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pH Regulator on Digital Microfluidics with Pico-Dosing Technique

Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is prese...

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Detalles Bibliográficos
Autores principales: Li, Haoran, Peng, Tao, Zhong, Yunlong, Liu, Meiqing, Mak, Pui-In, Martins, Rui P., Wang, Ping, Jia, Yanwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669492/
https://www.ncbi.nlm.nih.gov/pubmed/37998126
http://dx.doi.org/10.3390/bios13110951
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author Li, Haoran
Peng, Tao
Zhong, Yunlong
Liu, Meiqing
Mak, Pui-In
Martins, Rui P.
Wang, Ping
Jia, Yanwei
author_facet Li, Haoran
Peng, Tao
Zhong, Yunlong
Liu, Meiqing
Mak, Pui-In
Martins, Rui P.
Wang, Ping
Jia, Yanwei
author_sort Li, Haoran
collection PubMed
description Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is presented to deliver alkali/acid into the sample solution to change the pH value of the sample. An image analysis method based on ImageJ is developed to calculate the delivered volume and an on-chip colorimetric method is proposed to determine the pH value of the sample solution containing the acid-base indicator. The calculated pH values show consistency with the measured ones. Our approach makes the real-time pH control of the on-chip biological experiment more easy to control and flexible.
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spelling pubmed-106694922023-10-25 pH Regulator on Digital Microfluidics with Pico-Dosing Technique Li, Haoran Peng, Tao Zhong, Yunlong Liu, Meiqing Mak, Pui-In Martins, Rui P. Wang, Ping Jia, Yanwei Biosensors (Basel) Article Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is presented to deliver alkali/acid into the sample solution to change the pH value of the sample. An image analysis method based on ImageJ is developed to calculate the delivered volume and an on-chip colorimetric method is proposed to determine the pH value of the sample solution containing the acid-base indicator. The calculated pH values show consistency with the measured ones. Our approach makes the real-time pH control of the on-chip biological experiment more easy to control and flexible. MDPI 2023-10-25 /pmc/articles/PMC10669492/ /pubmed/37998126 http://dx.doi.org/10.3390/bios13110951 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Haoran
Peng, Tao
Zhong, Yunlong
Liu, Meiqing
Mak, Pui-In
Martins, Rui P.
Wang, Ping
Jia, Yanwei
pH Regulator on Digital Microfluidics with Pico-Dosing Technique
title pH Regulator on Digital Microfluidics with Pico-Dosing Technique
title_full pH Regulator on Digital Microfluidics with Pico-Dosing Technique
title_fullStr pH Regulator on Digital Microfluidics with Pico-Dosing Technique
title_full_unstemmed pH Regulator on Digital Microfluidics with Pico-Dosing Technique
title_short pH Regulator on Digital Microfluidics with Pico-Dosing Technique
title_sort ph regulator on digital microfluidics with pico-dosing technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669492/
https://www.ncbi.nlm.nih.gov/pubmed/37998126
http://dx.doi.org/10.3390/bios13110951
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