Cargando…

Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy

Many children and adults who have suffered prenatal hypoxia at an early age develop many serious diseases. This disease is an actual problem of pediatric cardiology and little studied. The aim was to analyze the cardioprotective effect of L-arginine, Thiotriazoline, Angioline, and Mildronate on the...

Descripción completa

Detalles Bibliográficos
Autores principales: Popazova, Olena, Belenichev, Igor, Yadlovskyi, Oleh, Oksenych, Valentyn, Kamyshnyi, Aleksandr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670299/
https://www.ncbi.nlm.nih.gov/pubmed/37998724
http://dx.doi.org/10.3390/cimb45110547
_version_ 1785139891233882112
author Popazova, Olena
Belenichev, Igor
Yadlovskyi, Oleh
Oksenych, Valentyn
Kamyshnyi, Aleksandr
author_facet Popazova, Olena
Belenichev, Igor
Yadlovskyi, Oleh
Oksenych, Valentyn
Kamyshnyi, Aleksandr
author_sort Popazova, Olena
collection PubMed
description Many children and adults who have suffered prenatal hypoxia at an early age develop many serious diseases. This disease is an actual problem of pediatric cardiology and little studied. The aim was to analyze the cardioprotective effect of L-arginine, Thiotriazoline, Angioline, and Mildronate on the cardiovascular system of rats after prenatal hypoxia. Methods: The experiments were carried out on 50 female white rats; intraperitoneal sodium nitrite solution was administered daily to pregnant female rats after 16 days at a dose of 50 mg/kg. Control pregnant rats received saline. The offspring were divided into groups: 1—intact; 2—the control group of rat pups after PH, treated daily with physiological saline; 3—six groups of rat pups after PH, treated daily from the 1st to the 30th day after birth. Heat shock protein HSP70 was determined by enzyme immunoassay, ST2 Nitrotyrosine, and eNOS was observed by ELISA. Results: Angiolin showed a high cardioprotective effect even a month after discontinuation of the drug, and after introduction, the highest decrease in ST2 nitrotyrosine was revealed. Thiotriazoline and L-arginine have an antioxidant effect and a positive effect on eNOS expression, increasing the concentration of HSP70. Mildronate increased the expression of eNOS and the concentration of HSP70 in the blood of experimental rats after a course of administration, but did not show an antioxidant effect and did not reduce the concentration of nitrotyrosine. The results obtained indicate the cardioprotective effect of modulators of the NO system with different mechanisms of action of drugs after prenatal hypoxia.
format Online
Article
Text
id pubmed-10670299
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106702992023-10-30 Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy Popazova, Olena Belenichev, Igor Yadlovskyi, Oleh Oksenych, Valentyn Kamyshnyi, Aleksandr Curr Issues Mol Biol Communication Many children and adults who have suffered prenatal hypoxia at an early age develop many serious diseases. This disease is an actual problem of pediatric cardiology and little studied. The aim was to analyze the cardioprotective effect of L-arginine, Thiotriazoline, Angioline, and Mildronate on the cardiovascular system of rats after prenatal hypoxia. Methods: The experiments were carried out on 50 female white rats; intraperitoneal sodium nitrite solution was administered daily to pregnant female rats after 16 days at a dose of 50 mg/kg. Control pregnant rats received saline. The offspring were divided into groups: 1—intact; 2—the control group of rat pups after PH, treated daily with physiological saline; 3—six groups of rat pups after PH, treated daily from the 1st to the 30th day after birth. Heat shock protein HSP70 was determined by enzyme immunoassay, ST2 Nitrotyrosine, and eNOS was observed by ELISA. Results: Angiolin showed a high cardioprotective effect even a month after discontinuation of the drug, and after introduction, the highest decrease in ST2 nitrotyrosine was revealed. Thiotriazoline and L-arginine have an antioxidant effect and a positive effect on eNOS expression, increasing the concentration of HSP70. Mildronate increased the expression of eNOS and the concentration of HSP70 in the blood of experimental rats after a course of administration, but did not show an antioxidant effect and did not reduce the concentration of nitrotyrosine. The results obtained indicate the cardioprotective effect of modulators of the NO system with different mechanisms of action of drugs after prenatal hypoxia. MDPI 2023-10-30 /pmc/articles/PMC10670299/ /pubmed/37998724 http://dx.doi.org/10.3390/cimb45110547 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Popazova, Olena
Belenichev, Igor
Yadlovskyi, Oleh
Oksenych, Valentyn
Kamyshnyi, Aleksandr
Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy
title Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy
title_full Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy
title_fullStr Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy
title_full_unstemmed Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy
title_short Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy
title_sort altered blood molecular markers of cardiovascular function in rats after intrauterine hypoxia and drug therapy
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670299/
https://www.ncbi.nlm.nih.gov/pubmed/37998724
http://dx.doi.org/10.3390/cimb45110547
work_keys_str_mv AT popazovaolena alteredbloodmolecularmarkersofcardiovascularfunctioninratsafterintrauterinehypoxiaanddrugtherapy
AT belenichevigor alteredbloodmolecularmarkersofcardiovascularfunctioninratsafterintrauterinehypoxiaanddrugtherapy
AT yadlovskyioleh alteredbloodmolecularmarkersofcardiovascularfunctioninratsafterintrauterinehypoxiaanddrugtherapy
AT oksenychvalentyn alteredbloodmolecularmarkersofcardiovascularfunctioninratsafterintrauterinehypoxiaanddrugtherapy
AT kamyshnyialeksandr alteredbloodmolecularmarkersofcardiovascularfunctioninratsafterintrauterinehypoxiaanddrugtherapy