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Unravelling the genome of the brackish water malaria vector Anopheles aquasalis
Malaria is a severe public health problem in several developing tropical and subtropical countries. Anopheles aquasalis is the primary coastal malaria vector in Central and South America and the Caribbean Islands, and it has the peculiar feature of living in water with large changes in salinity. Rec...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665375/ https://www.ncbi.nlm.nih.gov/pubmed/37993652 http://dx.doi.org/10.1038/s41598-023-47830-1 |
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author | Alencar, Rodrigo Maciel Sepulveda, Cesar Camilo Prado Martinez-Villegas, Luis Bahia, Ana Cristina Santana, Rosa Amélia de Souza, Igor Belém D’Elia, Gigliola Mayara Ayres Duarte, Ana Paula Marques de Lacerda, Marcus Vinicius Guimarães Monteiro, Wuelton Marcelo Secundino, Nágila Francinete Costa Pimenta, Paulo Filemon Paolucci Koerich, Leonardo Barbosa |
author_facet | Alencar, Rodrigo Maciel Sepulveda, Cesar Camilo Prado Martinez-Villegas, Luis Bahia, Ana Cristina Santana, Rosa Amélia de Souza, Igor Belém D’Elia, Gigliola Mayara Ayres Duarte, Ana Paula Marques de Lacerda, Marcus Vinicius Guimarães Monteiro, Wuelton Marcelo Secundino, Nágila Francinete Costa Pimenta, Paulo Filemon Paolucci Koerich, Leonardo Barbosa |
author_sort | Alencar, Rodrigo Maciel |
collection | PubMed |
description | Malaria is a severe public health problem in several developing tropical and subtropical countries. Anopheles aquasalis is the primary coastal malaria vector in Central and South America and the Caribbean Islands, and it has the peculiar feature of living in water with large changes in salinity. Recent research has recognised An. aquasalis as an important model for studying the interactions of murine and human Plasmodium parasites. This study presents the complete genome of An. aquasalis and offers insights into its evolution and physiology. The genome is similar in size and gene content to other Neotropical anophelines, with 162 Mb and 12,446 protein-coding genes. There are 1387 single-copy orthologs at the Diptera level (eg. An. gambiae, An. darlingi and Drosophila melanogaster). An. aquasalis diverged from An. darlingi, the primary malaria vector in inland South America, nearly 20 million years ago. Proteins related to ion transport and metabolism belong to the most abundant gene families with 660 genes. We identified gene families relevant to osmosis control (e.g., aquaporins, vacuolar-ATPases, Na+/K+-ATPases, and carbonic anhydrases). Evolutionary analysis suggests that all osmotic regulation genes are under strong purifying selection. We also observed low copy number variation in insecticide resistance and immunity-related genes for all known classical pathways. The data provided by this study offers candidate genes for further studies of parasite-vector interactions and for studies on how anophelines of brackish water deal with the high fluctuation in water salinity. We also established data and insights supporting An. aquasalis as an emerging Neotropical malaria vector model for genetic and molecular studies. |
format | Online Article Text |
id | pubmed-10665375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106653752023-11-22 Unravelling the genome of the brackish water malaria vector Anopheles aquasalis Alencar, Rodrigo Maciel Sepulveda, Cesar Camilo Prado Martinez-Villegas, Luis Bahia, Ana Cristina Santana, Rosa Amélia de Souza, Igor Belém D’Elia, Gigliola Mayara Ayres Duarte, Ana Paula Marques de Lacerda, Marcus Vinicius Guimarães Monteiro, Wuelton Marcelo Secundino, Nágila Francinete Costa Pimenta, Paulo Filemon Paolucci Koerich, Leonardo Barbosa Sci Rep Article Malaria is a severe public health problem in several developing tropical and subtropical countries. Anopheles aquasalis is the primary coastal malaria vector in Central and South America and the Caribbean Islands, and it has the peculiar feature of living in water with large changes in salinity. Recent research has recognised An. aquasalis as an important model for studying the interactions of murine and human Plasmodium parasites. This study presents the complete genome of An. aquasalis and offers insights into its evolution and physiology. The genome is similar in size and gene content to other Neotropical anophelines, with 162 Mb and 12,446 protein-coding genes. There are 1387 single-copy orthologs at the Diptera level (eg. An. gambiae, An. darlingi and Drosophila melanogaster). An. aquasalis diverged from An. darlingi, the primary malaria vector in inland South America, nearly 20 million years ago. Proteins related to ion transport and metabolism belong to the most abundant gene families with 660 genes. We identified gene families relevant to osmosis control (e.g., aquaporins, vacuolar-ATPases, Na+/K+-ATPases, and carbonic anhydrases). Evolutionary analysis suggests that all osmotic regulation genes are under strong purifying selection. We also observed low copy number variation in insecticide resistance and immunity-related genes for all known classical pathways. The data provided by this study offers candidate genes for further studies of parasite-vector interactions and for studies on how anophelines of brackish water deal with the high fluctuation in water salinity. We also established data and insights supporting An. aquasalis as an emerging Neotropical malaria vector model for genetic and molecular studies. Nature Publishing Group UK 2023-11-22 /pmc/articles/PMC10665375/ /pubmed/37993652 http://dx.doi.org/10.1038/s41598-023-47830-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Alencar, Rodrigo Maciel Sepulveda, Cesar Camilo Prado Martinez-Villegas, Luis Bahia, Ana Cristina Santana, Rosa Amélia de Souza, Igor Belém D’Elia, Gigliola Mayara Ayres Duarte, Ana Paula Marques de Lacerda, Marcus Vinicius Guimarães Monteiro, Wuelton Marcelo Secundino, Nágila Francinete Costa Pimenta, Paulo Filemon Paolucci Koerich, Leonardo Barbosa Unravelling the genome of the brackish water malaria vector Anopheles aquasalis |
title | Unravelling the genome of the brackish water malaria vector Anopheles aquasalis |
title_full | Unravelling the genome of the brackish water malaria vector Anopheles aquasalis |
title_fullStr | Unravelling the genome of the brackish water malaria vector Anopheles aquasalis |
title_full_unstemmed | Unravelling the genome of the brackish water malaria vector Anopheles aquasalis |
title_short | Unravelling the genome of the brackish water malaria vector Anopheles aquasalis |
title_sort | unravelling the genome of the brackish water malaria vector anopheles aquasalis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665375/ https://www.ncbi.nlm.nih.gov/pubmed/37993652 http://dx.doi.org/10.1038/s41598-023-47830-1 |
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