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Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials
Electroretinograms (ERGs) are often used to evaluate retinal function. However, assessing local retinal function can be challenging; therefore, photopic and scotopic ERGs are used to record whole-retinal function. This study evaluated focal retinal function in rats exposed to continuous light using...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670973/ https://www.ncbi.nlm.nih.gov/pubmed/38003623 http://dx.doi.org/10.3390/ijms242216433 |
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author | Takita, Yuya Sugano, Eriko Kitabayashi, Kazuya Tabata, Kitako Saito, Akito Yokoyama, Takanori Onoguchi, Reina Fukuda, Tomokazu Ozaki, Taku Bai, Lanlan Tomita, Hiroshi |
author_facet | Takita, Yuya Sugano, Eriko Kitabayashi, Kazuya Tabata, Kitako Saito, Akito Yokoyama, Takanori Onoguchi, Reina Fukuda, Tomokazu Ozaki, Taku Bai, Lanlan Tomita, Hiroshi |
author_sort | Takita, Yuya |
collection | PubMed |
description | Electroretinograms (ERGs) are often used to evaluate retinal function. However, assessing local retinal function can be challenging; therefore, photopic and scotopic ERGs are used to record whole-retinal function. This study evaluated focal retinal function in rats exposed to continuous light using a multifocal ERG (mfERG) system. The rats were exposed to 1000 lux of fluorescent light for 24 h to induce photoreceptor degeneration. After light exposure, the rats were reared under cyclic light conditions (12 h: 5 lux, 12 h: dark). Photopic and multifocal ERGs and single-flash and multifocal visual evoked potentials (mfVEPs) were recorded 7 days after light exposure. Fourteen days following light exposure, paraffin-embedded sections were prepared from the eyes for histological evaluation. The ERG and VEP responses dramatically decreased after 24 h of light exposure, and retinal area-dependent decreases were observed in mfERGs and mfVEPs. Histological assessment revealed severe damage to the superior retina and less damage to the inferior retina. Considering the recorded visual angles of mfERGs and mfVEPs, the degenerated area shown on the histological examinations correlates well with the responses from multifocal recordings. |
format | Online Article Text |
id | pubmed-10670973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106709732023-11-17 Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials Takita, Yuya Sugano, Eriko Kitabayashi, Kazuya Tabata, Kitako Saito, Akito Yokoyama, Takanori Onoguchi, Reina Fukuda, Tomokazu Ozaki, Taku Bai, Lanlan Tomita, Hiroshi Int J Mol Sci Article Electroretinograms (ERGs) are often used to evaluate retinal function. However, assessing local retinal function can be challenging; therefore, photopic and scotopic ERGs are used to record whole-retinal function. This study evaluated focal retinal function in rats exposed to continuous light using a multifocal ERG (mfERG) system. The rats were exposed to 1000 lux of fluorescent light for 24 h to induce photoreceptor degeneration. After light exposure, the rats were reared under cyclic light conditions (12 h: 5 lux, 12 h: dark). Photopic and multifocal ERGs and single-flash and multifocal visual evoked potentials (mfVEPs) were recorded 7 days after light exposure. Fourteen days following light exposure, paraffin-embedded sections were prepared from the eyes for histological evaluation. The ERG and VEP responses dramatically decreased after 24 h of light exposure, and retinal area-dependent decreases were observed in mfERGs and mfVEPs. Histological assessment revealed severe damage to the superior retina and less damage to the inferior retina. Considering the recorded visual angles of mfERGs and mfVEPs, the degenerated area shown on the histological examinations correlates well with the responses from multifocal recordings. MDPI 2023-11-17 /pmc/articles/PMC10670973/ /pubmed/38003623 http://dx.doi.org/10.3390/ijms242216433 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Takita, Yuya Sugano, Eriko Kitabayashi, Kazuya Tabata, Kitako Saito, Akito Yokoyama, Takanori Onoguchi, Reina Fukuda, Tomokazu Ozaki, Taku Bai, Lanlan Tomita, Hiroshi Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials |
title | Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials |
title_full | Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials |
title_fullStr | Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials |
title_full_unstemmed | Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials |
title_short | Evaluation of Local Retinal Function in Light-Damaged Rats Using Multifocal Electroretinograms and Multifocal Visual Evoked Potentials |
title_sort | evaluation of local retinal function in light-damaged rats using multifocal electroretinograms and multifocal visual evoked potentials |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670973/ https://www.ncbi.nlm.nih.gov/pubmed/38003623 http://dx.doi.org/10.3390/ijms242216433 |
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