Cargando…
Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective
Quenchbody (Q-body) is a unique, reagentless, fluorescent antibody whose fluorescent intensity increases in an antigen-concentration-dependent manner. Q-body-based homogeneous immunoassay is superior to conventional immunoassays as it does not require multiple immobilization, reaction, and washing s...
Autor principal: | |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669387/ https://www.ncbi.nlm.nih.gov/pubmed/38002387 http://dx.doi.org/10.3390/bioengineering10111262 |
_version_ | 1785139684710547456 |
---|---|
author | Jeong, Hee-Jin |
author_facet | Jeong, Hee-Jin |
author_sort | Jeong, Hee-Jin |
collection | PubMed |
description | Quenchbody (Q-body) is a unique, reagentless, fluorescent antibody whose fluorescent intensity increases in an antigen-concentration-dependent manner. Q-body-based homogeneous immunoassay is superior to conventional immunoassays as it does not require multiple immobilization, reaction, and washing steps. In fact, simply mixing the Q-body and the sample containing the antigen enables the detection of the target antigen. To date, various Q-bodies have been developed to detect biomarkers of interest, including haptens, peptides, proteins, and cells. This review sought to describe the principle of Q-body-based immunoassay and the use of Q-body for various immunoassays. In particular, the Q-bodies were classified from a structural perspective to provide useful information for designing Q-bodies with an appropriate objective. |
format | Online Article Text |
id | pubmed-10669387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106693872023-10-30 Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective Jeong, Hee-Jin Bioengineering (Basel) Review Quenchbody (Q-body) is a unique, reagentless, fluorescent antibody whose fluorescent intensity increases in an antigen-concentration-dependent manner. Q-body-based homogeneous immunoassay is superior to conventional immunoassays as it does not require multiple immobilization, reaction, and washing steps. In fact, simply mixing the Q-body and the sample containing the antigen enables the detection of the target antigen. To date, various Q-bodies have been developed to detect biomarkers of interest, including haptens, peptides, proteins, and cells. This review sought to describe the principle of Q-body-based immunoassay and the use of Q-body for various immunoassays. In particular, the Q-bodies were classified from a structural perspective to provide useful information for designing Q-bodies with an appropriate objective. MDPI 2023-10-30 /pmc/articles/PMC10669387/ /pubmed/38002387 http://dx.doi.org/10.3390/bioengineering10111262 Text en © 2023 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Jeong, Hee-Jin Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
title | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
title_full | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
title_fullStr | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
title_full_unstemmed | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
title_short | Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective |
title_sort | quenchbodies that enable one-pot detection of antigens: a structural perspective |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669387/ https://www.ncbi.nlm.nih.gov/pubmed/38002387 http://dx.doi.org/10.3390/bioengineering10111262 |
work_keys_str_mv | AT jeongheejin quenchbodiesthatenableonepotdetectionofantigensastructuralperspective |