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A novel long-tailed myovirus represents a new T4-like cyanophage cluster

Cyanophages affect the abundance, diversity, metabolism, and evolution of picocyanobacteria in marine ecosystems. Here we report an estuarine Synechococcus phage, S-CREM2, which represents a novel viral genus and leads to the establishment of a new T4-like cyanophage clade named cluster C. S-CREM2 p...

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Autores principales: Liu, Yuanfang, Meng, Xue, Zheng, Hongrui, Cai, Lanlan, Wei, Shuzhen, He, Minglu, He, Jiale, Hao, Yue, Ge, Chang, Liu, Jihua, Chen, Feng, Xu, Yongle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665884/
https://www.ncbi.nlm.nih.gov/pubmed/38029084
http://dx.doi.org/10.3389/fmicb.2023.1293846
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author Liu, Yuanfang
Meng, Xue
Zheng, Hongrui
Cai, Lanlan
Wei, Shuzhen
He, Minglu
He, Jiale
Hao, Yue
Ge, Chang
Liu, Jihua
Chen, Feng
Xu, Yongle
author_facet Liu, Yuanfang
Meng, Xue
Zheng, Hongrui
Cai, Lanlan
Wei, Shuzhen
He, Minglu
He, Jiale
Hao, Yue
Ge, Chang
Liu, Jihua
Chen, Feng
Xu, Yongle
author_sort Liu, Yuanfang
collection PubMed
description Cyanophages affect the abundance, diversity, metabolism, and evolution of picocyanobacteria in marine ecosystems. Here we report an estuarine Synechococcus phage, S-CREM2, which represents a novel viral genus and leads to the establishment of a new T4-like cyanophage clade named cluster C. S-CREM2 possesses the longest tail (~418 nm) among isolated cyanomyoviruses and encodes six tail-related proteins that are exclusively homologous to those predicted in the cluster C cyanophages. Furthermore, S-CREM2 may carry three regulatory proteins in the virion, which may play a crucial role in optimizing the host intracellular environment for viral replication at the initial stage of infection. The cluster C cyanophages lack auxiliary metabolic genes (AMGs) that are commonly found in cyanophages of the T4-like clusters A and B and encode unique AMGs like an S-type phycobilin lyase gene. A variation in the composition of tRNA and cis-regulatory RNA genes was observed between the marine and freshwater phage strains in cluster C, reflecting their different modes of coping with hosts and habitats. The cluster C cyanophages are widespread in estuarine and coastal regions and exhibit equivalent or even higher relative abundance compared to those of clusters A and B cyanophages in certain estuarine regions. The isolation of cyanophage S-CREM2 provides new insights into the phage–host interactions mediated by both newly discovered AMGs and virion-associated proteins and emphasizes the ecological significance of cluster C cyanophages in estuarine environments.
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spelling pubmed-106658842023-11-09 A novel long-tailed myovirus represents a new T4-like cyanophage cluster Liu, Yuanfang Meng, Xue Zheng, Hongrui Cai, Lanlan Wei, Shuzhen He, Minglu He, Jiale Hao, Yue Ge, Chang Liu, Jihua Chen, Feng Xu, Yongle Front Microbiol Microbiology Cyanophages affect the abundance, diversity, metabolism, and evolution of picocyanobacteria in marine ecosystems. Here we report an estuarine Synechococcus phage, S-CREM2, which represents a novel viral genus and leads to the establishment of a new T4-like cyanophage clade named cluster C. S-CREM2 possesses the longest tail (~418 nm) among isolated cyanomyoviruses and encodes six tail-related proteins that are exclusively homologous to those predicted in the cluster C cyanophages. Furthermore, S-CREM2 may carry three regulatory proteins in the virion, which may play a crucial role in optimizing the host intracellular environment for viral replication at the initial stage of infection. The cluster C cyanophages lack auxiliary metabolic genes (AMGs) that are commonly found in cyanophages of the T4-like clusters A and B and encode unique AMGs like an S-type phycobilin lyase gene. A variation in the composition of tRNA and cis-regulatory RNA genes was observed between the marine and freshwater phage strains in cluster C, reflecting their different modes of coping with hosts and habitats. The cluster C cyanophages are widespread in estuarine and coastal regions and exhibit equivalent or even higher relative abundance compared to those of clusters A and B cyanophages in certain estuarine regions. The isolation of cyanophage S-CREM2 provides new insights into the phage–host interactions mediated by both newly discovered AMGs and virion-associated proteins and emphasizes the ecological significance of cluster C cyanophages in estuarine environments. Frontiers Media S.A. 2023-11-09 /pmc/articles/PMC10665884/ /pubmed/38029084 http://dx.doi.org/10.3389/fmicb.2023.1293846 Text en Copyright © 2023 Liu, Meng, Zheng, Cai, Wei, He, He, Hao, Ge, Liu, Chen and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Yuanfang
Meng, Xue
Zheng, Hongrui
Cai, Lanlan
Wei, Shuzhen
He, Minglu
He, Jiale
Hao, Yue
Ge, Chang
Liu, Jihua
Chen, Feng
Xu, Yongle
A novel long-tailed myovirus represents a new T4-like cyanophage cluster
title A novel long-tailed myovirus represents a new T4-like cyanophage cluster
title_full A novel long-tailed myovirus represents a new T4-like cyanophage cluster
title_fullStr A novel long-tailed myovirus represents a new T4-like cyanophage cluster
title_full_unstemmed A novel long-tailed myovirus represents a new T4-like cyanophage cluster
title_short A novel long-tailed myovirus represents a new T4-like cyanophage cluster
title_sort novel long-tailed myovirus represents a new t4-like cyanophage cluster
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665884/
https://www.ncbi.nlm.nih.gov/pubmed/38029084
http://dx.doi.org/10.3389/fmicb.2023.1293846
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