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Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate
Plasma gel (PG) is a blood-derived biomaterial that can be prepared by heating or chemical cross-linking without the aid of intrinsic coagulation activity and has gradually been applied in the field of esthetic surgery. To explore the applicability of PG in regenerative therapy or tissue engineering...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669793/ https://www.ncbi.nlm.nih.gov/pubmed/38001872 http://dx.doi.org/10.3390/biomedicines11112871 |
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author | Nakamura, Masayuki Masuki, Hideo Kawabata, Hideo Watanabe, Taisuke Watanabe, Takao Tsujino, Tetsuhiro Isobe, Kazushige Kitamura, Yutaka Mourão, Carlos Fernando Kawase, Tomoyuki |
author_facet | Nakamura, Masayuki Masuki, Hideo Kawabata, Hideo Watanabe, Taisuke Watanabe, Takao Tsujino, Tetsuhiro Isobe, Kazushige Kitamura, Yutaka Mourão, Carlos Fernando Kawase, Tomoyuki |
author_sort | Nakamura, Masayuki |
collection | PubMed |
description | Plasma gel (PG) is a blood-derived biomaterial that can be prepared by heating or chemical cross-linking without the aid of intrinsic coagulation activity and has gradually been applied in the field of esthetic surgery. To explore the applicability of PG in regenerative therapy or tissue engineering, in this study, we focused on the advantages of the heating method and verified the retention capacity of the resulting PG for polyphosphate (polyP), a polyanion that contributes to hemostasis and bone regeneration. Pooled platelet-poor plasma (PPP) was prepared from four healthy male adult donors, mixed with synthetic polyP, and heated at 75 °C for 10 or 30 min to prepare PG in microtubes. The PG was incubated in PBS at 37 °C, and polyP levels in the extra-matrix PBS were determined by the fluorometric method every 24 h. The microstructure of PG was examined using scanning electron microscopy. In the small PG matrices, almost all of the added polyP (~100%) was released within the initial 24 h. In contrast, in the large PG matrices, approximately 50% of the polyP was released within the initial 24 h and thereafter gradually released over time. Owing to its simple chemical structure, linear polyP cannot be theoretically retained in the gel matrices used in this study. However, these findings suggest that thermally prepared PG matrices can be applied as carriers of polyP in tissue engineering and regenerative medicine. |
format | Online Article Text |
id | pubmed-10669793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106697932023-10-24 Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate Nakamura, Masayuki Masuki, Hideo Kawabata, Hideo Watanabe, Taisuke Watanabe, Takao Tsujino, Tetsuhiro Isobe, Kazushige Kitamura, Yutaka Mourão, Carlos Fernando Kawase, Tomoyuki Biomedicines Article Plasma gel (PG) is a blood-derived biomaterial that can be prepared by heating or chemical cross-linking without the aid of intrinsic coagulation activity and has gradually been applied in the field of esthetic surgery. To explore the applicability of PG in regenerative therapy or tissue engineering, in this study, we focused on the advantages of the heating method and verified the retention capacity of the resulting PG for polyphosphate (polyP), a polyanion that contributes to hemostasis and bone regeneration. Pooled platelet-poor plasma (PPP) was prepared from four healthy male adult donors, mixed with synthetic polyP, and heated at 75 °C for 10 or 30 min to prepare PG in microtubes. The PG was incubated in PBS at 37 °C, and polyP levels in the extra-matrix PBS were determined by the fluorometric method every 24 h. The microstructure of PG was examined using scanning electron microscopy. In the small PG matrices, almost all of the added polyP (~100%) was released within the initial 24 h. In contrast, in the large PG matrices, approximately 50% of the polyP was released within the initial 24 h and thereafter gradually released over time. Owing to its simple chemical structure, linear polyP cannot be theoretically retained in the gel matrices used in this study. However, these findings suggest that thermally prepared PG matrices can be applied as carriers of polyP in tissue engineering and regenerative medicine. MDPI 2023-10-24 /pmc/articles/PMC10669793/ /pubmed/38001872 http://dx.doi.org/10.3390/biomedicines11112871 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nakamura, Masayuki Masuki, Hideo Kawabata, Hideo Watanabe, Taisuke Watanabe, Takao Tsujino, Tetsuhiro Isobe, Kazushige Kitamura, Yutaka Mourão, Carlos Fernando Kawase, Tomoyuki Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate |
title | Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate |
title_full | Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate |
title_fullStr | Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate |
title_full_unstemmed | Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate |
title_short | Plasma Gel Made of Platelet-Poor Plasma: In Vitro Verification as a Carrier of Polyphosphate |
title_sort | plasma gel made of platelet-poor plasma: in vitro verification as a carrier of polyphosphate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10669793/ https://www.ncbi.nlm.nih.gov/pubmed/38001872 http://dx.doi.org/10.3390/biomedicines11112871 |
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