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High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor
In order to facilitate monitoring of cannabidiol (CBD), we devised a gold immunochromatographic sensor based on a specific monoclonal antibody (mAb). To prepare the antigen, a novel hapten with CBD moiety and a linear carbon chain was employed. By utilizing hybridoma technology, a specific mAb was s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669437/ https://www.ncbi.nlm.nih.gov/pubmed/37998135 http://dx.doi.org/10.3390/bios13110960 |
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author | Sun, Yufeng Zhu, Dong Tao, Ran Li, Long Fan, Bei Wang, Fengzhong |
author_facet | Sun, Yufeng Zhu, Dong Tao, Ran Li, Long Fan, Bei Wang, Fengzhong |
author_sort | Sun, Yufeng |
collection | PubMed |
description | In order to facilitate monitoring of cannabidiol (CBD), we devised a gold immunochromatographic sensor based on a specific monoclonal antibody (mAb). To prepare the antigen, a novel hapten with CBD moiety and a linear carbon chain was employed. By utilizing hybridoma technology, a specific mAb was screened and identified that exhibited a 50% maximal inhibitory concentration against CBD ranging from 28.97 to 443.97 ng/mL. Extensive optimization led to the establishment of visual limits of detection for CBD, achieving a remarkable sensitivity of 8 μg/mL in the assay buffer. To showcase the accuracy and stability, an analysis of CBD-spiked wine, sparkling water, and sports drink was conducted. The recovery rates observed were as follows: 88.4–109.2% for wine, 89.9–107.8% for sparkling water, and 83.2–95.5% for sports drink. Furthermore, the coefficient of variation remained impressively low, less than 4.38% for wine, less than 2.07% for sparkling water, and less than 6.34% for sports drink. Importantly, the developed sensor exhibited no cross-reaction with tetrahydrocannabinol (THC). In conclusion, the proposed paper sensor, employing gold nanoparticles, offers a user-friendly and efficient approach for the precise, rapid, and dependable determination of CBD in products. |
format | Online Article Text |
id | pubmed-10669437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106694372023-10-28 High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor Sun, Yufeng Zhu, Dong Tao, Ran Li, Long Fan, Bei Wang, Fengzhong Biosensors (Basel) Article In order to facilitate monitoring of cannabidiol (CBD), we devised a gold immunochromatographic sensor based on a specific monoclonal antibody (mAb). To prepare the antigen, a novel hapten with CBD moiety and a linear carbon chain was employed. By utilizing hybridoma technology, a specific mAb was screened and identified that exhibited a 50% maximal inhibitory concentration against CBD ranging from 28.97 to 443.97 ng/mL. Extensive optimization led to the establishment of visual limits of detection for CBD, achieving a remarkable sensitivity of 8 μg/mL in the assay buffer. To showcase the accuracy and stability, an analysis of CBD-spiked wine, sparkling water, and sports drink was conducted. The recovery rates observed were as follows: 88.4–109.2% for wine, 89.9–107.8% for sparkling water, and 83.2–95.5% for sports drink. Furthermore, the coefficient of variation remained impressively low, less than 4.38% for wine, less than 2.07% for sparkling water, and less than 6.34% for sports drink. Importantly, the developed sensor exhibited no cross-reaction with tetrahydrocannabinol (THC). In conclusion, the proposed paper sensor, employing gold nanoparticles, offers a user-friendly and efficient approach for the precise, rapid, and dependable determination of CBD in products. MDPI 2023-10-28 /pmc/articles/PMC10669437/ /pubmed/37998135 http://dx.doi.org/10.3390/bios13110960 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sun, Yufeng Zhu, Dong Tao, Ran Li, Long Fan, Bei Wang, Fengzhong High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor |
title | High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor |
title_full | High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor |
title_fullStr | High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor |
title_full_unstemmed | High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor |
title_short | High Specific and Rapid Detection of Cannabidiol by Gold Nanoparticle-Based Paper Sensor |
title_sort | high specific and rapid detection of cannabidiol by gold nanoparticle-based paper sensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669437/ https://www.ncbi.nlm.nih.gov/pubmed/37998135 http://dx.doi.org/10.3390/bios13110960 |
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