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Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts

Here, we, for the first time, compared the cardioprotective effects of third-generation vasodilating beta-blocker nebivolol (Neb) and conventional beta-blocker metoprolol (Met) on LPS-induced injury in H9c2 cardiomyoblasts. Our findings denoted that Neb and Met pretreatment diminish LPS-mediated cyt...

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Autores principales: Gul, Rukhsana, Okla, Meshail, Mahmood, Amer, Nawaz, Shahid, Fallata, Amina, Bazighifan, Arwa, Alfayez, Musaad, Alfadda, Assim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670410/
https://www.ncbi.nlm.nih.gov/pubmed/37998760
http://dx.doi.org/10.3390/cimb45110583
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author Gul, Rukhsana
Okla, Meshail
Mahmood, Amer
Nawaz, Shahid
Fallata, Amina
Bazighifan, Arwa
Alfayez, Musaad
Alfadda, Assim A.
author_facet Gul, Rukhsana
Okla, Meshail
Mahmood, Amer
Nawaz, Shahid
Fallata, Amina
Bazighifan, Arwa
Alfayez, Musaad
Alfadda, Assim A.
author_sort Gul, Rukhsana
collection PubMed
description Here, we, for the first time, compared the cardioprotective effects of third-generation vasodilating beta-blocker nebivolol (Neb) and conventional beta-blocker metoprolol (Met) on LPS-induced injury in H9c2 cardiomyoblasts. Our findings denoted that Neb and Met pretreatment diminish LPS-mediated cytotoxicity and oxidative stress. Concomitantly, LPS-triggered inflammatory cytokines activation was significantly suppressed by Neb but not by Met. Pretreatment with either Neb or Met alleviated LPS-mediated mitochondrial impairment by enhancing the expression of genes related to its biogenesis such as PGC-1α, NRF1, and TFAM. On the contrary, Neb but not Met-upregulated mitochondrial fusion-related genes such as OPA, and MFN2. In summary, our findings suggest that Neb and Met treatment significantly ameliorated the LPS-induced cytotoxicity and oxidative stress. Additionally, these findings suggest that Neb but not Met significantly down-regulates LPS-induced proinflammatory factors, probably by enhancing mitochondrial biogenesis and fusion.
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spelling pubmed-106704102023-11-20 Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts Gul, Rukhsana Okla, Meshail Mahmood, Amer Nawaz, Shahid Fallata, Amina Bazighifan, Arwa Alfayez, Musaad Alfadda, Assim A. Curr Issues Mol Biol Article Here, we, for the first time, compared the cardioprotective effects of third-generation vasodilating beta-blocker nebivolol (Neb) and conventional beta-blocker metoprolol (Met) on LPS-induced injury in H9c2 cardiomyoblasts. Our findings denoted that Neb and Met pretreatment diminish LPS-mediated cytotoxicity and oxidative stress. Concomitantly, LPS-triggered inflammatory cytokines activation was significantly suppressed by Neb but not by Met. Pretreatment with either Neb or Met alleviated LPS-mediated mitochondrial impairment by enhancing the expression of genes related to its biogenesis such as PGC-1α, NRF1, and TFAM. On the contrary, Neb but not Met-upregulated mitochondrial fusion-related genes such as OPA, and MFN2. In summary, our findings suggest that Neb and Met treatment significantly ameliorated the LPS-induced cytotoxicity and oxidative stress. Additionally, these findings suggest that Neb but not Met significantly down-regulates LPS-induced proinflammatory factors, probably by enhancing mitochondrial biogenesis and fusion. MDPI 2023-11-20 /pmc/articles/PMC10670410/ /pubmed/37998760 http://dx.doi.org/10.3390/cimb45110583 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gul, Rukhsana
Okla, Meshail
Mahmood, Amer
Nawaz, Shahid
Fallata, Amina
Bazighifan, Arwa
Alfayez, Musaad
Alfadda, Assim A.
Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts
title Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts
title_full Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts
title_fullStr Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts
title_full_unstemmed Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts
title_short Comparison of the Protective Effects of Nebivolol and Metoprolol against LPS-Induced Injury in H9c2 Cardiomyoblasts
title_sort comparison of the protective effects of nebivolol and metoprolol against lps-induced injury in h9c2 cardiomyoblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670410/
https://www.ncbi.nlm.nih.gov/pubmed/37998760
http://dx.doi.org/10.3390/cimb45110583
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