Cargando…

pH Regulator on Digital Microfluidics with Pico-Dosing Technique

Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is prese...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Haoran, Peng, Tao, Zhong, Yunlong, Liu, Meiqing, Mak, Pui-In, Martins, Rui P., Wang, Ping, Jia, Yanwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669492/
https://www.ncbi.nlm.nih.gov/pubmed/37998126
http://dx.doi.org/10.3390/bios13110951
Descripción
Sumario:Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is presented to deliver alkali/acid into the sample solution to change the pH value of the sample. An image analysis method based on ImageJ is developed to calculate the delivered volume and an on-chip colorimetric method is proposed to determine the pH value of the sample solution containing the acid-base indicator. The calculated pH values show consistency with the measured ones. Our approach makes the real-time pH control of the on-chip biological experiment more easy to control and flexible.