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Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials

Electroretinograms (ERGs) are often used to evaluate retinal function. However, assessing local retinal function can be challenging; therefore, photopic and scotopic ERGs are used to record whole-retinal function. This study evaluated focal retinal function in rats exposed to continuous light using...

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Autores principales: Takita, Yuya, Sugano, Eriko, Kitabayashi, Kazuya, Tabata, Kitako, Saito, Akito, Yokoyama, Takanori, Onoguchi, Reina, Fukuda, Tomokazu, Ozaki, Taku, Bai, Lanlan, Tomita, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670973/
https://www.ncbi.nlm.nih.gov/pubmed/38003623
http://dx.doi.org/10.3390/ijms242216433
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author Takita, Yuya
Sugano, Eriko
Kitabayashi, Kazuya
Tabata, Kitako
Saito, Akito
Yokoyama, Takanori
Onoguchi, Reina
Fukuda, Tomokazu
Ozaki, Taku
Bai, Lanlan
Tomita, Hiroshi
author_facet Takita, Yuya
Sugano, Eriko
Kitabayashi, Kazuya
Tabata, Kitako
Saito, Akito
Yokoyama, Takanori
Onoguchi, Reina
Fukuda, Tomokazu
Ozaki, Taku
Bai, Lanlan
Tomita, Hiroshi
author_sort Takita, Yuya
collection PubMed
description Electroretinograms (ERGs) are often used to evaluate retinal function. However, assessing local retinal function can be challenging; therefore, photopic and scotopic ERGs are used to record whole-retinal function. This study evaluated focal retinal function in rats exposed to continuous light using a multifocal ERG (mfERG) system. The rats were exposed to 1000 lux of fluorescent light for 24 h to induce photoreceptor degeneration. After light exposure, the rats were reared under cyclic light conditions (12 h: 5 lux, 12 h: dark). Photopic and multifocal ERGs and single-flash and multifocal visual evoked potentials (mfVEPs) were recorded 7 days after light exposure. Fourteen days following light exposure, paraffin-embedded sections were prepared from the eyes for histological evaluation. The ERG and VEP responses dramatically decreased after 24 h of light exposure, and retinal area-dependent decreases were observed in mfERGs and mfVEPs. Histological assessment revealed severe damage to the superior retina and less damage to the inferior retina. Considering the recorded visual angles of mfERGs and mfVEPs, the degenerated area shown on the histological examinations correlates well with the responses from multifocal recordings.
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spelling pubmed-106709732023-11-17 Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials Takita, Yuya Sugano, Eriko Kitabayashi, Kazuya Tabata, Kitako Saito, Akito Yokoyama, Takanori Onoguchi, Reina Fukuda, Tomokazu Ozaki, Taku Bai, Lanlan Tomita, Hiroshi Int J Mol Sci Article Electroretinograms (ERGs) are often used to evaluate retinal function. However, assessing local retinal function can be challenging; therefore, photopic and scotopic ERGs are used to record whole-retinal function. This study evaluated focal retinal function in rats exposed to continuous light using a multifocal ERG (mfERG) system. The rats were exposed to 1000 lux of fluorescent light for 24 h to induce photoreceptor degeneration. After light exposure, the rats were reared under cyclic light conditions (12 h: 5 lux, 12 h: dark). Photopic and multifocal ERGs and single-flash and multifocal visual evoked potentials (mfVEPs) were recorded 7 days after light exposure. Fourteen days following light exposure, paraffin-embedded sections were prepared from the eyes for histological evaluation. The ERG and VEP responses dramatically decreased after 24 h of light exposure, and retinal area-dependent decreases were observed in mfERGs and mfVEPs. Histological assessment revealed severe damage to the superior retina and less damage to the inferior retina. Considering the recorded visual angles of mfERGs and mfVEPs, the degenerated area shown on the histological examinations correlates well with the responses from multifocal recordings. MDPI 2023-11-17 /pmc/articles/PMC10670973/ /pubmed/38003623 http://dx.doi.org/10.3390/ijms242216433 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takita, Yuya
Sugano, Eriko
Kitabayashi, Kazuya
Tabata, Kitako
Saito, Akito
Yokoyama, Takanori
Onoguchi, Reina
Fukuda, Tomokazu
Ozaki, Taku
Bai, Lanlan
Tomita, Hiroshi
Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials
title Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials
title_full Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials
title_fullStr Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials
title_full_unstemmed Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials
title_short Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials
title_sort evaluation of local retinal function in light-damaged rats using multifocal electroretinograms and multifocal visual evoked potentials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670973/
https://www.ncbi.nlm.nih.gov/pubmed/38003623
http://dx.doi.org/10.3390/ijms242216433
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