Cargando…

Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns of WRKY1 Transcription Factor and Rhynchophylline Biosynthesis-Related Genes in Uncaria rhynchophylla

Uncaria rhynchophylla (Miq.) Miq. ex Havil, a traditional medicinal herb, is enriched with several pharmacologically active terpenoid indole alkaloids (TIAs). At present, no method has been reported that can comprehensively select and evaluate the appropriate reference genes for gene expression anal...

Descripción completa

Detalles Bibliográficos
Autores principales:	Mu, Detian, Shao, Yingying, He, Jialong, Zhu, Lina, Qiu, Deyou, Wilson, Iain W., Zhang, Yao, Pan, Limei, Zhou, Yu, Lu, Ying, Tang, Qi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671239/ https://www.ncbi.nlm.nih.gov/pubmed/38003520 http://dx.doi.org/10.3390/ijms242216330

Ejemplares similares

Profiles of Metabolic Genes in Uncaria rhynchophylla and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline
por: Yang, Mengquan, et al.
Publicado: (2022)

Optimization of Isolation and Transformation of Protoplasts from Uncaria rhynchophylla and Its Application to Transient Gene Expression Analysis
por: Shao, Yingying, et al.
Publicado: (2023)

De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla, a non-model plant with potent anti-alzheimer’s properties
por: Guo, Qianqian, et al.
Publicado: (2014)

Comparison of the chemical constituents and anti-Alzheimer’s disease effects of Uncaria rhynchophylla and Uncaria tomentosa
por: Xu, Qing-Qing, et al.
Publicado: (2021)

Antiepileptic Effect of Uncaria rhynchophylla and Rhynchophylline Involved in the Initiation of c-Jun N-Terminal Kinase Phosphorylation of MAPK Signal Pathways in Acute Seizures of Kainic Acid-Treated Rats
por: Hsu, Hsin-Cheng, et al.
Publicado: (2013)

Indole Alkaloids Inhibiting Neural Stem Cell from Uncaria rhynchophylla
por: Wei, Xin, et al.
Publicado: (2017)

The complete chloroplast genome of the traditional Chinese herb, Uncaria rhynchophylla (Rubiaceae)
por: Ling, Li-Zhen, et al.
Publicado: (2020)

Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
por: Shin, Mi-Rae, et al.
Publicado: (2021)

Uncarialines A-E, new alkaloids from Uncaria rhynchophylla and their anticoagulant activity
por: Huang, Ke-Pu, et al.
Publicado: (2023)

Identification of Uncaria rhynchophylla in the Potential Treatment of Alzheimer’s Disease by Integrating Virtual Screening and In Vitro Validation
por: Jiang, Shuang, et al.
Publicado: (2023)

Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Siraitia siamensis
por: Mu, Detian, et al.
Publicado: (2023)

Hirsutine, an Indole Alkaloid of Uncaria rhynchophylla, Inhibits Late Step in Dengue Virus Lifecycle
por: Hishiki, Takayuki, et al.
Publicado: (2017)

Correction: Uncarialines A-E, new alkaloids from Uncaria rhynchophylla and their anticoagulant activity
por: Huang, Ke‑Pu, et al.
Publicado: (2023)

Validated Quantitative (1)H NMR Method for Simultaneous Quantification of Indole Alkaloids in Uncaria rhynchophylla
por: Yin, Tianpeng, et al.
Publicado: (2021)

Therapeutic Mechanism and Key Alkaloids of Uncaria rhynchophylla in Alzheimer’s Disease From the Perspective of Pathophysiological Processes
por: Zeng, Peng, et al.
Publicado: (2021)

Neuroprotection Against MPP(+)-Induced Cytotoxicity Through the Activation of PI3-K/Akt/GSK3β/MEF2D Signaling Pathway by Rhynchophylline, the Major Tetracyclic Oxindole Alkaloid Isolated From Uncaria rhynchophylla
por: Hu, Shengquan, et al.
Publicado: (2018)

Bioassay-Guided Isolation of Neuroprotective Compounds from Uncaria rhynchophylla against Beta-Amyloid-Induced Neurotoxicity
por: Xian, Yan-Fang, et al.
Publicado: (2012)

The Main Alkaloids in Uncaria rhynchophylla and Their Anti-Alzheimer’s Disease Mechanism Determined by a Network Pharmacology Approach
por: Zeng, Peng, et al.
Publicado: (2021)

The effects of genetic variation and environmental factors on rhynchophylline and isorhynchophylline in Uncaria macrophylla Wall. from different populations in China
por: Song, Mei-Fang, et al.
Publicado: (2018)

Evaluation of the effects of Uncaria rhynchophylla alkaloid extract on LPS-induced preeclampsia symptoms and inflammation in a pregnant rat model
por: Wu, Liang-Zhi, et al.
Publicado: (2019)

Soil Heavy Metal Content and Enzyme Activity in Uncaria rhynchophylla-Producing Areas under Different Land Use Patterns
por: Yang, Xiuyuan, et al.
Publicado: (2022)

Preparative Separation of Six Rhynchophylla Alkaloids from Uncaria macrophylla Wall by pH-Zone Refining Counter-Current Chromatography
por: Zhang, Qinghai, et al.
Publicado: (2013)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

RT-qPCR Normalization Genes in the Red Alga Chondrus crispus
por: Kowalczyk, Nathalie, et al.
Publicado: (2014)

Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
por: Köhsler, Martina, et al.
Publicado: (2020)

Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi
por: Martín-Pérez, Tania, et al.
Publicado: (2023)

Long-Term Intake of Uncaria rhynchophylla Reduces S100B and RAGE Protein Levels in Kainic Acid-Induced Epileptic Seizures Rats
por: Tang, Nou-Ying, et al.
Publicado: (2017)

Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments in Gelsemium elegans
por: Zhang, Yao, et al.
Publicado: (2023)

Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)
por: Jeong, Jae-Kyo, et al.
Publicado: (2014)

Accurate RT-qPCR gene expression analysis on cell culture lysates
por: Van Peer, Gert, et al.
Publicado: (2012)

Assessment of brain reference genes for RT-qPCR studies in neurodegenerative diseases
por: Rydbirk, Rasmus, et al.
Publicado: (2016)

Assessment of suitable reference genes for RT–qPCR studies in chronic rhinosinusitis
por: Nakayama, Tsuguhisa, et al.
Publicado: (2018)

Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes
por: Tsuchiya, Koji, et al.
Publicado: (2018)

Identification of reference genes for RT-qPCR data normalisation in aging studies
por: González-Bermúdez, Lourdes, et al.
Publicado: (2019)

Identification and validation of reference genes for RT-qPCR normalization in wheat meiosis
por: Garrido, José, et al.
Publicado: (2020)

Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes
por: Na, Wei, et al.
Publicado: (2021)

Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions
por: Svingen, Terje, et al.
Publicado: (2015)

Uncarinic Acid C Isolated from Uncaria rhynchophylla Induces Differentiation of Th1-Promoting Dendritic Cells Through TLR4 Signaling
por: Kim, Kyu Sik, et al.
Publicado: (2011)

Screening Reliable Reference Genes for RT-qPCR Analysis of Gene Expression in Moringa oleifera
por: Deng, Li-Ting, et al.
Publicado: (2016)

Selection and Validation of Candidate Reference Genes for Gene Expression Analysis by RT-qPCR in Rubus
por: Wu, Yaqiong, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_fr90agkvn549oasihses8i59os