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Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 °C, and incorporated into a poly(dimeth...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668832/ https://www.ncbi.nlm.nih.gov/pubmed/37998822 http://dx.doi.org/10.3390/antibiotics12111620 |
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author | Gomes, Marisa Teixeira-Santos, Rita Gomes, Luciana C. Sousa-Cardoso, Francisca Carvalho, Fábio M. Tomé, Andreia R. Soares, Olívia S. G. P. Whitehead, Kathryn A. Mergulhão, Filipe J. |
author_facet | Gomes, Marisa Teixeira-Santos, Rita Gomes, Luciana C. Sousa-Cardoso, Francisca Carvalho, Fábio M. Tomé, Andreia R. Soares, Olívia S. G. P. Whitehead, Kathryn A. Mergulhão, Filipe J. |
author_sort | Gomes, Marisa |
collection | PubMed |
description | Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 °C, and incorporated into a poly(dimethylsiloxane) (PDMS) matrix. The materials’ textural properties were evaluated, and the roughness and morphology of MWCNT/PDMS composites were assessed using optical profilometry and scanning electron microscopy, respectively. The antibiofilm activity of MWCNT/PDMS surfaces was determined by quantifying culturable Escherichia coli and Staphylococcus aureus after 24 h of biofilm formation. Additionally, the antibacterial mechanisms of MWCNT materials were identified by flow cytometry, and the cytotoxicity of MWCNT/PDMS composites was tested against human kidney (HK-2) cells. The results revealed that the antimicrobial activity of MWCNTs incorporated into a PDMS matrix can be efficiently tailored through nitric acid functionalization, and it can be increased by up to 49% in the absence of surface carboxylic groups in f-MWCNT samples heated at 600 °C and the presence of redox activity of carbonyl groups. MWCNT materials changed the membrane permeability of both Gram-negative and Gram-positive bacteria, while they only induced the production of ROS in Gram-positive bacteria. Furthermore, the synthesized composites did not impact HK-2 cell viability, confirming the biocompatibility of MWCNT composites. |
format | Online Article Text |
id | pubmed-10668832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106688322023-11-11 Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus Gomes, Marisa Teixeira-Santos, Rita Gomes, Luciana C. Sousa-Cardoso, Francisca Carvalho, Fábio M. Tomé, Andreia R. Soares, Olívia S. G. P. Whitehead, Kathryn A. Mergulhão, Filipe J. Antibiotics (Basel) Article Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 °C, and incorporated into a poly(dimethylsiloxane) (PDMS) matrix. The materials’ textural properties were evaluated, and the roughness and morphology of MWCNT/PDMS composites were assessed using optical profilometry and scanning electron microscopy, respectively. The antibiofilm activity of MWCNT/PDMS surfaces was determined by quantifying culturable Escherichia coli and Staphylococcus aureus after 24 h of biofilm formation. Additionally, the antibacterial mechanisms of MWCNT materials were identified by flow cytometry, and the cytotoxicity of MWCNT/PDMS composites was tested against human kidney (HK-2) cells. The results revealed that the antimicrobial activity of MWCNTs incorporated into a PDMS matrix can be efficiently tailored through nitric acid functionalization, and it can be increased by up to 49% in the absence of surface carboxylic groups in f-MWCNT samples heated at 600 °C and the presence of redox activity of carbonyl groups. MWCNT materials changed the membrane permeability of both Gram-negative and Gram-positive bacteria, while they only induced the production of ROS in Gram-positive bacteria. Furthermore, the synthesized composites did not impact HK-2 cell viability, confirming the biocompatibility of MWCNT composites. MDPI 2023-11-11 /pmc/articles/PMC10668832/ /pubmed/37998822 http://dx.doi.org/10.3390/antibiotics12111620 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gomes, Marisa Teixeira-Santos, Rita Gomes, Luciana C. Sousa-Cardoso, Francisca Carvalho, Fábio M. Tomé, Andreia R. Soares, Olívia S. G. P. Whitehead, Kathryn A. Mergulhão, Filipe J. Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus |
title | Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus |
title_full | Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus |
title_fullStr | Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus |
title_full_unstemmed | Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus |
title_short | Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus |
title_sort | antibiofilm effect of nitric acid-functionalized carbon nanotube-based surfaces against e. coli and s. aureus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668832/ https://www.ncbi.nlm.nih.gov/pubmed/37998822 http://dx.doi.org/10.3390/antibiotics12111620 |
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