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Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios

In dynamic microbial ecosystems, bacterial communication is a relevant mechanism for interactions between different microbial species. When C. jejuni resides in the intestine of either avian or human hosts, it is exposed to diverse bacteria from the microbiome. This study aimed to reveal the influen...

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Autores principales: Dreyer, Annika, Lenz, Christof, Groß, Uwe, Bohne, Wolfgang, Zautner, Andreas Erich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10666060/
https://www.ncbi.nlm.nih.gov/pubmed/38029072
http://dx.doi.org/10.3389/fmicb.2023.1247211
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author Dreyer, Annika
Lenz, Christof
Groß, Uwe
Bohne, Wolfgang
Zautner, Andreas Erich
author_facet Dreyer, Annika
Lenz, Christof
Groß, Uwe
Bohne, Wolfgang
Zautner, Andreas Erich
author_sort Dreyer, Annika
collection PubMed
description In dynamic microbial ecosystems, bacterial communication is a relevant mechanism for interactions between different microbial species. When C. jejuni resides in the intestine of either avian or human hosts, it is exposed to diverse bacteria from the microbiome. This study aimed to reveal the influence of co-incubation with Enterococcus faecalis, Enterococcus faecium, or Staphylococcus aureus on the proteome of C. jejuni 81–176 using data-independent-acquisition mass spectrometry (DIA-MS). We compared the proteome profiles during co-incubation with the proteome profile in response to the bile acid deoxycholate (DCA) and investigated the impact of DCA on proteomic changes during co-incubation, as C. jejuni is exposed to both factors during colonization. We identified 1,375 proteins by DIA-MS, which is notably high, approaching the theoretical maximum of 1,645 proteins. S. aureus had the highest impact on the proteome of C. jejuni with 215 up-regulated and 230 down-regulated proteins. However, these numbers are still markedly lower than the 526 up-regulated and 516 down-regulated proteins during DCA exposure. We identified a subset of 54 significantly differentially expressed proteins that are shared after co-incubation with all three microbial species. These proteins were indicative of a common co-incubation response of C. jejuni. This common proteomic response partly overlapped with the DCA response; however, several proteins were specific to the co-incubation response. In the co-incubation experiment, we identified three membrane-interactive proteins among the top 20 up-regulated proteins. This finding suggests that the presence of other bacteria may contribute to increased adherence, e.g., to other bacteria but eventually also epithelial cells or abiotic surfaces. Furthermore, a conjugative transfer regulon protein was typically up-expressed during co-incubation. Exposure to both, co-incubation and DCA, demonstrated that the two stressors influenced each other, resulting in a unique synergistic proteomic response that differed from the response to each stimulus alone. Data are available via ProteomeXchange with identifier PXD046477.
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spelling pubmed-106660602023-11-09 Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios Dreyer, Annika Lenz, Christof Groß, Uwe Bohne, Wolfgang Zautner, Andreas Erich Front Microbiol Microbiology In dynamic microbial ecosystems, bacterial communication is a relevant mechanism for interactions between different microbial species. When C. jejuni resides in the intestine of either avian or human hosts, it is exposed to diverse bacteria from the microbiome. This study aimed to reveal the influence of co-incubation with Enterococcus faecalis, Enterococcus faecium, or Staphylococcus aureus on the proteome of C. jejuni 81–176 using data-independent-acquisition mass spectrometry (DIA-MS). We compared the proteome profiles during co-incubation with the proteome profile in response to the bile acid deoxycholate (DCA) and investigated the impact of DCA on proteomic changes during co-incubation, as C. jejuni is exposed to both factors during colonization. We identified 1,375 proteins by DIA-MS, which is notably high, approaching the theoretical maximum of 1,645 proteins. S. aureus had the highest impact on the proteome of C. jejuni with 215 up-regulated and 230 down-regulated proteins. However, these numbers are still markedly lower than the 526 up-regulated and 516 down-regulated proteins during DCA exposure. We identified a subset of 54 significantly differentially expressed proteins that are shared after co-incubation with all three microbial species. These proteins were indicative of a common co-incubation response of C. jejuni. This common proteomic response partly overlapped with the DCA response; however, several proteins were specific to the co-incubation response. In the co-incubation experiment, we identified three membrane-interactive proteins among the top 20 up-regulated proteins. This finding suggests that the presence of other bacteria may contribute to increased adherence, e.g., to other bacteria but eventually also epithelial cells or abiotic surfaces. Furthermore, a conjugative transfer regulon protein was typically up-expressed during co-incubation. Exposure to both, co-incubation and DCA, demonstrated that the two stressors influenced each other, resulting in a unique synergistic proteomic response that differed from the response to each stimulus alone. Data are available via ProteomeXchange with identifier PXD046477. Frontiers Media S.A. 2023-11-09 /pmc/articles/PMC10666060/ /pubmed/38029072 http://dx.doi.org/10.3389/fmicb.2023.1247211 Text en Copyright © 2023 Dreyer, Lenz, Groß, Bohne and Zautner. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dreyer, Annika
Lenz, Christof
Groß, Uwe
Bohne, Wolfgang
Zautner, Andreas Erich
Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
title Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
title_full Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
title_fullStr Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
title_full_unstemmed Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
title_short Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
title_sort characterization of campylobacter jejuni proteome profiles in co-incubation scenarios
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10666060/
https://www.ncbi.nlm.nih.gov/pubmed/38029072
http://dx.doi.org/10.3389/fmicb.2023.1247211
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