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The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix ZSY‐33
Resolving the heavy metal resistance mechanisms of microbes is crucial for understanding the bioremediation of the ecological environment. In this study, a multiple heavy metal resistance bacterium, Pseudoxanthomonas spadix ZSY‐33 was isolated and characterized. The copper resistance mechanism was r...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667631/ https://www.ncbi.nlm.nih.gov/pubmed/37328952 http://dx.doi.org/10.1111/1758-2229.13163 |
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author | Wang, Hongjie Zhang, Siyao Zhang, Jing |
author_facet | Wang, Hongjie Zhang, Siyao Zhang, Jing |
author_sort | Wang, Hongjie |
collection | PubMed |
description | Resolving the heavy metal resistance mechanisms of microbes is crucial for understanding the bioremediation of the ecological environment. In this study, a multiple heavy metal resistance bacterium, Pseudoxanthomonas spadix ZSY‐33 was isolated and characterized. The copper resistance mechanism was revealed by analysis of the physiological traits, copper distribution, and genomic and transcriptomic data of strain ZSY‐33 cultured with different concentrations of copper. The growth inhibition assay in basic medium showed that the growth of strain ZSY‐33 was inhibited in the presence of 0.5 mM copper. The production of extracellular polymeric substances increased at a lower concentration of copper and decreased at a higher concentration of copper. Integrative analysis of genomic and transcriptomic, the copper resistance mechanism in strain ZSY‐33 was elucidated. At a lower concentration of copper, the Cus and Cop systems were responsible for the homeostasis of intracellular copper. As the concentration of copper increased, multiple metabolism pathways, including the metabolism of sulfur, amino acids, and pro‐energy were cooperated with the Cus and Cop systems to deal with copper stress. These results indicated a flexible copper resistance mechanism in strain ZSY‐33, which may acquire from the long‐term interaction with the living environment. |
format | Online Article Text |
id | pubmed-10667631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106676312023-06-16 The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix ZSY‐33 Wang, Hongjie Zhang, Siyao Zhang, Jing Environ Microbiol Rep Research Articles Resolving the heavy metal resistance mechanisms of microbes is crucial for understanding the bioremediation of the ecological environment. In this study, a multiple heavy metal resistance bacterium, Pseudoxanthomonas spadix ZSY‐33 was isolated and characterized. The copper resistance mechanism was revealed by analysis of the physiological traits, copper distribution, and genomic and transcriptomic data of strain ZSY‐33 cultured with different concentrations of copper. The growth inhibition assay in basic medium showed that the growth of strain ZSY‐33 was inhibited in the presence of 0.5 mM copper. The production of extracellular polymeric substances increased at a lower concentration of copper and decreased at a higher concentration of copper. Integrative analysis of genomic and transcriptomic, the copper resistance mechanism in strain ZSY‐33 was elucidated. At a lower concentration of copper, the Cus and Cop systems were responsible for the homeostasis of intracellular copper. As the concentration of copper increased, multiple metabolism pathways, including the metabolism of sulfur, amino acids, and pro‐energy were cooperated with the Cus and Cop systems to deal with copper stress. These results indicated a flexible copper resistance mechanism in strain ZSY‐33, which may acquire from the long‐term interaction with the living environment. John Wiley & Sons, Inc. 2023-06-16 /pmc/articles/PMC10667631/ /pubmed/37328952 http://dx.doi.org/10.1111/1758-2229.13163 Text en © 2023 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Wang, Hongjie Zhang, Siyao Zhang, Jing The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix ZSY‐33 |
title | The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix
ZSY‐33 |
title_full | The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix
ZSY‐33 |
title_fullStr | The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix
ZSY‐33 |
title_full_unstemmed | The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix
ZSY‐33 |
title_short | The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadix
ZSY‐33 |
title_sort | copper resistance mechanism in a newly isolated pseudoxanthomonas spadix
zsy‐33 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667631/ https://www.ncbi.nlm.nih.gov/pubmed/37328952 http://dx.doi.org/10.1111/1758-2229.13163 |
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