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Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days

This study aimed to analyse and compare the vancomycin elution kinetics of four biodegradable, osteoconductive antibiotic carriers used in clinical practice within a 42-day in vitro setting. Carriers A and D already contained vancomycin (1.1 g and 0.247 g), whereas carriers B and C were mixed with v...

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Autores principales: Smolle, Maria Anna, Murtezai, Hana, Niedrist, Tobias, Amerstorfer, Florian, Hörlesberger, Nina, Leitner, Lukas, Klim, Sebastian Martin, Glehr, Reingard, Ahluwalia, Raju, Leithner, Andreas, Glehr, Mathias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669465/
https://www.ncbi.nlm.nih.gov/pubmed/37998838
http://dx.doi.org/10.3390/antibiotics12111636
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author Smolle, Maria Anna
Murtezai, Hana
Niedrist, Tobias
Amerstorfer, Florian
Hörlesberger, Nina
Leitner, Lukas
Klim, Sebastian Martin
Glehr, Reingard
Ahluwalia, Raju
Leithner, Andreas
Glehr, Mathias
author_facet Smolle, Maria Anna
Murtezai, Hana
Niedrist, Tobias
Amerstorfer, Florian
Hörlesberger, Nina
Leitner, Lukas
Klim, Sebastian Martin
Glehr, Reingard
Ahluwalia, Raju
Leithner, Andreas
Glehr, Mathias
author_sort Smolle, Maria Anna
collection PubMed
description This study aimed to analyse and compare the vancomycin elution kinetics of four biodegradable, osteoconductive antibiotic carriers used in clinical practice within a 42-day in vitro setting. Carriers A and D already contained vancomycin (1.1 g and 0.247 g), whereas carriers B and C were mixed with vancomycin according to the manufacturer’s recommendations (B: 0.83 g and C: 0.305 g). At nine time points, 50% (4.5 mL) of the elution sample was removed and substituted with the same amount of PBS. Probes were analysed with a kinetic microparticle immunoassay. Time-dependent changes in vancomycin concentrations for each carrier and differences between carriers were analysed. Mean initial antibiotic levels were highest for carrier A (37.5 mg/mL) and lowest for carrier B (5.4 mg/mL). We observed time-dependent, strongly negative linear elution kinetics for carriers A (−0.835; p < 0.001), C (−0.793; p < 0.001), and D (−0.853; p < 0.001). Vancomycin concentrations increased from 48 h to 7 d and dropped thereafter in carriers C and D whilst constantly decreasing at any time point for carrier A. Carrier B showed a shallower decrease. Mean antibiotics levels at 42 d were 1.5 mg/mL, 2.6 mg/mL, 0.1 mg/mL, and 0.1 mg/mL for carriers A, B, C, and D. Differences in mean initial and final vancomycin concentrations for carrier A were significantly larger in comparison to C (p = 0.040). A carrier consisting of allogenic bone chips showed the highest vancomycin-to-carrier ratio and the largest elution over the study period. Whilst vancomycin concentrations were still measurable at 42 days for all carriers, carrier A provided a higher drug-to-carrier ratio and a more consistent antibiotic-releasing profile.
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spelling pubmed-106694652023-11-17 Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days Smolle, Maria Anna Murtezai, Hana Niedrist, Tobias Amerstorfer, Florian Hörlesberger, Nina Leitner, Lukas Klim, Sebastian Martin Glehr, Reingard Ahluwalia, Raju Leithner, Andreas Glehr, Mathias Antibiotics (Basel) Article This study aimed to analyse and compare the vancomycin elution kinetics of four biodegradable, osteoconductive antibiotic carriers used in clinical practice within a 42-day in vitro setting. Carriers A and D already contained vancomycin (1.1 g and 0.247 g), whereas carriers B and C were mixed with vancomycin according to the manufacturer’s recommendations (B: 0.83 g and C: 0.305 g). At nine time points, 50% (4.5 mL) of the elution sample was removed and substituted with the same amount of PBS. Probes were analysed with a kinetic microparticle immunoassay. Time-dependent changes in vancomycin concentrations for each carrier and differences between carriers were analysed. Mean initial antibiotic levels were highest for carrier A (37.5 mg/mL) and lowest for carrier B (5.4 mg/mL). We observed time-dependent, strongly negative linear elution kinetics for carriers A (−0.835; p < 0.001), C (−0.793; p < 0.001), and D (−0.853; p < 0.001). Vancomycin concentrations increased from 48 h to 7 d and dropped thereafter in carriers C and D whilst constantly decreasing at any time point for carrier A. Carrier B showed a shallower decrease. Mean antibiotics levels at 42 d were 1.5 mg/mL, 2.6 mg/mL, 0.1 mg/mL, and 0.1 mg/mL for carriers A, B, C, and D. Differences in mean initial and final vancomycin concentrations for carrier A were significantly larger in comparison to C (p = 0.040). A carrier consisting of allogenic bone chips showed the highest vancomycin-to-carrier ratio and the largest elution over the study period. Whilst vancomycin concentrations were still measurable at 42 days for all carriers, carrier A provided a higher drug-to-carrier ratio and a more consistent antibiotic-releasing profile. MDPI 2023-11-17 /pmc/articles/PMC10669465/ /pubmed/37998838 http://dx.doi.org/10.3390/antibiotics12111636 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Smolle, Maria Anna
Murtezai, Hana
Niedrist, Tobias
Amerstorfer, Florian
Hörlesberger, Nina
Leitner, Lukas
Klim, Sebastian Martin
Glehr, Reingard
Ahluwalia, Raju
Leithner, Andreas
Glehr, Mathias
Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days
title Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days
title_full Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days
title_fullStr Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days
title_full_unstemmed Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days
title_short Vancomycin Elution Kinetics of Four Antibiotic Carriers Used in Orthopaedic Surgery: In Vitro Study over 42 Days
title_sort vancomycin elution kinetics of four antibiotic carriers used in orthopaedic surgery: in vitro study over 42 days
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669465/
https://www.ncbi.nlm.nih.gov/pubmed/37998838
http://dx.doi.org/10.3390/antibiotics12111636
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