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A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol

BACKGROUND: In the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is hampered in plants due to lack of robust validation methods. Likewise, modeling of plant development is hampered by paucity of spatial gene expression...

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Detalles Bibliográficos
Autores principales:	Huang, Tian, Guillotin, Bruno, Rahni, Ramin, Birnbaum, Kenneth D., Wagner, Doris
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10666358/ https://www.ncbi.nlm.nih.gov/pubmed/37993896 http://dx.doi.org/10.1186/s13007-023-01108-9

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