Cargando…

LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1

Glycoproteomic analysis is always challenging because of low abundance and complex site-specific heterogeneity. Glycoproteins are involved in various biological processes such as cell signaling, adhesion, and cell–cell communication and may serve as potential biomarkers when analyzing different dise...

Descripción completa

Detalles Bibliográficos
Autores principales: Atashi, Mojgan, Reyes, Cristian D. Gutierrez, Sandilya, Vishal, Purba, Waziha, Ahmadi, Parisa, Hakim, Md. Abdul, Kobeissy, Firas, Plazzi, Giuseppe, Moresco, Monica, Lanuzza, Bartolo, Ferri, Raffaele, Mechref, Yehia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669497/
https://www.ncbi.nlm.nih.gov/pubmed/38002271
http://dx.doi.org/10.3390/biom13111589
_version_ 1785139710779195392
author Atashi, Mojgan
Reyes, Cristian D. Gutierrez
Sandilya, Vishal
Purba, Waziha
Ahmadi, Parisa
Hakim, Md. Abdul
Kobeissy, Firas
Plazzi, Giuseppe
Moresco, Monica
Lanuzza, Bartolo
Ferri, Raffaele
Mechref, Yehia
author_facet Atashi, Mojgan
Reyes, Cristian D. Gutierrez
Sandilya, Vishal
Purba, Waziha
Ahmadi, Parisa
Hakim, Md. Abdul
Kobeissy, Firas
Plazzi, Giuseppe
Moresco, Monica
Lanuzza, Bartolo
Ferri, Raffaele
Mechref, Yehia
author_sort Atashi, Mojgan
collection PubMed
description Glycoproteomic analysis is always challenging because of low abundance and complex site-specific heterogeneity. Glycoproteins are involved in various biological processes such as cell signaling, adhesion, and cell–cell communication and may serve as potential biomarkers when analyzing different diseases. Here, we investigate glycoproteins in narcolepsy type 1 (NT1) disease, a form of narcolepsy characterized by cataplexy—the sudden onset of muscle paralysis that is typically triggered by intense emotions. In this study, 27 human blood serum samples were analyzed, 16 from NT1 patients and 11 from healthy individuals serving as controls. We quantified hydrophilic interaction liquid chromatography (HILIC)-enriched glycopeptides from low-abundance serum samples of controls and NT1 patients via LC-MS/MS. Twenty-eight unique N-glycopeptides showed significant changes between the two studied groups. The sialylated N-glycopeptide structures LPTQNITFQTESSVAEQEAEFQSPK HexNAc(6), Hex(3), Neu5Ac(2) (derived from the ITIH4 protein) and the structure IVLDPSGSMNIYLVLDGSDSIGASNFTGAK HexNAc(5), Hex(4), Fuc(1) (derived from the CFB protein), with p values of 0.008 and 0.01, respectively, were elevated in NT1 samples compared with controls. In addition, the N-glycopeptide protein sources Ceruloplasmin, Complement factor B, and ITH4 were observed to play an important role in the complement activation and acute-phase response signaling pathways. This may explain the possible association between the biomarkers and pathophysiological effects.
format Online
Article
Text
id pubmed-10669497
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106694972023-10-28 LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1 Atashi, Mojgan Reyes, Cristian D. Gutierrez Sandilya, Vishal Purba, Waziha Ahmadi, Parisa Hakim, Md. Abdul Kobeissy, Firas Plazzi, Giuseppe Moresco, Monica Lanuzza, Bartolo Ferri, Raffaele Mechref, Yehia Biomolecules Article Glycoproteomic analysis is always challenging because of low abundance and complex site-specific heterogeneity. Glycoproteins are involved in various biological processes such as cell signaling, adhesion, and cell–cell communication and may serve as potential biomarkers when analyzing different diseases. Here, we investigate glycoproteins in narcolepsy type 1 (NT1) disease, a form of narcolepsy characterized by cataplexy—the sudden onset of muscle paralysis that is typically triggered by intense emotions. In this study, 27 human blood serum samples were analyzed, 16 from NT1 patients and 11 from healthy individuals serving as controls. We quantified hydrophilic interaction liquid chromatography (HILIC)-enriched glycopeptides from low-abundance serum samples of controls and NT1 patients via LC-MS/MS. Twenty-eight unique N-glycopeptides showed significant changes between the two studied groups. The sialylated N-glycopeptide structures LPTQNITFQTESSVAEQEAEFQSPK HexNAc(6), Hex(3), Neu5Ac(2) (derived from the ITIH4 protein) and the structure IVLDPSGSMNIYLVLDGSDSIGASNFTGAK HexNAc(5), Hex(4), Fuc(1) (derived from the CFB protein), with p values of 0.008 and 0.01, respectively, were elevated in NT1 samples compared with controls. In addition, the N-glycopeptide protein sources Ceruloplasmin, Complement factor B, and ITH4 were observed to play an important role in the complement activation and acute-phase response signaling pathways. This may explain the possible association between the biomarkers and pathophysiological effects. MDPI 2023-10-28 /pmc/articles/PMC10669497/ /pubmed/38002271 http://dx.doi.org/10.3390/biom13111589 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Atashi, Mojgan
Reyes, Cristian D. Gutierrez
Sandilya, Vishal
Purba, Waziha
Ahmadi, Parisa
Hakim, Md. Abdul
Kobeissy, Firas
Plazzi, Giuseppe
Moresco, Monica
Lanuzza, Bartolo
Ferri, Raffaele
Mechref, Yehia
LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1
title LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1
title_full LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1
title_fullStr LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1
title_full_unstemmed LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1
title_short LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1
title_sort lc-ms/ms quantitation of hilic-enriched n-glycopeptides derived from low-abundance serum glycoproteins in patients with narcolepsy type 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669497/
https://www.ncbi.nlm.nih.gov/pubmed/38002271
http://dx.doi.org/10.3390/biom13111589
work_keys_str_mv AT atashimojgan lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT reyescristiandgutierrez lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT sandilyavishal lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT purbawaziha lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT ahmadiparisa lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT hakimmdabdul lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT kobeissyfiras lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT plazzigiuseppe lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT morescomonica lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT lanuzzabartolo lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT ferriraffaele lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1
AT mechrefyehia lcmsmsquantitationofhilicenrichednglycopeptidesderivedfromlowabundanceserumglycoproteinsinpatientswithnarcolepsytype1