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Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples
Infections caused by extended-spectrum β-lactamase-producing Enterobacterales have increased rapidly and are mainly attributed to the production of CTX-M enzymes. This study evaluated the NG-Test(®) CTX-M MULTI lateral flow assay (CTX-M LFA) and the Rapid ESBL NP(®) test (ESBL NP test) for rapid det...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668757/ https://www.ncbi.nlm.nih.gov/pubmed/37998787 http://dx.doi.org/10.3390/antibiotics12111585 |
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author | Tang, Forrest Lee, Chung-Ho Li, Xin Jiang, Shuo Chow, Kin-Hung Tse, Cindy Wing-Sze Ho, Pak-Leung |
author_facet | Tang, Forrest Lee, Chung-Ho Li, Xin Jiang, Shuo Chow, Kin-Hung Tse, Cindy Wing-Sze Ho, Pak-Leung |
author_sort | Tang, Forrest |
collection | PubMed |
description | Infections caused by extended-spectrum β-lactamase-producing Enterobacterales have increased rapidly and are mainly attributed to the production of CTX-M enzymes. This study evaluated the NG-Test(®) CTX-M MULTI lateral flow assay (CTX-M LFA) and the Rapid ESBL NP(®) test (ESBL NP test) for rapid detection of CTX-M-producing Enterobacterales directly in midstream urine (MSU) samples. Testing was performed on 277 clinical MSU samples in a hospital microbiology laboratory from November 2022 to January 2023; 60 of these samples (30 positive for ESBL producers and 30 positive for non-ESBL producers) were tested retrospectively after the identification and susceptibility results were obtained, and 217 samples were tested prospectively immediately after a Gram stain showing the presence of Gram-negative bacilli. The results were compared against phenotypic detection of ESBL and molecular testing as the reference methods. Overall, 67 of the 277 samples were culture-positive for ESBL-producing Enterobacterales. PCR for the bla(CTX-M) gene was positive for all ESBL-producing Enterobacterales isolates. All CTX-M LFA results were interpretable, while three of the ESBL NP test results were noninterpretable. The sensitivity of the CTX-M LFA (100%, 95% CI 94.6–100%) was higher than that of the ESBL NP test (86.6%, 95% CI 76.0–93.7%). Both tests had high specificities (CTX-M LFA, 99.1%, 95% CI 96.6–99.9% and ESBL NP test, 100%, 95% CI 98.2–100%). In conclusion, both the CTX-M LFA and the ESBL NP test can deliver rapid results that could improve antimicrobial stewardship for urinary tract infections. |
format | Online Article Text |
id | pubmed-10668757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106687572023-11-02 Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples Tang, Forrest Lee, Chung-Ho Li, Xin Jiang, Shuo Chow, Kin-Hung Tse, Cindy Wing-Sze Ho, Pak-Leung Antibiotics (Basel) Article Infections caused by extended-spectrum β-lactamase-producing Enterobacterales have increased rapidly and are mainly attributed to the production of CTX-M enzymes. This study evaluated the NG-Test(®) CTX-M MULTI lateral flow assay (CTX-M LFA) and the Rapid ESBL NP(®) test (ESBL NP test) for rapid detection of CTX-M-producing Enterobacterales directly in midstream urine (MSU) samples. Testing was performed on 277 clinical MSU samples in a hospital microbiology laboratory from November 2022 to January 2023; 60 of these samples (30 positive for ESBL producers and 30 positive for non-ESBL producers) were tested retrospectively after the identification and susceptibility results were obtained, and 217 samples were tested prospectively immediately after a Gram stain showing the presence of Gram-negative bacilli. The results were compared against phenotypic detection of ESBL and molecular testing as the reference methods. Overall, 67 of the 277 samples were culture-positive for ESBL-producing Enterobacterales. PCR for the bla(CTX-M) gene was positive for all ESBL-producing Enterobacterales isolates. All CTX-M LFA results were interpretable, while three of the ESBL NP test results were noninterpretable. The sensitivity of the CTX-M LFA (100%, 95% CI 94.6–100%) was higher than that of the ESBL NP test (86.6%, 95% CI 76.0–93.7%). Both tests had high specificities (CTX-M LFA, 99.1%, 95% CI 96.6–99.9% and ESBL NP test, 100%, 95% CI 98.2–100%). In conclusion, both the CTX-M LFA and the ESBL NP test can deliver rapid results that could improve antimicrobial stewardship for urinary tract infections. MDPI 2023-11-02 /pmc/articles/PMC10668757/ /pubmed/37998787 http://dx.doi.org/10.3390/antibiotics12111585 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tang, Forrest Lee, Chung-Ho Li, Xin Jiang, Shuo Chow, Kin-Hung Tse, Cindy Wing-Sze Ho, Pak-Leung Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples |
title | Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples |
title_full | Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples |
title_fullStr | Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples |
title_full_unstemmed | Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples |
title_short | Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples |
title_sort | evaluation of two tests for the rapid detection of ctx-m producers directly in urine samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668757/ https://www.ncbi.nlm.nih.gov/pubmed/37998787 http://dx.doi.org/10.3390/antibiotics12111585 |
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