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Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol

Sperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced...

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Autores principales: Tachibana, Ryota, Takeuchi, Hiroki, Yoshikawa-Terada, Kento, Maezawa, Tadashi, Nishioka, Mikiko, Takayama, Erina, Tanaka, Hiroaki, Tanaka, Kayo, Hyon, Suong-hyu, Gen, Yuki, Kondo, Eiji, Ikeda, Tomoaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670029/
https://www.ncbi.nlm.nih.gov/pubmed/37998320
http://dx.doi.org/10.3390/cells12222585
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author Tachibana, Ryota
Takeuchi, Hiroki
Yoshikawa-Terada, Kento
Maezawa, Tadashi
Nishioka, Mikiko
Takayama, Erina
Tanaka, Hiroaki
Tanaka, Kayo
Hyon, Suong-hyu
Gen, Yuki
Kondo, Eiji
Ikeda, Tomoaki
author_facet Tachibana, Ryota
Takeuchi, Hiroki
Yoshikawa-Terada, Kento
Maezawa, Tadashi
Nishioka, Mikiko
Takayama, Erina
Tanaka, Hiroaki
Tanaka, Kayo
Hyon, Suong-hyu
Gen, Yuki
Kondo, Eiji
Ikeda, Tomoaki
author_sort Tachibana, Ryota
collection PubMed
description Sperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced SDF after freeze-thawing compared with clinically popular cryopreservation reagents containing human serum albumin. However, it is unclear whether CPLL reduces SDF, as it differed from the constituents of the commercial cryopreservation reagents used for comparison. Therefore, here, we examined whether CPLL reduces the SDF of human sperm and evaluated reactive oxygen species (ROS) levels and lipid peroxidation (LPO), which are the causes of SDF; mitochondrial injury, ROS production; and impaired sperm motility. Furthermore, optimal antioxidants and their concentrations that could further enhance the reduction in SDF were determined for future clinical application in ART and underwent the same functional evaluations. CPLL can reduce SDF via inhibition of intracytoplasmic ROS and LPO. Furthermore, the addition of 0.1 mM resveratrol avoided the enhancement of SDF, which potentially affects mitochondrial and cytoplasmic ROS and LPO. This novel human sperm cryopreservation reagent containing CPLL and resveratrol has the potential to improve treatment outcomes in ART using frozen sperm.
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spelling pubmed-106700292023-11-07 Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol Tachibana, Ryota Takeuchi, Hiroki Yoshikawa-Terada, Kento Maezawa, Tadashi Nishioka, Mikiko Takayama, Erina Tanaka, Hiroaki Tanaka, Kayo Hyon, Suong-hyu Gen, Yuki Kondo, Eiji Ikeda, Tomoaki Cells Article Sperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced SDF after freeze-thawing compared with clinically popular cryopreservation reagents containing human serum albumin. However, it is unclear whether CPLL reduces SDF, as it differed from the constituents of the commercial cryopreservation reagents used for comparison. Therefore, here, we examined whether CPLL reduces the SDF of human sperm and evaluated reactive oxygen species (ROS) levels and lipid peroxidation (LPO), which are the causes of SDF; mitochondrial injury, ROS production; and impaired sperm motility. Furthermore, optimal antioxidants and their concentrations that could further enhance the reduction in SDF were determined for future clinical application in ART and underwent the same functional evaluations. CPLL can reduce SDF via inhibition of intracytoplasmic ROS and LPO. Furthermore, the addition of 0.1 mM resveratrol avoided the enhancement of SDF, which potentially affects mitochondrial and cytoplasmic ROS and LPO. This novel human sperm cryopreservation reagent containing CPLL and resveratrol has the potential to improve treatment outcomes in ART using frozen sperm. MDPI 2023-11-07 /pmc/articles/PMC10670029/ /pubmed/37998320 http://dx.doi.org/10.3390/cells12222585 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tachibana, Ryota
Takeuchi, Hiroki
Yoshikawa-Terada, Kento
Maezawa, Tadashi
Nishioka, Mikiko
Takayama, Erina
Tanaka, Hiroaki
Tanaka, Kayo
Hyon, Suong-hyu
Gen, Yuki
Kondo, Eiji
Ikeda, Tomoaki
Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
title Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
title_full Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
title_fullStr Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
title_full_unstemmed Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
title_short Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
title_sort carboxylated poly-l-lysine potentially reduces human sperm dna fragmentation after freeze-thawing, and its function is enhanced by low-dose resveratrol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670029/
https://www.ncbi.nlm.nih.gov/pubmed/37998320
http://dx.doi.org/10.3390/cells12222585
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