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Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review

Intravital microscopy (IVM) is a powerful imaging tool that captures biological processes in real-time. IVM facilitates the observation of complex cellular interactions in vivo, where ex vivo and in vitro experiments lack the physiological environment. IVM has been used in a multitude of studies und...

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Autores principales: Scott, Cassidy, Neira Agonh, Daniel, White, Hannah, Sultana, Saki, Lehmann, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671110/
https://www.ncbi.nlm.nih.gov/pubmed/38003533
http://dx.doi.org/10.3390/ijms242216345
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author Scott, Cassidy
Neira Agonh, Daniel
White, Hannah
Sultana, Saki
Lehmann, Christian
author_facet Scott, Cassidy
Neira Agonh, Daniel
White, Hannah
Sultana, Saki
Lehmann, Christian
author_sort Scott, Cassidy
collection PubMed
description Intravital microscopy (IVM) is a powerful imaging tool that captures biological processes in real-time. IVM facilitates the observation of complex cellular interactions in vivo, where ex vivo and in vitro experiments lack the physiological environment. IVM has been used in a multitude of studies under healthy and pathological conditions in different organ systems. IVM has become essential in the characterization of the immune response through visualization of leukocyte–endothelial interactions and subsequent changes within the microcirculation. Lipopolysaccharide (LPS), a common inflammatory trigger, has been used to induce inflammatory changes in various studies utilizing IVM. In this review, we provide an overview of IVM imaging of LPS-induced inflammation in different models, such as the brain, intestines, bladder, and lungs.
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spelling pubmed-106711102023-11-15 Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review Scott, Cassidy Neira Agonh, Daniel White, Hannah Sultana, Saki Lehmann, Christian Int J Mol Sci Review Intravital microscopy (IVM) is a powerful imaging tool that captures biological processes in real-time. IVM facilitates the observation of complex cellular interactions in vivo, where ex vivo and in vitro experiments lack the physiological environment. IVM has been used in a multitude of studies under healthy and pathological conditions in different organ systems. IVM has become essential in the characterization of the immune response through visualization of leukocyte–endothelial interactions and subsequent changes within the microcirculation. Lipopolysaccharide (LPS), a common inflammatory trigger, has been used to induce inflammatory changes in various studies utilizing IVM. In this review, we provide an overview of IVM imaging of LPS-induced inflammation in different models, such as the brain, intestines, bladder, and lungs. MDPI 2023-11-15 /pmc/articles/PMC10671110/ /pubmed/38003533 http://dx.doi.org/10.3390/ijms242216345 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Scott, Cassidy
Neira Agonh, Daniel
White, Hannah
Sultana, Saki
Lehmann, Christian
Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review
title Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review
title_full Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review
title_fullStr Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review
title_full_unstemmed Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review
title_short Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review
title_sort intravital microscopy of lipopolysaccharide-induced inflammatory changes in different organ systems—a scoping review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671110/
https://www.ncbi.nlm.nih.gov/pubmed/38003533
http://dx.doi.org/10.3390/ijms242216345
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