Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin

BACKGROUND: Diabetic nephropathy (DN) represents a microvascular complication of diabetes mellitus (DM). Despite the increasing incidence and prevalence of DN, conservative therapy only reduces risk factors and hemodialysis. This research aimed at finding DN animal model that can be tried to be give...

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Autores principales: Wahono, Aditya Mahardika, Harnanik, Titut, Pasaribu, Irma A., Adiwinoto, Ronald Pratama, Octavianda, Yohana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10664383/
https://www.ncbi.nlm.nih.gov/pubmed/37990213
http://dx.doi.org/10.1186/s12902-023-01504-1
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author Wahono, Aditya Mahardika
Harnanik, Titut
Pasaribu, Irma A.
Adiwinoto, Ronald Pratama
Octavianda, Yohana
author_facet Wahono, Aditya Mahardika
Harnanik, Titut
Pasaribu, Irma A.
Adiwinoto, Ronald Pratama
Octavianda, Yohana
author_sort Wahono, Aditya Mahardika
collection PubMed
description BACKGROUND: Diabetic nephropathy (DN) represents a microvascular complication of diabetes mellitus (DM). Despite the increasing incidence and prevalence of DN, conservative therapy only reduces risk factors and hemodialysis. This research aimed at finding DN animal model that can be tried to be given an alternative treatment. DN was assessed by evaluating body weight, blood glucose, proteinuria, and kidney histopathology. METHODS: Wistar novergicus male rats were induced with 75 mg of streptozotocin per kg BW to obtain a diabetic nephropathy model. The 18 rats were divided into 2 groups consisting of 9 rats in the negative group (G0) and 9 rats in the positive group (G1). Indicators of body weight, blood glucose levels, urine protein and kidney histopathology determine the incidents of DN animal models. RESULT: Rats induced using 75 mg of streptozotocin per kg body weight (BW) indicated weight loss, increased blood glucose, urine protein levels and histopathological features of DN. CONCLUSION: Seventy-five mg of streptozotocin per kg BW can induce a diabetic nephropathy animal model in Wistar norvegicus rats. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12902-023-01504-1.
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spelling pubmed-106643832023-11-22 Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin Wahono, Aditya Mahardika Harnanik, Titut Pasaribu, Irma A. Adiwinoto, Ronald Pratama Octavianda, Yohana BMC Endocr Disord Research BACKGROUND: Diabetic nephropathy (DN) represents a microvascular complication of diabetes mellitus (DM). Despite the increasing incidence and prevalence of DN, conservative therapy only reduces risk factors and hemodialysis. This research aimed at finding DN animal model that can be tried to be given an alternative treatment. DN was assessed by evaluating body weight, blood glucose, proteinuria, and kidney histopathology. METHODS: Wistar novergicus male rats were induced with 75 mg of streptozotocin per kg BW to obtain a diabetic nephropathy model. The 18 rats were divided into 2 groups consisting of 9 rats in the negative group (G0) and 9 rats in the positive group (G1). Indicators of body weight, blood glucose levels, urine protein and kidney histopathology determine the incidents of DN animal models. RESULT: Rats induced using 75 mg of streptozotocin per kg body weight (BW) indicated weight loss, increased blood glucose, urine protein levels and histopathological features of DN. CONCLUSION: Seventy-five mg of streptozotocin per kg BW can induce a diabetic nephropathy animal model in Wistar norvegicus rats. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12902-023-01504-1. BioMed Central 2023-11-22 /pmc/articles/PMC10664383/ /pubmed/37990213 http://dx.doi.org/10.1186/s12902-023-01504-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wahono, Aditya Mahardika
Harnanik, Titut
Pasaribu, Irma A.
Adiwinoto, Ronald Pratama
Octavianda, Yohana
Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin
title Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin
title_full Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin
title_fullStr Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin
title_full_unstemmed Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin
title_short Laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin
title_sort laboratory and clinical findings in mouse models of diabetic nephropathy induced with streptozotocin
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10664383/
https://www.ncbi.nlm.nih.gov/pubmed/37990213
http://dx.doi.org/10.1186/s12902-023-01504-1
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