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Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens

While the lens is an avascular tissue with an immune-privileged status, studies have now revealed that there are immune responses specifically linked to the lens. The response to lens injury, such as following cataract surgery, has been shown to involve the activation of the resident immune cell pop...

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Autores principales: DeDreu, JodiRae, Basta, Morgan D., Walker, Janice L., Menko, A. Sue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668984/
https://www.ncbi.nlm.nih.gov/pubmed/38002297
http://dx.doi.org/10.3390/biom13111615
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author DeDreu, JodiRae
Basta, Morgan D.
Walker, Janice L.
Menko, A. Sue
author_facet DeDreu, JodiRae
Basta, Morgan D.
Walker, Janice L.
Menko, A. Sue
author_sort DeDreu, JodiRae
collection PubMed
description While the lens is an avascular tissue with an immune-privileged status, studies have now revealed that there are immune responses specifically linked to the lens. The response to lens injury, such as following cataract surgery, has been shown to involve the activation of the resident immune cell population of the lens and the induction of immunomodulatory factors by the wounded epithelium. However, there has been limited investigation into the immediate response of the lens to wounding, particularly those induced factors that are intrinsic to the lens and its associated resident immune cells. Using an established chick embryo ex vivo cataract surgery model has made it possible to determine the early immune responses of this tissue to injury, including its resident immune cells, through a transcriptome analysis. RNA-seq studies were performed to determine the gene expression profile at 1 h post wounding compared to time 0. The results provided evidence that, as occurs in other tissues, the resident immune cells of the lens rapidly acquired a molecular signature consistent with their activation. These studies also identified the expression of many inflammatory factors by the injured lens that are associated with both the induction and regulation of the immune response.
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spelling pubmed-106689842023-11-04 Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens DeDreu, JodiRae Basta, Morgan D. Walker, Janice L. Menko, A. Sue Biomolecules Article While the lens is an avascular tissue with an immune-privileged status, studies have now revealed that there are immune responses specifically linked to the lens. The response to lens injury, such as following cataract surgery, has been shown to involve the activation of the resident immune cell population of the lens and the induction of immunomodulatory factors by the wounded epithelium. However, there has been limited investigation into the immediate response of the lens to wounding, particularly those induced factors that are intrinsic to the lens and its associated resident immune cells. Using an established chick embryo ex vivo cataract surgery model has made it possible to determine the early immune responses of this tissue to injury, including its resident immune cells, through a transcriptome analysis. RNA-seq studies were performed to determine the gene expression profile at 1 h post wounding compared to time 0. The results provided evidence that, as occurs in other tissues, the resident immune cells of the lens rapidly acquired a molecular signature consistent with their activation. These studies also identified the expression of many inflammatory factors by the injured lens that are associated with both the induction and regulation of the immune response. MDPI 2023-11-04 /pmc/articles/PMC10668984/ /pubmed/38002297 http://dx.doi.org/10.3390/biom13111615 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
DeDreu, JodiRae
Basta, Morgan D.
Walker, Janice L.
Menko, A. Sue
Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens
title Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens
title_full Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens
title_fullStr Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens
title_full_unstemmed Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens
title_short Immune Responses Induced at One Hour Post Cataract Surgery Wounding of the Chick Lens
title_sort immune responses induced at one hour post cataract surgery wounding of the chick lens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668984/
https://www.ncbi.nlm.nih.gov/pubmed/38002297
http://dx.doi.org/10.3390/biom13111615
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