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pH Regulator on Digital Microfluidics with Pico-Dosing Technique
Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is prese...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669492/ https://www.ncbi.nlm.nih.gov/pubmed/37998126 http://dx.doi.org/10.3390/bios13110951 |
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author | Li, Haoran Peng, Tao Zhong, Yunlong Liu, Meiqing Mak, Pui-In Martins, Rui P. Wang, Ping Jia, Yanwei |
author_facet | Li, Haoran Peng, Tao Zhong, Yunlong Liu, Meiqing Mak, Pui-In Martins, Rui P. Wang, Ping Jia, Yanwei |
author_sort | Li, Haoran |
collection | PubMed |
description | Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is presented to deliver alkali/acid into the sample solution to change the pH value of the sample. An image analysis method based on ImageJ is developed to calculate the delivered volume and an on-chip colorimetric method is proposed to determine the pH value of the sample solution containing the acid-base indicator. The calculated pH values show consistency with the measured ones. Our approach makes the real-time pH control of the on-chip biological experiment more easy to control and flexible. |
format | Online Article Text |
id | pubmed-10669492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106694922023-10-25 pH Regulator on Digital Microfluidics with Pico-Dosing Technique Li, Haoran Peng, Tao Zhong, Yunlong Liu, Meiqing Mak, Pui-In Martins, Rui P. Wang, Ping Jia, Yanwei Biosensors (Basel) Article Real-time pH control on-chip is a crucial factor for cell-based experiments in microfluidics, yet difficult to realize. In this paper, we present a flexible pH regulator on a digital microfluidic (DMF) platform. The pico-dosing technology, which can generate and transfer satellite droplets, is presented to deliver alkali/acid into the sample solution to change the pH value of the sample. An image analysis method based on ImageJ is developed to calculate the delivered volume and an on-chip colorimetric method is proposed to determine the pH value of the sample solution containing the acid-base indicator. The calculated pH values show consistency with the measured ones. Our approach makes the real-time pH control of the on-chip biological experiment more easy to control and flexible. MDPI 2023-10-25 /pmc/articles/PMC10669492/ /pubmed/37998126 http://dx.doi.org/10.3390/bios13110951 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Haoran Peng, Tao Zhong, Yunlong Liu, Meiqing Mak, Pui-In Martins, Rui P. Wang, Ping Jia, Yanwei pH Regulator on Digital Microfluidics with Pico-Dosing Technique |
title | pH Regulator on Digital Microfluidics with Pico-Dosing Technique |
title_full | pH Regulator on Digital Microfluidics with Pico-Dosing Technique |
title_fullStr | pH Regulator on Digital Microfluidics with Pico-Dosing Technique |
title_full_unstemmed | pH Regulator on Digital Microfluidics with Pico-Dosing Technique |
title_short | pH Regulator on Digital Microfluidics with Pico-Dosing Technique |
title_sort | ph regulator on digital microfluidics with pico-dosing technique |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669492/ https://www.ncbi.nlm.nih.gov/pubmed/37998126 http://dx.doi.org/10.3390/bios13110951 |
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