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Screening of potential donors for anaerobic stress tolerance during germination in rice

The rising cost of transplanting rice has made direct seeding an affordable alternative for rice establishment, particularly in Africa. However, direct seeding, while cost-effective, faces crop establishment challenges due to flooding. Uncontrolled water, driven by erratic rains in low-lying areas o...

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Autores principales: Mwakyusa, Lupakisyo, Heredia, Maria Cristina, Kilasi, Newton Lwiyiso, Madege, Richard R., Herzog, Max, Dixit, Shalabh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667690/
https://www.ncbi.nlm.nih.gov/pubmed/38023850
http://dx.doi.org/10.3389/fpls.2023.1261101
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author Mwakyusa, Lupakisyo
Heredia, Maria Cristina
Kilasi, Newton Lwiyiso
Madege, Richard R.
Herzog, Max
Dixit, Shalabh
author_facet Mwakyusa, Lupakisyo
Heredia, Maria Cristina
Kilasi, Newton Lwiyiso
Madege, Richard R.
Herzog, Max
Dixit, Shalabh
author_sort Mwakyusa, Lupakisyo
collection PubMed
description The rising cost of transplanting rice has made direct seeding an affordable alternative for rice establishment, particularly in Africa. However, direct seeding, while cost-effective, faces crop establishment challenges due to flooding. Uncontrolled water, driven by erratic rains in low-lying areas or uneven fields, limit germination. Rice possesses the unique ability of anaerobic germination, enabling it to sprout and emerge in oxygen-deprived conditions. Understanding rice’s response to anaerobic stress during germination is crucial for resilience breeding. Africa, although relying on direct seeding, has made limited progress in addressing flooding during germination compared to Asia. Anaerobic stress tolerance ensures successful crop emergence even in oxygen-limited environments and can help suppress weeds, a significant challenge in direct-seeded rice cultivation. This study aims to contribute by screening for potential rice donors exhibiting anaerobic stress tolerance. We screened 200 rice genotypes at Sokoine University of Agriculture (SUA) in Morogoro, Tanzania, primarily focusing on landraces with untapped potential. Using an alpha lattice design, we conducted two anaerobic experiments in September and October 2022, adding 7 cm of standing water immediately after dry seeding for flooded and maintaining a 2 cm water level after germination in the control for duration of 21 days. We identified potential donors based on selection index computed from genomic estimated breeding values (GEBVs) using eight variables: germination at 14 DAS, germination at 21 DAS, seedling height at 14 DAS, seedling height at 21 DAS, shoot dry matter at 21 DAS, root dry matter at 21 DAS, culm diameter at 21 DAS, and root length at 21DAS. Ten genotypes emerged as the most promising, exhibiting at least 70% germination in floodwater at 21 DAS and greater selection indices. These genotypes were like: Afaa Mwanza 1/159, Rojomena 271/10, Kubwa Jinga, Wahiwahi, Magongo ya Wayungu, Mpaka wa Bibi, Mwangaza, Tarabinzona, IB126-Bug 2013A, and Kanamalia with respective percentages of 75, 74, 71, 86, 75, 80, 71, 80, 70, and 73. These findings contribute to global efforts to mitigate the impacts of flooding during germination. These donors, will be potential to enrich the gene pool for anaerobic germination, providing valuable resources for breeding for flooding tolerance.
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spelling pubmed-106676902023-01-01 Screening of potential donors for anaerobic stress tolerance during germination in rice Mwakyusa, Lupakisyo Heredia, Maria Cristina Kilasi, Newton Lwiyiso Madege, Richard R. Herzog, Max Dixit, Shalabh Front Plant Sci Plant Science The rising cost of transplanting rice has made direct seeding an affordable alternative for rice establishment, particularly in Africa. However, direct seeding, while cost-effective, faces crop establishment challenges due to flooding. Uncontrolled water, driven by erratic rains in low-lying areas or uneven fields, limit germination. Rice possesses the unique ability of anaerobic germination, enabling it to sprout and emerge in oxygen-deprived conditions. Understanding rice’s response to anaerobic stress during germination is crucial for resilience breeding. Africa, although relying on direct seeding, has made limited progress in addressing flooding during germination compared to Asia. Anaerobic stress tolerance ensures successful crop emergence even in oxygen-limited environments and can help suppress weeds, a significant challenge in direct-seeded rice cultivation. This study aims to contribute by screening for potential rice donors exhibiting anaerobic stress tolerance. We screened 200 rice genotypes at Sokoine University of Agriculture (SUA) in Morogoro, Tanzania, primarily focusing on landraces with untapped potential. Using an alpha lattice design, we conducted two anaerobic experiments in September and October 2022, adding 7 cm of standing water immediately after dry seeding for flooded and maintaining a 2 cm water level after germination in the control for duration of 21 days. We identified potential donors based on selection index computed from genomic estimated breeding values (GEBVs) using eight variables: germination at 14 DAS, germination at 21 DAS, seedling height at 14 DAS, seedling height at 21 DAS, shoot dry matter at 21 DAS, root dry matter at 21 DAS, culm diameter at 21 DAS, and root length at 21DAS. Ten genotypes emerged as the most promising, exhibiting at least 70% germination in floodwater at 21 DAS and greater selection indices. These genotypes were like: Afaa Mwanza 1/159, Rojomena 271/10, Kubwa Jinga, Wahiwahi, Magongo ya Wayungu, Mpaka wa Bibi, Mwangaza, Tarabinzona, IB126-Bug 2013A, and Kanamalia with respective percentages of 75, 74, 71, 86, 75, 80, 71, 80, 70, and 73. These findings contribute to global efforts to mitigate the impacts of flooding during germination. These donors, will be potential to enrich the gene pool for anaerobic germination, providing valuable resources for breeding for flooding tolerance. Frontiers Media S.A. 2023-11-10 /pmc/articles/PMC10667690/ /pubmed/38023850 http://dx.doi.org/10.3389/fpls.2023.1261101 Text en Copyright © 2023 Mwakyusa, Heredia, Kilasi, Madege, Herzog and Dixit https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mwakyusa, Lupakisyo
Heredia, Maria Cristina
Kilasi, Newton Lwiyiso
Madege, Richard R.
Herzog, Max
Dixit, Shalabh
Screening of potential donors for anaerobic stress tolerance during germination in rice
title Screening of potential donors for anaerobic stress tolerance during germination in rice
title_full Screening of potential donors for anaerobic stress tolerance during germination in rice
title_fullStr Screening of potential donors for anaerobic stress tolerance during germination in rice
title_full_unstemmed Screening of potential donors for anaerobic stress tolerance during germination in rice
title_short Screening of potential donors for anaerobic stress tolerance during germination in rice
title_sort screening of potential donors for anaerobic stress tolerance during germination in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667690/
https://www.ncbi.nlm.nih.gov/pubmed/38023850
http://dx.doi.org/10.3389/fpls.2023.1261101
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