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Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome
Objective: The aim of this study was to analyze and compare the differential expression of peptides within the follicular fluid of polycystic ovary syndrome (PCOS) patients versus normal women by using peptidomics techniques. The underlying mechanisms involved in PCOS pathogenesis will be explored,...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10666747/ https://www.ncbi.nlm.nih.gov/pubmed/38020909 http://dx.doi.org/10.3389/fcell.2023.1289063 |
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author | Sun, Ningyu Chen, Yuanyuan Lu, Lu Yan, Hua Zhou, Jing Li, Kai Zhang, Wuwen Yuan, Lihua Heng, Boon Chin Zeng, Weiwei Shi, Yin Tong, Guoqing Yin, Ping |
author_facet | Sun, Ningyu Chen, Yuanyuan Lu, Lu Yan, Hua Zhou, Jing Li, Kai Zhang, Wuwen Yuan, Lihua Heng, Boon Chin Zeng, Weiwei Shi, Yin Tong, Guoqing Yin, Ping |
author_sort | Sun, Ningyu |
collection | PubMed |
description | Objective: The aim of this study was to analyze and compare the differential expression of peptides within the follicular fluid of polycystic ovary syndrome (PCOS) patients versus normal women by using peptidomics techniques. The underlying mechanisms involved in PCOS pathogenesis will be explored, together with screening and identification of potential functional peptides via bioinformatics analysis. Materials and methods: A total of 12 patients who underwent in vitro fertilization and embryo transfer (IVF-ET) at the Reproductive Medicine Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from 1 September 2022 to 1 November 2022 were included in this study. The follicular fluid of PCOS patients (n = 6) and normal women (n = 6) were collected. The presence and concentration differences of various peptides were detected by the LC-MS/MS method. GO and KEGG analysis were performed on the precursor proteins of the differentially-expressed peptides, and protein network interaction analysis was carried out to identify functionally-relevant peptides among the various peptides. Results: A variety of peptides within the follicular fluid of PCOS versus normal patients were detected by peptidomics techniques. Altogether, 843 upregulated peptides and 236 downregulated peptides were detected (absolute fold change ≥2 and p < 0.05). Of these, 718 (718 = 488 + 230) peptides were only detected in the PCOS group, while 205 (205 = 174 + 31) were only detected in the control group. Gene Ontology enrichment and pathway analysis were performed to characterize peptides through their precursor proteins. We identified 18 peptides from 7 precursor proteins associated with PCOS, and 4 peptide sequences were located in the functional domains of their corresponding precursor proteins. Conclusion: In this study, differences in the follicular development of PCOS versus normal patients were revealed from the polypeptidomics of follicular development, which thus provided new insights for future studies on the pathological mechanisms of PCOS development. |
format | Online Article Text |
id | pubmed-10666747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106667472023-01-01 Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome Sun, Ningyu Chen, Yuanyuan Lu, Lu Yan, Hua Zhou, Jing Li, Kai Zhang, Wuwen Yuan, Lihua Heng, Boon Chin Zeng, Weiwei Shi, Yin Tong, Guoqing Yin, Ping Front Cell Dev Biol Cell and Developmental Biology Objective: The aim of this study was to analyze and compare the differential expression of peptides within the follicular fluid of polycystic ovary syndrome (PCOS) patients versus normal women by using peptidomics techniques. The underlying mechanisms involved in PCOS pathogenesis will be explored, together with screening and identification of potential functional peptides via bioinformatics analysis. Materials and methods: A total of 12 patients who underwent in vitro fertilization and embryo transfer (IVF-ET) at the Reproductive Medicine Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from 1 September 2022 to 1 November 2022 were included in this study. The follicular fluid of PCOS patients (n = 6) and normal women (n = 6) were collected. The presence and concentration differences of various peptides were detected by the LC-MS/MS method. GO and KEGG analysis were performed on the precursor proteins of the differentially-expressed peptides, and protein network interaction analysis was carried out to identify functionally-relevant peptides among the various peptides. Results: A variety of peptides within the follicular fluid of PCOS versus normal patients were detected by peptidomics techniques. Altogether, 843 upregulated peptides and 236 downregulated peptides were detected (absolute fold change ≥2 and p < 0.05). Of these, 718 (718 = 488 + 230) peptides were only detected in the PCOS group, while 205 (205 = 174 + 31) were only detected in the control group. Gene Ontology enrichment and pathway analysis were performed to characterize peptides through their precursor proteins. We identified 18 peptides from 7 precursor proteins associated with PCOS, and 4 peptide sequences were located in the functional domains of their corresponding precursor proteins. Conclusion: In this study, differences in the follicular development of PCOS versus normal patients were revealed from the polypeptidomics of follicular development, which thus provided new insights for future studies on the pathological mechanisms of PCOS development. Frontiers Media S.A. 2023-11-08 /pmc/articles/PMC10666747/ /pubmed/38020909 http://dx.doi.org/10.3389/fcell.2023.1289063 Text en Copyright © 2023 Sun, Chen, Lu, Yan, Zhou, Li, Zhang, Yuan, Heng, Zeng, Shi, Tong and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Sun, Ningyu Chen, Yuanyuan Lu, Lu Yan, Hua Zhou, Jing Li, Kai Zhang, Wuwen Yuan, Lihua Heng, Boon Chin Zeng, Weiwei Shi, Yin Tong, Guoqing Yin, Ping Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome |
title | Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome |
title_full | Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome |
title_fullStr | Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome |
title_full_unstemmed | Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome |
title_short | Peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome |
title_sort | peptidomic analysis of follicular fluid in patients with polycystic ovarian syndrome |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10666747/ https://www.ncbi.nlm.nih.gov/pubmed/38020909 http://dx.doi.org/10.3389/fcell.2023.1289063 |
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