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Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i

As environmental opportunistic pathogens, nontuberculous mycobacteria (NTM) can cause severe and difficult to treat pulmonary disease. In the United States, Hawai’i has the highest prevalence of infection. Rapid growing mycobacteria (RGM) such as Mycobacterium abscessus and M. porcinum and the slow...

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Autores principales: Tan, Cara G., Oberlag, Nicole M., McGowan, Acelyn E., Dawrs, Stephanie N., Chan, Yvonne L., Strong, Michael, Hasan, Nabeeh A., Honda, Jennifer R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667711/
https://www.ncbi.nlm.nih.gov/pubmed/38029173
http://dx.doi.org/10.3389/fmicb.2023.1268963
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author Tan, Cara G.
Oberlag, Nicole M.
McGowan, Acelyn E.
Dawrs, Stephanie N.
Chan, Yvonne L.
Strong, Michael
Hasan, Nabeeh A.
Honda, Jennifer R.
author_facet Tan, Cara G.
Oberlag, Nicole M.
McGowan, Acelyn E.
Dawrs, Stephanie N.
Chan, Yvonne L.
Strong, Michael
Hasan, Nabeeh A.
Honda, Jennifer R.
author_sort Tan, Cara G.
collection PubMed
description As environmental opportunistic pathogens, nontuberculous mycobacteria (NTM) can cause severe and difficult to treat pulmonary disease. In the United States, Hawai’i has the highest prevalence of infection. Rapid growing mycobacteria (RGM) such as Mycobacterium abscessus and M. porcinum and the slow growing mycobacteria (SGM) including M. intracellulare subspecies chimaera are common environmental NTM species and subspecies in Hawai’i. Although iron acquisition is an essential process of many microorganisms, iron acquisition via siderophores among the NTM is not well-characterized. In this study, we apply genomic and microbiological methodologies to better understand iron acquisition via siderophores for environmental and respiratory isolates of M. abscessus, M. porcinum, and M. intracellulare subspecies chimaera from Hawai’i. Siderophore synthesis and transport genes, including mycobactin (mbt), mmpL/S, and esx-3 were compared among 47 reference isolates, 29 respiratory isolates, and 23 environmental Hawai’i isolates. Among all reference isolates examined, respiratory isolates showed significantly more siderophore pertinent genes compared to environmental isolates. Among the Hawai’i isolates, RGM M. abscessus and M. porcinum had significantly less esx-3 and mbt genes compared to SGM M. chimaera when stratified by growth classification. However, no significant differences were observed between the species when grown on low iron culture agar or siderophore production by the chrome azurol S (CAS) assay in vitro. These results indicate the complex mechanisms involved in iron sequestration and siderophore activity among diverse NTM species.
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spelling pubmed-106677112023-11-10 Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i Tan, Cara G. Oberlag, Nicole M. McGowan, Acelyn E. Dawrs, Stephanie N. Chan, Yvonne L. Strong, Michael Hasan, Nabeeh A. Honda, Jennifer R. Front Microbiol Microbiology As environmental opportunistic pathogens, nontuberculous mycobacteria (NTM) can cause severe and difficult to treat pulmonary disease. In the United States, Hawai’i has the highest prevalence of infection. Rapid growing mycobacteria (RGM) such as Mycobacterium abscessus and M. porcinum and the slow growing mycobacteria (SGM) including M. intracellulare subspecies chimaera are common environmental NTM species and subspecies in Hawai’i. Although iron acquisition is an essential process of many microorganisms, iron acquisition via siderophores among the NTM is not well-characterized. In this study, we apply genomic and microbiological methodologies to better understand iron acquisition via siderophores for environmental and respiratory isolates of M. abscessus, M. porcinum, and M. intracellulare subspecies chimaera from Hawai’i. Siderophore synthesis and transport genes, including mycobactin (mbt), mmpL/S, and esx-3 were compared among 47 reference isolates, 29 respiratory isolates, and 23 environmental Hawai’i isolates. Among all reference isolates examined, respiratory isolates showed significantly more siderophore pertinent genes compared to environmental isolates. Among the Hawai’i isolates, RGM M. abscessus and M. porcinum had significantly less esx-3 and mbt genes compared to SGM M. chimaera when stratified by growth classification. However, no significant differences were observed between the species when grown on low iron culture agar or siderophore production by the chrome azurol S (CAS) assay in vitro. These results indicate the complex mechanisms involved in iron sequestration and siderophore activity among diverse NTM species. Frontiers Media S.A. 2023-11-10 /pmc/articles/PMC10667711/ /pubmed/38029173 http://dx.doi.org/10.3389/fmicb.2023.1268963 Text en Copyright © 2023 Tan, Oberlag, McGowan, Dawrs, Chan, Strong, Hasan and Honda. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tan, Cara G.
Oberlag, Nicole M.
McGowan, Acelyn E.
Dawrs, Stephanie N.
Chan, Yvonne L.
Strong, Michael
Hasan, Nabeeh A.
Honda, Jennifer R.
Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i
title Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i
title_full Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i
title_fullStr Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i
title_full_unstemmed Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i
title_short Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i
title_sort genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from hawai’i
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667711/
https://www.ncbi.nlm.nih.gov/pubmed/38029173
http://dx.doi.org/10.3389/fmicb.2023.1268963
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