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Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin
(1) Background: Inflammatory responses induce the formation of both anti-tumor and pro-tumor neutrophils known as myeloid-derived suppressor cells (MDSCs). Intermittent intravesical infusion of Bacillus Calmette–Guérin (BCG) is an established cancer immunotherapy for non-muscle-invasive bladder canc...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669614/ https://www.ncbi.nlm.nih.gov/pubmed/38002062 http://dx.doi.org/10.3390/biomedicines11113062 |
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author | Takeda, Yuji Kato, Tomoyuki Sabrina, Saima Naito, Sei Ito, Hiromi Emi, Naoto Kuboki, Yuya Takai, Yuki Fukuhara, Hiroki Ushijima, Masaki Narisawa, Takafumi Yagi, Mayu Kanno, Hidenori Sakurai, Toshihiko Nishida, Hayato Araki, Akemi Shimotai, Yoshitaka Nagashima, Mikako Nouchi, Yusuke Saitoh, Shinichi Nara, Hidetoshi Tsuchiya, Norihiko Asao, Hironobu |
author_facet | Takeda, Yuji Kato, Tomoyuki Sabrina, Saima Naito, Sei Ito, Hiromi Emi, Naoto Kuboki, Yuya Takai, Yuki Fukuhara, Hiroki Ushijima, Masaki Narisawa, Takafumi Yagi, Mayu Kanno, Hidenori Sakurai, Toshihiko Nishida, Hayato Araki, Akemi Shimotai, Yoshitaka Nagashima, Mikako Nouchi, Yusuke Saitoh, Shinichi Nara, Hidetoshi Tsuchiya, Norihiko Asao, Hironobu |
author_sort | Takeda, Yuji |
collection | PubMed |
description | (1) Background: Inflammatory responses induce the formation of both anti-tumor and pro-tumor neutrophils known as myeloid-derived suppressor cells (MDSCs). Intermittent intravesical infusion of Bacillus Calmette–Guérin (BCG) is an established cancer immunotherapy for non-muscle-invasive bladder cancer (NMIBC). However, the types of neutrophils induced via the inflammatory response to both tumor-bearing and BCG remain unclear. (2) Methods: We therefore analyzed neutrophil dynamics in the peripheral blood and urine of patients with NMIBC who received BCG therapy. Further, we analyzed the effects of BCG in a mouse intraperitoneal tumor model. (3) Results: BCG therapy induced the formation of CXCL10 and MHC class II-positive neutrophils in the urine of patients with NMIBC but did not induce MDSC formation. CXCL10- and MHC class II-expressing neutrophils were detected in peritoneal exudate cells formed after BCG administration. Partial neutrophil depletion using an anti-Ly6G antibody suppressed the upregulation of CXCL10 and MHC class II in neutrophils and reversed the anti-tumor activity of BCG in mouse models. (4) Conclusions: These results indicated that intracellular MHC class II- and CXCL10-expressing neutrophils indicate the state of anti-tumor activity induced via BCG. The status of neutrophils in mixed inflammation of immunosuppressive and anti-tumor responses may therefore be useful for evaluating immunological systemic conditions. |
format | Online Article Text |
id | pubmed-10669614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106696142023-11-15 Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin Takeda, Yuji Kato, Tomoyuki Sabrina, Saima Naito, Sei Ito, Hiromi Emi, Naoto Kuboki, Yuya Takai, Yuki Fukuhara, Hiroki Ushijima, Masaki Narisawa, Takafumi Yagi, Mayu Kanno, Hidenori Sakurai, Toshihiko Nishida, Hayato Araki, Akemi Shimotai, Yoshitaka Nagashima, Mikako Nouchi, Yusuke Saitoh, Shinichi Nara, Hidetoshi Tsuchiya, Norihiko Asao, Hironobu Biomedicines Article (1) Background: Inflammatory responses induce the formation of both anti-tumor and pro-tumor neutrophils known as myeloid-derived suppressor cells (MDSCs). Intermittent intravesical infusion of Bacillus Calmette–Guérin (BCG) is an established cancer immunotherapy for non-muscle-invasive bladder cancer (NMIBC). However, the types of neutrophils induced via the inflammatory response to both tumor-bearing and BCG remain unclear. (2) Methods: We therefore analyzed neutrophil dynamics in the peripheral blood and urine of patients with NMIBC who received BCG therapy. Further, we analyzed the effects of BCG in a mouse intraperitoneal tumor model. (3) Results: BCG therapy induced the formation of CXCL10 and MHC class II-positive neutrophils in the urine of patients with NMIBC but did not induce MDSC formation. CXCL10- and MHC class II-expressing neutrophils were detected in peritoneal exudate cells formed after BCG administration. Partial neutrophil depletion using an anti-Ly6G antibody suppressed the upregulation of CXCL10 and MHC class II in neutrophils and reversed the anti-tumor activity of BCG in mouse models. (4) Conclusions: These results indicated that intracellular MHC class II- and CXCL10-expressing neutrophils indicate the state of anti-tumor activity induced via BCG. The status of neutrophils in mixed inflammation of immunosuppressive and anti-tumor responses may therefore be useful for evaluating immunological systemic conditions. MDPI 2023-11-15 /pmc/articles/PMC10669614/ /pubmed/38002062 http://dx.doi.org/10.3390/biomedicines11113062 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Takeda, Yuji Kato, Tomoyuki Sabrina, Saima Naito, Sei Ito, Hiromi Emi, Naoto Kuboki, Yuya Takai, Yuki Fukuhara, Hiroki Ushijima, Masaki Narisawa, Takafumi Yagi, Mayu Kanno, Hidenori Sakurai, Toshihiko Nishida, Hayato Araki, Akemi Shimotai, Yoshitaka Nagashima, Mikako Nouchi, Yusuke Saitoh, Shinichi Nara, Hidetoshi Tsuchiya, Norihiko Asao, Hironobu Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin |
title | Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin |
title_full | Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin |
title_fullStr | Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin |
title_full_unstemmed | Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin |
title_short | Intracellular Major Histocompatibility Complex Class II and C-X-C Motif Chemokine Ligand 10-Expressing Neutrophils Indicate the State of Anti-Tumor Activity Induced by Bacillus Calmette–Guérin |
title_sort | intracellular major histocompatibility complex class ii and c-x-c motif chemokine ligand 10-expressing neutrophils indicate the state of anti-tumor activity induced by bacillus calmette–guérin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669614/ https://www.ncbi.nlm.nih.gov/pubmed/38002062 http://dx.doi.org/10.3390/biomedicines11113062 |
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