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Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus

Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 °C, and incorporated into a poly(dimeth...

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Autores principales: Gomes, Marisa, Teixeira-Santos, Rita, Gomes, Luciana C., Sousa-Cardoso, Francisca, Carvalho, Fábio M., Tomé, Andreia R., Soares, Olívia S. G. P., Whitehead, Kathryn A., Mergulhão, Filipe J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668832/
https://www.ncbi.nlm.nih.gov/pubmed/37998822
http://dx.doi.org/10.3390/antibiotics12111620
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author Gomes, Marisa
Teixeira-Santos, Rita
Gomes, Luciana C.
Sousa-Cardoso, Francisca
Carvalho, Fábio M.
Tomé, Andreia R.
Soares, Olívia S. G. P.
Whitehead, Kathryn A.
Mergulhão, Filipe J.
author_facet Gomes, Marisa
Teixeira-Santos, Rita
Gomes, Luciana C.
Sousa-Cardoso, Francisca
Carvalho, Fábio M.
Tomé, Andreia R.
Soares, Olívia S. G. P.
Whitehead, Kathryn A.
Mergulhão, Filipe J.
author_sort Gomes, Marisa
collection PubMed
description Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 °C, and incorporated into a poly(dimethylsiloxane) (PDMS) matrix. The materials’ textural properties were evaluated, and the roughness and morphology of MWCNT/PDMS composites were assessed using optical profilometry and scanning electron microscopy, respectively. The antibiofilm activity of MWCNT/PDMS surfaces was determined by quantifying culturable Escherichia coli and Staphylococcus aureus after 24 h of biofilm formation. Additionally, the antibacterial mechanisms of MWCNT materials were identified by flow cytometry, and the cytotoxicity of MWCNT/PDMS composites was tested against human kidney (HK-2) cells. The results revealed that the antimicrobial activity of MWCNTs incorporated into a PDMS matrix can be efficiently tailored through nitric acid functionalization, and it can be increased by up to 49% in the absence of surface carboxylic groups in f-MWCNT samples heated at 600 °C and the presence of redox activity of carbonyl groups. MWCNT materials changed the membrane permeability of both Gram-negative and Gram-positive bacteria, while they only induced the production of ROS in Gram-positive bacteria. Furthermore, the synthesized composites did not impact HK-2 cell viability, confirming the biocompatibility of MWCNT composites.
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spelling pubmed-106688322023-11-11 Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus Gomes, Marisa Teixeira-Santos, Rita Gomes, Luciana C. Sousa-Cardoso, Francisca Carvalho, Fábio M. Tomé, Andreia R. Soares, Olívia S. G. P. Whitehead, Kathryn A. Mergulhão, Filipe J. Antibiotics (Basel) Article Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 °C, and incorporated into a poly(dimethylsiloxane) (PDMS) matrix. The materials’ textural properties were evaluated, and the roughness and morphology of MWCNT/PDMS composites were assessed using optical profilometry and scanning electron microscopy, respectively. The antibiofilm activity of MWCNT/PDMS surfaces was determined by quantifying culturable Escherichia coli and Staphylococcus aureus after 24 h of biofilm formation. Additionally, the antibacterial mechanisms of MWCNT materials were identified by flow cytometry, and the cytotoxicity of MWCNT/PDMS composites was tested against human kidney (HK-2) cells. The results revealed that the antimicrobial activity of MWCNTs incorporated into a PDMS matrix can be efficiently tailored through nitric acid functionalization, and it can be increased by up to 49% in the absence of surface carboxylic groups in f-MWCNT samples heated at 600 °C and the presence of redox activity of carbonyl groups. MWCNT materials changed the membrane permeability of both Gram-negative and Gram-positive bacteria, while they only induced the production of ROS in Gram-positive bacteria. Furthermore, the synthesized composites did not impact HK-2 cell viability, confirming the biocompatibility of MWCNT composites. MDPI 2023-11-11 /pmc/articles/PMC10668832/ /pubmed/37998822 http://dx.doi.org/10.3390/antibiotics12111620 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gomes, Marisa
Teixeira-Santos, Rita
Gomes, Luciana C.
Sousa-Cardoso, Francisca
Carvalho, Fábio M.
Tomé, Andreia R.
Soares, Olívia S. G. P.
Whitehead, Kathryn A.
Mergulhão, Filipe J.
Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
title Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
title_full Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
title_fullStr Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
title_full_unstemmed Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
title_short Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
title_sort antibiofilm effect of nitric acid-functionalized carbon nanotube-based surfaces against e. coli and s. aureus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668832/
https://www.ncbi.nlm.nih.gov/pubmed/37998822
http://dx.doi.org/10.3390/antibiotics12111620
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