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Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate

Plasma gel (PG) is a blood-derived biomaterial that can be prepared by heating or chemical cross-linking without the aid of intrinsic coagulation activity and has gradually been applied in the field of esthetic surgery. To explore the applicability of PG in regenerative therapy or tissue engineering...

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Autores principales: Nakamura, Masayuki, Masuki, Hideo, Kawabata, Hideo, Watanabe, Taisuke, Watanabe, Takao, Tsujino, Tetsuhiro, Isobe, Kazushige, Kitamura, Yutaka, Mourão, Carlos Fernando, Kawase, Tomoyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669793/
https://www.ncbi.nlm.nih.gov/pubmed/38001872
http://dx.doi.org/10.3390/biomedicines11112871
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author Nakamura, Masayuki
Masuki, Hideo
Kawabata, Hideo
Watanabe, Taisuke
Watanabe, Takao
Tsujino, Tetsuhiro
Isobe, Kazushige
Kitamura, Yutaka
Mourão, Carlos Fernando
Kawase, Tomoyuki
author_facet Nakamura, Masayuki
Masuki, Hideo
Kawabata, Hideo
Watanabe, Taisuke
Watanabe, Takao
Tsujino, Tetsuhiro
Isobe, Kazushige
Kitamura, Yutaka
Mourão, Carlos Fernando
Kawase, Tomoyuki
author_sort Nakamura, Masayuki
collection PubMed
description Plasma gel (PG) is a blood-derived biomaterial that can be prepared by heating or chemical cross-linking without the aid of intrinsic coagulation activity and has gradually been applied in the field of esthetic surgery. To explore the applicability of PG in regenerative therapy or tissue engineering, in this study, we focused on the advantages of the heating method and verified the retention capacity of the resulting PG for polyphosphate (polyP), a polyanion that contributes to hemostasis and bone regeneration. Pooled platelet-poor plasma (PPP) was prepared from four healthy male adult donors, mixed with synthetic polyP, and heated at 75 °C for 10 or 30 min to prepare PG in microtubes. The PG was incubated in PBS at 37 °C, and polyP levels in the extra-matrix PBS were determined by the fluorometric method every 24 h. The microstructure of PG was examined using scanning electron microscopy. In the small PG matrices, almost all of the added polyP (~100%) was released within the initial 24 h. In contrast, in the large PG matrices, approximately 50% of the polyP was released within the initial 24 h and thereafter gradually released over time. Owing to its simple chemical structure, linear polyP cannot be theoretically retained in the gel matrices used in this study. However, these findings suggest that thermally prepared PG matrices can be applied as carriers of polyP in tissue engineering and regenerative medicine.
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spelling pubmed-106697932023-10-24 Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate Nakamura, Masayuki Masuki, Hideo Kawabata, Hideo Watanabe, Taisuke Watanabe, Takao Tsujino, Tetsuhiro Isobe, Kazushige Kitamura, Yutaka Mourão, Carlos Fernando Kawase, Tomoyuki Biomedicines Article Plasma gel (PG) is a blood-derived biomaterial that can be prepared by heating or chemical cross-linking without the aid of intrinsic coagulation activity and has gradually been applied in the field of esthetic surgery. To explore the applicability of PG in regenerative therapy or tissue engineering, in this study, we focused on the advantages of the heating method and verified the retention capacity of the resulting PG for polyphosphate (polyP), a polyanion that contributes to hemostasis and bone regeneration. Pooled platelet-poor plasma (PPP) was prepared from four healthy male adult donors, mixed with synthetic polyP, and heated at 75 °C for 10 or 30 min to prepare PG in microtubes. The PG was incubated in PBS at 37 °C, and polyP levels in the extra-matrix PBS were determined by the fluorometric method every 24 h. The microstructure of PG was examined using scanning electron microscopy. In the small PG matrices, almost all of the added polyP (~100%) was released within the initial 24 h. In contrast, in the large PG matrices, approximately 50% of the polyP was released within the initial 24 h and thereafter gradually released over time. Owing to its simple chemical structure, linear polyP cannot be theoretically retained in the gel matrices used in this study. However, these findings suggest that thermally prepared PG matrices can be applied as carriers of polyP in tissue engineering and regenerative medicine. MDPI 2023-10-24 /pmc/articles/PMC10669793/ /pubmed/38001872 http://dx.doi.org/10.3390/biomedicines11112871 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nakamura, Masayuki
Masuki, Hideo
Kawabata, Hideo
Watanabe, Taisuke
Watanabe, Takao
Tsujino, Tetsuhiro
Isobe, Kazushige
Kitamura, Yutaka
Mourão, Carlos Fernando
Kawase, Tomoyuki
Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate
title Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate
title_full Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate
title_fullStr Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate
title_full_unstemmed Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate
title_short Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate
title_sort plasma gel made of platelet-poor plasma: in vitro verification as a carrier of polyphosphate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669793/
https://www.ncbi.nlm.nih.gov/pubmed/38001872
http://dx.doi.org/10.3390/biomedicines11112871
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