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Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study

Vaginal wall prolapse is the most common type of pelvic organ prolapse and is mainly associated with collagen bundle changes in the lamina propria. Neodymium (Nd:YAG) laser treatment was used as an innovative, minimally invasive and non-ablative procedure for the treatment of early-stage vaginal wal...

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Autores principales: Gubarkova, Ekaterina, Potapov, Arseniy, Moiseev, Alexander, Kiseleva, Elena, Krupinova, Darya, Shatilova, Ksenia, Karabut, Maria, Khlopkov, Andrey, Loginova, Maria, Radenska-Lopovok, Stefka, Gelikonov, Grigory, Grechkanev, Gennady, Gladkova, Natalia, Sirotkina, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670580/
https://www.ncbi.nlm.nih.gov/pubmed/37998623
http://dx.doi.org/10.3390/diagnostics13223487
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author Gubarkova, Ekaterina
Potapov, Arseniy
Moiseev, Alexander
Kiseleva, Elena
Krupinova, Darya
Shatilova, Ksenia
Karabut, Maria
Khlopkov, Andrey
Loginova, Maria
Radenska-Lopovok, Stefka
Gelikonov, Grigory
Grechkanev, Gennady
Gladkova, Natalia
Sirotkina, Marina
author_facet Gubarkova, Ekaterina
Potapov, Arseniy
Moiseev, Alexander
Kiseleva, Elena
Krupinova, Darya
Shatilova, Ksenia
Karabut, Maria
Khlopkov, Andrey
Loginova, Maria
Radenska-Lopovok, Stefka
Gelikonov, Grigory
Grechkanev, Gennady
Gladkova, Natalia
Sirotkina, Marina
author_sort Gubarkova, Ekaterina
collection PubMed
description Vaginal wall prolapse is the most common type of pelvic organ prolapse and is mainly associated with collagen bundle changes in the lamina propria. Neodymium (Nd:YAG) laser treatment was used as an innovative, minimally invasive and non-ablative procedure for the treatment of early-stage vaginal wall prolapse. The purpose of this pilot study was to assess connective tissue changes in the vaginal wall under prolapse without treatment and after Nd:YAG laser treatment using cross-polarization optical coherence tomography (CP OCT) with depth-resolved attenuation mapping. A total of 26 freshly excised samples of vaginal wall from 26 patients with age norm (n = 8), stage I–II prolapses without treatment (n = 8) and stage I–II prolapse 1–2 months after Nd:YAG laser treatment (n = 10) were assessed. As a result, for the first time, depth-resolved attenuation maps of the vaginal wall in the B-scan projection in the co- and cross-polarization channels were constructed. Two parameters within the lamina propria were target calculated: the median value and the percentages of high (≥4 mm(−1)) and low (<4 mm(−1)) attenuation coefficient values. A significant (p < 0.0001) decrease in the parameters in the case of vaginal wall prolapse compared to the age norm was identified. After laser treatment, a significant (p < 0.0001) increase in the parameters compared to the normal level was also observed. Notably, in the cross-channel, both parameters showed a greater difference between the groups than in the co-channel. Therefore, using the cross-channel achieved more reliable differentiation between the groups. To conclude, attenuation coefficient maps allow visualization and quantification of changes in the condition of the connective tissue of the vaginal wall. In the future, CP OCT could be used for in vivo detection of early-stage vaginal wall prolapse and for monitoring the effectiveness of treatment.
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spelling pubmed-106705802023-11-20 Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study Gubarkova, Ekaterina Potapov, Arseniy Moiseev, Alexander Kiseleva, Elena Krupinova, Darya Shatilova, Ksenia Karabut, Maria Khlopkov, Andrey Loginova, Maria Radenska-Lopovok, Stefka Gelikonov, Grigory Grechkanev, Gennady Gladkova, Natalia Sirotkina, Marina Diagnostics (Basel) Article Vaginal wall prolapse is the most common type of pelvic organ prolapse and is mainly associated with collagen bundle changes in the lamina propria. Neodymium (Nd:YAG) laser treatment was used as an innovative, minimally invasive and non-ablative procedure for the treatment of early-stage vaginal wall prolapse. The purpose of this pilot study was to assess connective tissue changes in the vaginal wall under prolapse without treatment and after Nd:YAG laser treatment using cross-polarization optical coherence tomography (CP OCT) with depth-resolved attenuation mapping. A total of 26 freshly excised samples of vaginal wall from 26 patients with age norm (n = 8), stage I–II prolapses without treatment (n = 8) and stage I–II prolapse 1–2 months after Nd:YAG laser treatment (n = 10) were assessed. As a result, for the first time, depth-resolved attenuation maps of the vaginal wall in the B-scan projection in the co- and cross-polarization channels were constructed. Two parameters within the lamina propria were target calculated: the median value and the percentages of high (≥4 mm(−1)) and low (<4 mm(−1)) attenuation coefficient values. A significant (p < 0.0001) decrease in the parameters in the case of vaginal wall prolapse compared to the age norm was identified. After laser treatment, a significant (p < 0.0001) increase in the parameters compared to the normal level was also observed. Notably, in the cross-channel, both parameters showed a greater difference between the groups than in the co-channel. Therefore, using the cross-channel achieved more reliable differentiation between the groups. To conclude, attenuation coefficient maps allow visualization and quantification of changes in the condition of the connective tissue of the vaginal wall. In the future, CP OCT could be used for in vivo detection of early-stage vaginal wall prolapse and for monitoring the effectiveness of treatment. MDPI 2023-11-20 /pmc/articles/PMC10670580/ /pubmed/37998623 http://dx.doi.org/10.3390/diagnostics13223487 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gubarkova, Ekaterina
Potapov, Arseniy
Moiseev, Alexander
Kiseleva, Elena
Krupinova, Darya
Shatilova, Ksenia
Karabut, Maria
Khlopkov, Andrey
Loginova, Maria
Radenska-Lopovok, Stefka
Gelikonov, Grigory
Grechkanev, Gennady
Gladkova, Natalia
Sirotkina, Marina
Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study
title Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study
title_full Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study
title_fullStr Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study
title_full_unstemmed Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study
title_short Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study
title_sort depth-resolved attenuation mapping of the vaginal wall under prolapse and after laser treatment using cross-polarization optical coherence tomography: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670580/
https://www.ncbi.nlm.nih.gov/pubmed/37998623
http://dx.doi.org/10.3390/diagnostics13223487
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