroxypropyl methylcellulose acetate succinate (HPMCAS). NC cores contained a gadolinium tracer for tracking by mass spectrometry, a fluorescent dye for tracking by confocal microscopy, and model hydrophobic compounds (alpha tocopherol acetate and polystyrene) that could be replaced by agrochemical payloads in subsequent applications. After foliar application onto tomato plants with Silwet L-77 surfactant, internalization efficiencies of up to 85% and NC translocation efficiencies of up to 32% were observed. Significant NC trafficking to the stem and roots suggests a high degree of phloem loading for some of these formulations. Results were corroborated by confocal microscopy and synchrotron X-ray fluorescence mapping. NCs stabilized by cellulosic HPMCAS exhibited the highest degree of translocation, followed by formulations with a significant surface charge. The results from this work indicate that biocompatible materials like HPMCAS are promising agrochemical delivery vehicles in an industrially viable pharmaceutical nanoformulation process (FNP) and shed light on the optimal properties of organic NCs for efficient foliar uptake, translocation, and delivery. American Chemical Society 2023-10-17 /pmc/articles/PMC10664067/ /pubmed/38021209 http://dx.doi.org/10.1021/acsagscitech.3c00204 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Ristroph, Kurt Zhang, Yilin Nava, Valeria Wielinski, Jonas Kohay, Hagay Kiss, Andrew M. Thieme, Juergen Lowry, Gregory V. Flash NanoPrecipitation as an Agrochemical Nanocarrier Formulation Platform: Phloem Uptake and Translocation after Foliar Administration |
title | Flash NanoPrecipitation
as an Agrochemical Nanocarrier
Formulation Platform: Phloem Uptake and Translocation after Foliar
Administration |
title_full | Flash NanoPrecipitation
as an Agrochemical Nanocarrier
Formulation Platform: Phloem Uptake and Translocation after Foliar
Administration |
title_fullStr | Flash NanoPrecipitation
as an Agrochemical Nanocarrier
Formulation Platform: Phloem Uptake and Translocation after Foliar
Administration |
title_full_unstemmed | Flash NanoPrecipitation
as an Agrochemical Nanocarrier
Formulation Platform: Phloem Uptake and Translocation after Foliar
Administration |
title_short | Flash NanoPrecipitation
as an Agrochemical Nanocarrier
Formulation Platform: Phloem Uptake and Translocation after Foliar
Administration |
title_sort | flash nanoprecipitation
as an agrochemical nanocarrier
formulation platform: phloem uptake and translocation after foliar
administration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10664067/ https://www.ncbi.nlm.nih.gov/pubmed/38021209 http://dx.doi.org/10.1021/acsagscitech.3c00204 |
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