Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics

We describe construction of the synthetic yeast chromosome XI (synXI) and reveal the effects of redesign at non-coding DNA elements. The 660-kb synthetic yeast genome project (Sc2.0) chromosome was assembled from synthesized DNA fragments before CRISPR-based methods were used in a process of bug dis...

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Detalles Bibliográficos
Autores principales: Blount, Benjamin A., Lu, Xinyu, Driessen, Maureen R.M., Jovicevic, Dejana, Sanchez, Mateo I., Ciurkot, Klaudia, Zhao, Yu, Lauer, Stephanie, McKiernan, Robert M., Gowers, Glen-Oliver F., Sweeney, Fiachra, Fanfani, Viola, Lobzaev, Evgenii, Palacios-Flores, Kim, Walker, Roy S.K., Hesketh, Andy, Cai, Jitong, Oliver, Stephen G., Cai, Yizhi, Stracquadanio, Giovanni, Mitchell, Leslie A., Bader, Joel S., Boeke, Jef D., Ellis, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667340/
https://www.ncbi.nlm.nih.gov/pubmed/38020971
http://dx.doi.org/10.1016/j.xgen.2023.100418

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