Cargando…
Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches
The era of increasing bacterial antibiotic resistance requires new approaches to fight infections. With this purpose, silver-based nanomaterials are a reality in some fields and promise new developments. We report the green synthesis of silver nanoparticles (AgNPs) using culture broths from a microa...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670984/ https://www.ncbi.nlm.nih.gov/pubmed/38003373 http://dx.doi.org/10.3390/ijms242216183 |
_version_ | 1785140048614653952 |
---|---|
author | Pernas-Pleite, Carlos Conejo-Martínez, Amparo M. Fernández Freire, Paloma Hazen, María José Marín, Irma Abad, José P. |
author_facet | Pernas-Pleite, Carlos Conejo-Martínez, Amparo M. Fernández Freire, Paloma Hazen, María José Marín, Irma Abad, José P. |
author_sort | Pernas-Pleite, Carlos |
collection | PubMed |
description | The era of increasing bacterial antibiotic resistance requires new approaches to fight infections. With this purpose, silver-based nanomaterials are a reality in some fields and promise new developments. We report the green synthesis of silver nanoparticles (AgNPs) using culture broths from a microalga. Broths from two media, with different compositions and pHs and sampled at two growth phases, produced eight AgNP types. Nanoparticles harvested after several synthesis periods showed differences in antibacterial activity and stability. Moreover, an evaluation of the broths for several consecutive syntheses did not find relevant kinetics or activity differences until the third round. Physicochemical characteristics of the AgNPs (core and hydrodynamic sizes, Z-potential, crystallinity, and corona composition) were determined, observing differences depending on the broths used. AgNPs showed good antibacterial activity at concentrations producing no or low cytotoxicity on cultured eukaryotic cells. All the AgNPs had high levels of synergy against Escherichia coli and Staphylococcus aureus with the classic antibiotics streptomycin and kanamycin, but with ampicillin only against S. aureus and tetracycline against E. coli. Differences in the synergy levels were also dependent on the types of AgNPs. We also found that, for some AgNPs, the killing of bacteria started before the massive accumulation of ROS. |
format | Online Article Text |
id | pubmed-10670984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106709842023-11-10 Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches Pernas-Pleite, Carlos Conejo-Martínez, Amparo M. Fernández Freire, Paloma Hazen, María José Marín, Irma Abad, José P. Int J Mol Sci Article The era of increasing bacterial antibiotic resistance requires new approaches to fight infections. With this purpose, silver-based nanomaterials are a reality in some fields and promise new developments. We report the green synthesis of silver nanoparticles (AgNPs) using culture broths from a microalga. Broths from two media, with different compositions and pHs and sampled at two growth phases, produced eight AgNP types. Nanoparticles harvested after several synthesis periods showed differences in antibacterial activity and stability. Moreover, an evaluation of the broths for several consecutive syntheses did not find relevant kinetics or activity differences until the third round. Physicochemical characteristics of the AgNPs (core and hydrodynamic sizes, Z-potential, crystallinity, and corona composition) were determined, observing differences depending on the broths used. AgNPs showed good antibacterial activity at concentrations producing no or low cytotoxicity on cultured eukaryotic cells. All the AgNPs had high levels of synergy against Escherichia coli and Staphylococcus aureus with the classic antibiotics streptomycin and kanamycin, but with ampicillin only against S. aureus and tetracycline against E. coli. Differences in the synergy levels were also dependent on the types of AgNPs. We also found that, for some AgNPs, the killing of bacteria started before the massive accumulation of ROS. MDPI 2023-11-10 /pmc/articles/PMC10670984/ /pubmed/38003373 http://dx.doi.org/10.3390/ijms242216183 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pernas-Pleite, Carlos Conejo-Martínez, Amparo M. Fernández Freire, Paloma Hazen, María José Marín, Irma Abad, José P. Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches |
title | Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches |
title_full | Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches |
title_fullStr | Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches |
title_full_unstemmed | Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches |
title_short | Microalga Broths Synthesize Antibacterial and Non-Cytotoxic Silver Nanoparticles Showing Synergy with Antibiotics and Bacterial ROS Induction and Can Be Reused for Successive AgNP Batches |
title_sort | microalga broths synthesize antibacterial and non-cytotoxic silver nanoparticles showing synergy with antibiotics and bacterial ros induction and can be reused for successive agnp batches |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670984/ https://www.ncbi.nlm.nih.gov/pubmed/38003373 http://dx.doi.org/10.3390/ijms242216183 |
work_keys_str_mv | AT pernaspleitecarlos microalgabrothssynthesizeantibacterialandnoncytotoxicsilvernanoparticlesshowingsynergywithantibioticsandbacterialrosinductionandcanbereusedforsuccessiveagnpbatches AT conejomartinezamparom microalgabrothssynthesizeantibacterialandnoncytotoxicsilvernanoparticlesshowingsynergywithantibioticsandbacterialrosinductionandcanbereusedforsuccessiveagnpbatches AT fernandezfreirepaloma microalgabrothssynthesizeantibacterialandnoncytotoxicsilvernanoparticlesshowingsynergywithantibioticsandbacterialrosinductionandcanbereusedforsuccessiveagnpbatches AT hazenmariajose microalgabrothssynthesizeantibacterialandnoncytotoxicsilvernanoparticlesshowingsynergywithantibioticsandbacterialrosinductionandcanbereusedforsuccessiveagnpbatches AT marinirma microalgabrothssynthesizeantibacterialandnoncytotoxicsilvernanoparticlesshowingsynergywithantibioticsandbacterialrosinductionandcanbereusedforsuccessiveagnpbatches AT abadjosep microalgabrothssynthesizeantibacterialandnoncytotoxicsilvernanoparticlesshowingsynergywithantibioticsandbacterialrosinductionandcanbereusedforsuccessiveagnpbatches |