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Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells

Prostate cancer (PCa) is the second most common male cancer. Its incidence derives from the interaction between modifiable and non-modifiable factors. The progression of prostate cancer into a more aggressive phenotype is associated with chronic inflammation and increased ROS production. For their b...

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Autores principales: Giordano, Francesca, Comità, Stefano, Venneri, Giulia, Rago, Vittoria, Naimo, Giuseppina Daniela, De Amicis, Francesca, De Bartolo, Anna, Tundis, Rosa, Mauro, Loredana, Panno, Maria Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671002/
https://www.ncbi.nlm.nih.gov/pubmed/38003541
http://dx.doi.org/10.3390/ijms242216351
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author Giordano, Francesca
Comità, Stefano
Venneri, Giulia
Rago, Vittoria
Naimo, Giuseppina Daniela
De Amicis, Francesca
De Bartolo, Anna
Tundis, Rosa
Mauro, Loredana
Panno, Maria Luisa
author_facet Giordano, Francesca
Comità, Stefano
Venneri, Giulia
Rago, Vittoria
Naimo, Giuseppina Daniela
De Amicis, Francesca
De Bartolo, Anna
Tundis, Rosa
Mauro, Loredana
Panno, Maria Luisa
author_sort Giordano, Francesca
collection PubMed
description Prostate cancer (PCa) is the second most common male cancer. Its incidence derives from the interaction between modifiable and non-modifiable factors. The progression of prostate cancer into a more aggressive phenotype is associated with chronic inflammation and increased ROS production. For their biological properties, some phytochemicals from fruits and vegetable emerge as a promise strategy for cancer progression delay. These bioactive compounds are found in the highest amounts in peels and seeds. Poncirus trifoliata (L.) Raf. (PT) has been widely used in traditional medicine and retains anti-inflammatory, anti-bacterial, and anticancer effects. The seeds of P. trifoliata were exhaustively extracted by maceration with methanol as the solvent. The cell proliferation rate was performed by MTT and flow cytometry, while the apoptosis signals were analyzed by Western blotting and TUNEL assay. P. trifoliata seed extract reduced LNCaP and PC3 cell viability and induced cell cycle arrest at the G0/G1phase and apoptosis. In addition, a reduction in the AKT/mTOR pathway has been observed together with the up-regulation of stress-activated MAPK (p38 and c-Jun N-terminal kinase). Based on the study, the anti-growth effects of PT seed extract on prostate tumor cells give indications on the potential of the phytochemical drug for the treatment of this type of cancer. However, future in-depth studies are necessary to identify which components are mainly responsible for the anti-neoplastic response.
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spelling pubmed-106710022023-11-15 Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells Giordano, Francesca Comità, Stefano Venneri, Giulia Rago, Vittoria Naimo, Giuseppina Daniela De Amicis, Francesca De Bartolo, Anna Tundis, Rosa Mauro, Loredana Panno, Maria Luisa Int J Mol Sci Article Prostate cancer (PCa) is the second most common male cancer. Its incidence derives from the interaction between modifiable and non-modifiable factors. The progression of prostate cancer into a more aggressive phenotype is associated with chronic inflammation and increased ROS production. For their biological properties, some phytochemicals from fruits and vegetable emerge as a promise strategy for cancer progression delay. These bioactive compounds are found in the highest amounts in peels and seeds. Poncirus trifoliata (L.) Raf. (PT) has been widely used in traditional medicine and retains anti-inflammatory, anti-bacterial, and anticancer effects. The seeds of P. trifoliata were exhaustively extracted by maceration with methanol as the solvent. The cell proliferation rate was performed by MTT and flow cytometry, while the apoptosis signals were analyzed by Western blotting and TUNEL assay. P. trifoliata seed extract reduced LNCaP and PC3 cell viability and induced cell cycle arrest at the G0/G1phase and apoptosis. In addition, a reduction in the AKT/mTOR pathway has been observed together with the up-regulation of stress-activated MAPK (p38 and c-Jun N-terminal kinase). Based on the study, the anti-growth effects of PT seed extract on prostate tumor cells give indications on the potential of the phytochemical drug for the treatment of this type of cancer. However, future in-depth studies are necessary to identify which components are mainly responsible for the anti-neoplastic response. MDPI 2023-11-15 /pmc/articles/PMC10671002/ /pubmed/38003541 http://dx.doi.org/10.3390/ijms242216351 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giordano, Francesca
Comità, Stefano
Venneri, Giulia
Rago, Vittoria
Naimo, Giuseppina Daniela
De Amicis, Francesca
De Bartolo, Anna
Tundis, Rosa
Mauro, Loredana
Panno, Maria Luisa
Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells
title Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells
title_full Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells
title_fullStr Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells
title_full_unstemmed Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells
title_short Poncirus trifoliata (L.) Raf. Seed Extract Induces Cell Cycle Arrest and Apoptosis in the Androgen Receptor Positive LNCaP Prostate Cancer Cells
title_sort poncirus trifoliata (l.) raf. seed extract induces cell cycle arrest and apoptosis in the androgen receptor positive lncap prostate cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671002/
https://www.ncbi.nlm.nih.gov/pubmed/38003541
http://dx.doi.org/10.3390/ijms242216351
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