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Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar

Heartworm disease in dogs and cats caused by Dirofilaria immitis continues to be a major clinical issue globally. This study focused on dogs suspicious of having tick-borne diseases (TBD) brought to a clinic and a veterinary teaching hospital in Myanmar. Blood samples were collected and initially sc...

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Autores principales: Bawm, Saw, Khaing, Yadanar, Chel, Hla Myet, Hmoon, Myint Myint, Win, Shwe Yee, Bo, Min, Naing, Tint, Htun, Lat Lat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665652/
https://www.ncbi.nlm.nih.gov/pubmed/38021190
http://dx.doi.org/10.1016/j.crpvbd.2023.100148
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author Bawm, Saw
Khaing, Yadanar
Chel, Hla Myet
Hmoon, Myint Myint
Win, Shwe Yee
Bo, Min
Naing, Tint
Htun, Lat Lat
author_facet Bawm, Saw
Khaing, Yadanar
Chel, Hla Myet
Hmoon, Myint Myint
Win, Shwe Yee
Bo, Min
Naing, Tint
Htun, Lat Lat
author_sort Bawm, Saw
collection PubMed
description Heartworm disease in dogs and cats caused by Dirofilaria immitis continues to be a major clinical issue globally. This study focused on dogs suspicious of having tick-borne diseases (TBD) brought to a clinic and a veterinary teaching hospital in Myanmar. Blood samples were collected and initially screened using SNAP® 4Dx® Plus test kit. All dog blood samples were subjected to conventional PCR to detect both Dirofilaria spp. (cox1 gene) and Wolbachia spp. (16S rDNA) infections. Infection with D. immitis was detected in 14 (28.0%) of 50 examined samples, while the detection rate of TBD causative agents, including Anaplasma phagocytophilum and Ehrlichia canis, was 26.0% (13/50) and 26.0% (13/50), respectively, as determined by ELISA rapid test. In this study, D. immitis infection was moderately but significantly correlated with TBD infections (Pearson’s r = 0.397, P = 0.008). Comparative sequence and phylogenetic analyses provided molecular identification of D. immitis in Myanmar and confirmed the identity of its Wolbachia endosymbiont with Wolbachia endosymbionts isolated from D. immitis, Rhipicephalus sanguineus and Aedes aegypti. The present study contributes to our understanding of the coexistence of D. immitis and Wolbachia endosymbiosis in dogs, and the findings may benefit the future prevention and control of dirofilariasis in dogs.
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spelling pubmed-106656522023-10-04 Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar Bawm, Saw Khaing, Yadanar Chel, Hla Myet Hmoon, Myint Myint Win, Shwe Yee Bo, Min Naing, Tint Htun, Lat Lat Curr Res Parasitol Vector Borne Dis Research Article Heartworm disease in dogs and cats caused by Dirofilaria immitis continues to be a major clinical issue globally. This study focused on dogs suspicious of having tick-borne diseases (TBD) brought to a clinic and a veterinary teaching hospital in Myanmar. Blood samples were collected and initially screened using SNAP® 4Dx® Plus test kit. All dog blood samples were subjected to conventional PCR to detect both Dirofilaria spp. (cox1 gene) and Wolbachia spp. (16S rDNA) infections. Infection with D. immitis was detected in 14 (28.0%) of 50 examined samples, while the detection rate of TBD causative agents, including Anaplasma phagocytophilum and Ehrlichia canis, was 26.0% (13/50) and 26.0% (13/50), respectively, as determined by ELISA rapid test. In this study, D. immitis infection was moderately but significantly correlated with TBD infections (Pearson’s r = 0.397, P = 0.008). Comparative sequence and phylogenetic analyses provided molecular identification of D. immitis in Myanmar and confirmed the identity of its Wolbachia endosymbiont with Wolbachia endosymbionts isolated from D. immitis, Rhipicephalus sanguineus and Aedes aegypti. The present study contributes to our understanding of the coexistence of D. immitis and Wolbachia endosymbiosis in dogs, and the findings may benefit the future prevention and control of dirofilariasis in dogs. Elsevier 2023-10-04 /pmc/articles/PMC10665652/ /pubmed/38021190 http://dx.doi.org/10.1016/j.crpvbd.2023.100148 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Bawm, Saw
Khaing, Yadanar
Chel, Hla Myet
Hmoon, Myint Myint
Win, Shwe Yee
Bo, Min
Naing, Tint
Htun, Lat Lat
Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar
title Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar
title_full Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar
title_fullStr Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar
title_full_unstemmed Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar
title_short Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar
title_sort molecular detection of dirofilaria immitis and its wolbachia endosymbionts in dogs from myanmar
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665652/
https://www.ncbi.nlm.nih.gov/pubmed/38021190
http://dx.doi.org/10.1016/j.crpvbd.2023.100148
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